4.2.2.21: chondroitin-sulfate-ABC exolyase

This is an abbreviated version!
For detailed information about chondroitin-sulfate-ABC exolyase, go to the full flat file.

Reaction

Synonyms

BactnABC, cABCII, chABC, Chondroitin Sulfate Lyase ABC, chondroitinase ABC, chondroitinase ABC II, chondroitinase AC, chondroitinase ACII, chondroitinase-ABC, ChS ABC lyase II, EC 4.2.2.4, EC 4.2.99.6, HCLase

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.2 Acting on polysaccharides

                4.2.2.21 chondroitin-sulfate-ABC exolyase

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Results	in table
3	Activating Compound
20	AA Sequence
9	Application
4	Cloned(Commentary)
1	Crystallization (Commentary)
2	General Stability
16	Inhibitors
7	kcat/KM [mM/s]
56	KM Value [mM]
1	Localization
8	Metals/Ions
11	Molecular Weight [Da]
31	Organism
4	Pathway
2	PDB ID
17	pH Optimum
2	pH Range
5	pI Value
45	Protein Variants
8	Purification (Commentary)
3	Reaction
28	Reference
1	Source Tissue
21	Specific Activity [micromol/min/mg]
2	Storage Stability
88	Substrates and Products (Substrate)
7	Subunits
22	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
2	Temperature Range [°C]
4	Temperature Stability [°C]
55	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 4.2.2.21 - chondroitin-sulfate-ABC exolyase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

2-aminobenzamide-labeled chondroitin sulfate-C hexasaccharide

unsaturated 2-aminobenzamide-labeled chondroitin sulfate-C disaccharide + ?

Vibrio sp. FC509

A0A077ERI8

-

747033

-

-

?

2-aminobenzamide-labeled chondroitin sulfate-C tetrasaccharide

unsaturated 2-aminobenzamide-labeled chondroitin sulfate-C disaccharide + disaccharide

Vibrio sp. FC509

A0A077ERI8

-

747033

-

-

?

bovine nasal cartilage proteoglycan

2-acetamido-2-deoxy-3-O-(3-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose + bovine nasal cartilage proteoglycan core

2 entries

chondroitin

?

4 entries

chondroitin 4-sulfate

?

3 entries

chondroitin 4-sulfate

unsaturated chondroitin 4-sulfate disaccharide

2 entries

chondroitin 6-sulfate

?

Proteus vulgaris

-

-

704643

-

-

?

chondroitin 6-sulfate

unsaturated chondroitin 6-sulfate disaccharide

2 entries

chondroitin sulfate

unsaturated sulfated chondroitin disaccharide

Pedobacter heparinus

-

-

34089

-

-

?

chondroitin sulfate A

?

3 entries

chondroitin sulfate A

DELTA4,5-unsaturated chondroitin sulfate A disaccharide

2 entries

chondroitin sulfate A

unsaturated chondroitin sulfate A disaccharides

Vibrio sp. FC509

A0A077ERI8

-

747033

-

-

?

chondroitin sulfate A

unsaturated sulfated chondroitin A disaccharide

Proteus vulgaris

-

-

665450

-

-

?

chondroitin sulfate B

DELTA4,5-unsaturated chondroitin sulfate B disaccharide

2 entries

chondroitin sulfate C

?

3 entries

chondroitin sulfate C

DELTA4,5-unsaturated chondroitin sulfate C disaccharide

2 entries

chondroitin sulfate C

unsaturated chondroitin sulfate C disaccharide

Proteus vulgaris

-

-

665450

-

-

?

chondroitin sulfate C

unsaturated chondroitin sulfate C disaccharides

Vibrio sp. FC509

A0A077ERI8

-

747033

-

-

?

chondroitin sulfate D

?

3 entries

chondroitin sulfate D

unsaturated chondroitin sulfate D disaccharide

Proteus vulgaris

-

-

665450

contains 2-acetamido-2-deoxy-3-O-(P-n-gluco-4-enepyranosyluronic acid)-6-O-sulfo-D-galactose

-

?

chondroitin sulfate D

unsaturated chondroitin sulfate D disaccharides

Vibrio sp. FC509

A0A077ERI8

-

747033

-

-

?

chondroitin sulfate E

?

3 entries

chondroitin sulfate E

unsaturated chondroitin sulfate E disaccharide

Proteus vulgaris

-

-

665450

-

-

?

chondroitin sulfate E

unsaturated chondroitin sulfate E disaccharides

Vibrio sp. FC509

A0A077ERI8

-

747033

-

-

?

chondroitin sulfate proteoglycan

?

3 entries

chondroitin-4-sulfate

2-acetamido-2-deoxy-3-O-(3-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose

2 entries

decorin

?

Proteus vulgaris

-

-

706560

-

-

?

dermatan sulfate

2-acetamido-2-deoxy-3-O-(3-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose + 2-acetamido-2-deoxy-3-O-(3-D-gluco-4-enepyranosyluronic acid)-6-O-sulfo-D-galactose + 2-acetamido-2-deoxy-3-O-(3-D-gluco-4-enepyranosyluronic acid)-4,6-O-bis-sulfo-D-galactose

Proteus vulgaris

-

-

704643

-

-

?

dermatan sulfate

?

4 entries

dermatan sulfate

unsaturated dermatan disaccharides

Vibrio sp. FC509

A0A077ERI8

-

747033

-

-

?

dermatan sulfate

unsaturated dermatan sulfate disaccharide

Pedobacter heparinus

-

-

34082

-

-

?

GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc-beta-2-pyridinaminoside + H2O

GlcA-beta-(1->3)-GalNAc + DELTA4GlcA-beta-(1->3)-GalNAc + GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc-beta-2-pyridinaminoside

Streptococcus dysgalactiae

-

-

655903

-

-

?

GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc-beta-2-pyridinaminoside + H2O

GlcA-beta-(1->3)-GalNAc + DELTA4GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc-beta-2-pyridinaminoside

Streptococcus dysgalactiae

-

-

655903

-

-

?

GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc4S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc4S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc4S-beta-2-pyridinaminoside + 2 H2O

GlcA-beta-(1->3)-GalNAc + DELTA4GlcA-beta-(1->3)-GalNAc + GlcA-beta-(1->3)-GalNAc4S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc4S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc4S-beta-2-pyridinaminoside

Streptococcus dysgalactiae

-

-

655903

-

-

?

GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-2-pyridinaminoside + 2 H2O

GlcA-beta-(1->3)-GalNAc + GlcA-beta-(1->3)-GalNAc + DELTA4GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-2-pyridinaminoside

Streptococcus dysgalactiae

-

-

655903

-

-

?

GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc4S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc4S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc4S-beta-2-pyridinaminoside + H2O

GlcA-beta-(1->3)-GalNAc + DELTA4GlcA-beta-(1->3)-GalNAc4S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc4S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc4S-beta-2-pyridinaminoside

Streptococcus dysgalactiae

-

-

655903

-

-

?

GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc4S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc4S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-2-pyridinaminoside + H2O

GlcA-beta-(1->3)-GalNAc + DELTA4GlcA-beta-(1->3)-GalNAc4S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc4S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-2-pyridinaminoside

Streptococcus dysgalactiae

-

-

655903

-

-

?

GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-2-pyridinaminoside + H2O

GlcA-beta-(1->3)-GalNAc + DELTA4GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-2-pyridinaminoside

Streptococcus dysgalactiae

-

-

655903

-

-

?

GlcA-beta-(1->3)-GalNAc4S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc4S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc-beta-2-pyridinaminoside + 2 H2O

GlcA-beta-(1->3)-GalNAc4S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc4S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc + DELTA4GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc-beta-2-pyridinaminoside

Streptococcus dysgalactiae

-

-

655903

-

-

?

GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc-beta-2-pyridinaminoside + 2 H2O

GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc + DELTA4GlcA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc-beta-2-pyridinaminoside

Streptococcus dysgalactiae

-

-

655903

-

-

?

GlcA-beta-(1->3)-GlcNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-2-pyridinaminoside + H2O

GlcA-beta-(1->3)-GlcNAc + DELTA4GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-2-pyridinaminoside

Streptococcus dysgalactiae

-

-

655903

-

-

?

high molecular weight hyaluronic acid D

?

Bacteroides thetaiotaomicron

Q8A2I1

relative activity: 1.1%

690969

-

-

?

hyaluronan

?

2 entries

hyaluronan

unsaturated hyaluronan disaccharides

Vibrio sp. FC509

A0A077ERI8

-

747033

-

-

?

hyaluronic acid

?

2 entries

IdoA-beta-(1->3)-GalNAc-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-2-pyridinaminoside + H2O

IdoA-beta-(1->3)-GalNAc + DELTA4GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-(1->4)-GlcA-beta-(1->3)-GalNAc6S-beta-2-pyridinaminoside

Streptococcus dysgalactiae

-

-

655903

-

-

?

low molecular weight hyaluronic acid D

?

Bacteroides thetaiotaomicron

Q8A2I1

relative activity: 1.1%

690969

-

-

?

additional information

?

-

7 entries

bovine nasal cartilage proteoglycan

2-acetamido-2-deoxy-3-O-(3-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose + bovine nasal cartilage proteoglycan core

Pedobacter heparinus

-

-

34081

-

-

?

bovine nasal cartilage proteoglycan

2-acetamido-2-deoxy-3-O-(3-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose + bovine nasal cartilage proteoglycan core

Proteus vulgaris

-

-

34081

-

-

?

chondroitin

?

Pedobacter heparinus

-

-

34086

-

-

?

chondroitin

?

Proteus vulgaris

-

-

663950, 704643

-

-

?

chondroitin

?

Proteus vulgaris

-

96% of the rate with chondroitin-6-sulfate

662099

-

-

?

chondroitin

?

Proteus vulgaris

-

reaction is less efficient than for chondroitin sulfates

34086

-

-

?

chondroitin 4-sulfate

?

Bacteroides thetaiotaomicron

Q8A2I1

liquid chromatography-mass spectrometry is applied to determine the action pattern of different chondroitin lyases: products afforded by chondroitinase ABC from Bacteroides thetaiotaomicron and chondroitinase ACII from Arthrobacter aurescens, contain primarily unsatuated disaccharide. These two exolytic enzymes showed different minor products, suggesting some subtle specificity differences between the actions of these two exolytic lyases on chondroitin sulfate A

690418

-

-

?

chondroitin 4-sulfate

?

Paenarthrobacter aurescens

-

liquid chromatography-mass spectrometry is applied to determine the action pattern of different chondroitin lyases: products afforded by chondroitinase ABC from Bacteroides thetaiotaomicron and chondroitinase ACII from Arthrobacter aurescens, contain primarily unsatuated disaccharide. These two exolytic enzymes showed different minor products, suggesting some subtle specificity differences between the actions of these two exolytic lyases on chondroitin sulfate A

690418

-

-

?

chondroitin 4-sulfate

?

Proteus vulgaris

-

83% of the rate with chondroitin-6-sulfate

662099

-

-

?

chondroitin 4-sulfate

unsaturated chondroitin 4-sulfate disaccharide

Pedobacter heparinus

-

-

34082

-

-

?

chondroitin 4-sulfate

unsaturated chondroitin 4-sulfate disaccharide

Proteus vulgaris

-

-

704643

the major product

-

?

chondroitin 6-sulfate

unsaturated chondroitin 6-sulfate disaccharide

Pedobacter heparinus

-

-

34082

-

-

?

chondroitin 6-sulfate

unsaturated chondroitin 6-sulfate disaccharide

Proteus vulgaris

-

-

662099, 663950, 663965

-

-

?

chondroitin sulfate A

?

Bacteroides thetaiotaomicron

-

-

34087

-

-

?

chondroitin sulfate A

?

Bacteroides thetaiotaomicron

Q8A2I1

bovine trachea, relative activity: 100%

690969

-

-

?

chondroitin sulfate A

?

Bacteroides thetaiotaomicron

Q8A2I1

from porcine trachea

692529

-

-

?

chondroitin sulfate A

DELTA4,5-unsaturated chondroitin sulfate A disaccharide

Pedobacter heparinus

-

-

34086

-

-

?

chondroitin sulfate A

DELTA4,5-unsaturated chondroitin sulfate A disaccharide

Proteus vulgaris

-

-

34086

-

-

?

chondroitin sulfate B

DELTA4,5-unsaturated chondroitin sulfate B disaccharide

Proteus vulgaris

-

-

34086

-

-

?

chondroitin sulfate B

DELTA4,5-unsaturated chondroitin sulfate B disaccharide

Proteus vulgaris

-

-

665450

contains 2-acetamido-2-deoxy-3-O-(P-n-gluco-4-enepyranosyluronic acid)-4-O-sulfo-n-galactose

-

?

chondroitin sulfate C

?

Bacteroides thetaiotaomicron

-

-

34087

-

-

?

chondroitin sulfate C

?

Bacteroides thetaiotaomicron

Q8A2I1

from shark cartilage

692529

-

-

?

chondroitin sulfate C

?

Bacteroides thetaiotaomicron

Q8A2I1

shark cartilage, relative activity: 61.1%

690969

-

-

?

chondroitin sulfate C

DELTA4,5-unsaturated chondroitin sulfate C disaccharide

Pedobacter heparinus

-

-

34086

-

-

?

chondroitin sulfate C

DELTA4,5-unsaturated chondroitin sulfate C disaccharide

Proteus vulgaris

-

-

34086

-

-

?

chondroitin sulfate D

?

Bacteroides thetaiotaomicron

Q8A2I1

skate cartilage, relative activity: 18.5%

690969

-

-

?

chondroitin sulfate D

?

Proteus vulgaris

-

-

663950, 704643

-

-

?

chondroitin sulfate D

?

Proteus vulgaris

-

93% of the rate with chondroitin-6-sulfate

662099

-

-

?

chondroitin sulfate E

?

Bacteroides thetaiotaomicron

Q8A2I1

skate cartilage, relative activity: 36.7%

690969

-

-

?

chondroitin sulfate E

?

Proteus vulgaris

-

-

663950, 704643

-

-

?

chondroitin sulfate E

?

Proteus vulgaris

-

90% of the rate with chondroitin-6-sulfate

662099

-

-

?

chondroitin sulfate proteoglycan

?

Proteus vulgaris

-

67% of the rate with chondroitin-6-sulfate

662099

-

-

?

chondroitin sulfate proteoglycan

?

Proteus vulgaris

-

ChABC digests the glycosaminoglycan side chain of chondroitin sulfate proteoglycan

705924, 706560

-

-

?

chondroitin sulfate proteoglycan

?

Proteus vulgaris

-

ChABC digests the glycosaminoglycan side chain of chondroitin sulfate proteoglycan to promote axonal re-growth across the injured site

705923

-

-

?

chondroitin-4-sulfate

2-acetamido-2-deoxy-3-O-(3-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose

Pedobacter heparinus

-

-

34081

-

-

?

chondroitin-4-sulfate

2-acetamido-2-deoxy-3-O-(3-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose

Proteus vulgaris

-

-

34081

-

-

?

dermatan sulfate

?

Bacteroides thetaiotaomicron

Q8A2I1

from porcine intestinal mucosa

692529

-

-

?

dermatan sulfate

?

Bacteroides thetaiotaomicron

Q8A2I1

porcine intestinal mucosa, relative activity: 9.1%

690969

-

-

?

dermatan sulfate

?

Proteus vulgaris

-

-

663950, 663965

-

-

?

dermatan sulfate

?

Proteus vulgaris

-

77% of the rate with chondroitin-6-sulfate

662099

-

-

?

hyaluronan

?

Proteus vulgaris

-

-

663950

-

-

?

hyaluronan

?

Proteus vulgaris

-

17% of the rate with chondroitin-6-sulfate

662099

-

-

?

hyaluronic acid

?

Pedobacter heparinus

-

-

34086

-

-

?

hyaluronic acid

?

Proteus vulgaris

-

bad substrate

34086

-

-

?

additional information

?

-

Pedobacter heparinus

-

no substrate: chondroitin sulfate B, keratosulfate, heparin, or heparitin sulfate

34086

-

-

?

additional information

?

-

Proteus vulgaris

-

no substrate: keratan sulfate, heparin, heparan sulfate. Activity of enzyme increases with decreasing of chain size from poly-, hexa-, to tetrasaccharide substrates

662099

-

-

?

additional information

?

-

Proteus vulgaris

-

no substrate: keratosulfate, heparin, or heparitin sulfate

34086

-

-

?

additional information

?

-

Proteus vulgaris

-

cABCII is an exolytic enzyme that cleaves the substrate only at the nonreducing end of the polysaccharide

704643

-

-

?

additional information

?

-

Proteus vulgaris

-

the recombinant protein with the sequence discrepancies is unable to effectively process galactosaminoglycan substrates, the enzyme does not react with hyaluronan, heparin/heparan sulfate, and keratan sulfate

704643

-

-

?

additional information

?

-

Proteus vulgaris NCTC 4636

-

no substrate: keratosulfate, heparin, or heparitin sulfate

34086

-

-

?

additional information

?

-

Vibrio sp. FC509

A0A077ERI8

no substrate: 2-aminobenzamide-labeled hyaluronan oligosaccharides, 2-aminobenzamide-labeled unsulfated chondroitin oligisaccharides, 2-aminobenzamide-labeled dermatan sulfate oligisaccharides

747033

-

-

?