4.2.2.21: chondroitin-sulfate-ABC exolyase

This is an abbreviated version!
For detailed information about chondroitin-sulfate-ABC exolyase, go to the full flat file.

Word Map on EC 4.2.2.21

4.2.2.21

glycosaminoglycans

articular

dermatan

tensile

4-sulfated

chabc

neocartilage

fibrocartilage

perineuronal

chondroitinase-ac

oversulfated

l-iduronic

neurocan

analysis

medicine

galactosaminoglycans

chondroitin-4-sulfate

synthesis

biotechnology

Reaction

2-(acetylamino)-2-deoxy-3-O-alpha-L-threo-hex-4-enopyranuronosyl-4-O-sulfo-beta-D-galactopyranose

Synonyms

BactnABC, cABCII, chABC, Chondroitin Sulfate Lyase ABC, chondroitinase ABC, chondroitinase ABC II, chondroitinase AC, chondroitinase ACII, chondroitinase-ABC, ChS ABC lyase II, EC 4.2.2.4, EC 4.2.99.6, HCLase

ECTree

4 Lyases

4.2 Carbon-oxygen lyases

4.2.2 Acting on polysaccharides

4.2.2.21 chondroitin-sulfate-ABC exolyase

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Results	in table
3	Activating Compound
20	AA Sequence
9	Application
4	Cloned(Commentary)
1	Crystallization (Commentary)
2	General Stability
16	Inhibitors
7	kcat/KM [mM/s]
56	KM Value [mM]
1	Localization
8	Metals/Ions
11	Molecular Weight [Da]
31	Organism
4	Pathway
2	PDB ID
17	pH Optimum
2	pH Range
5	pI Value
45	Protein Variants
8	Purification (Commentary)
3	Reaction
28	Reference
1	Source Tissue
21	Specific Activity [micromol/min/mg]
2	Storage Stability
88	Substrates and Products (Substrate)
7	Subunits
22	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
2	Temperature Range [°C]
4	Temperature Stability [°C]
55	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 4.2.2.21 - chondroitin-sulfate-ABC exolyase

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100000

Proteus vulgaris

SDS-PAGE

704643

104000

Bacteroides thetaiotaomicron

x * 108000, isoenzyme I, x * 104000, isoenzyme II, SDS-PAGE

34087

108000

Bacteroides thetaiotaomicron

x * 108000, isoenzyme I, x * 104000, isoenzyme II, SDS-PAGE

34087

110000

Proteus vulgaris

x * 110000, SDS-PAGE, x * 112614, calculated

663950

112614

Proteus vulgaris

x * 110000, SDS-PAGE, x * 112614, calculated

120000

gel filtration

150000

32000

1 * 86000 + 1 * 32000, SDS-PAGE

34085

86000

Proteus vulgaris

1 * 86000 + 1 * 32000, SDS-PAGE

34085

97000 - 116000

Proteus vulgaris

chABC alone migrates between 97 kDa and 116 kDa, SDS-PAGE

706560