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Literature summary for 4.2.2.21 extracted from

  • Hamai, A.; Hashimoto, N.; Mochizuki, H.; Kato, F.; Makiguchi, Y.; Horie, K.; Suzuki, S.
    Two distinct chondroitin sulfate ABC lyases. An endoeliminase yielding tetrasaccharides and an exoeliminase preferentially acting on oligosaccharides (1997), J. Biol. Chem., 272, 9123-9130.
    View publication on PubMed
Search for more literature for 4.2.2.21

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: Ni2+, Fe2+, Cu2+, Ca2+, Mg2+, Ba2+, Mn2+ Proteus vulgaris
Zn2+ 1 mM, complete inhibition Proteus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 chondroitin-6-sulfate pH 8.0, 37°C Proteus vulgaris

Organism

Organism UniProt Comment Textmining
Proteus vulgaris
-
 NCTC 4636
-
Proteus vulgaris
-
 NCTC 4636, induced by chondroitin sulfate, isoenzyme ChS ABC lyase II
-

Purification (Commentary)

Purification (Comment) Organism
-
 Proteus vulgaris

Reaction

Reaction Comment Organism Reaction ID
[GlcAbeta(1-3)GalNAc(4S)beta(1-4)]3 = [GlcAbeta(1-3)GalNAc(4S)beta(1-4)]2 + 2-(acetylamino)-2-deoxy-3-O-alpha-L-threo-hex-4-enopyranuronosyl-4-O-sulfo-beta-D-galactopyranose related enzyme EC 4.2.2.20 Proteus vulgaris
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
34
-
 pH 8.0, 37°C Proteus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin 96% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
chondroitin 4-sulfate 83% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
chondroitin 6-sulfate
-
 Proteus vulgaris unsaturated chondroitin 6-sulfate disaccharide
-
 ?
chondroitin sulfate D 93% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
chondroitin sulfate E 90% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
chondroitin sulfate proteoglycan 67% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
dermatan sulfate 77% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
hyaluronan 17% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
additional information no substrate: keratan sulfate, heparin, heparan sulfate. Activity of enzyme increases with decreasing of chain size from poly-, hexa-, to tetrasaccharide substrates Proteus vulgaris ?
-
 ?

Synonyms

Synonyms Comment Organism
ChS ABC lyase II
-
 Proteus vulgaris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Proteus vulgaris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 30 min, 23% loss of activity Proteus vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Proteus vulgaris

pI Value

Organism Comment pI Value Maximum pI Value
Proteus vulgaris and 8.25, isoelectric focusing
-
 8
Proteus vulgaris and 8.0, isoelectric focusing
-
 8.25