Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Proteus vulgaris |
Protein Variants | Comment | Organism |
---|---|---|
E608A | the mutant does not show detectable activity on either chondroitin 6-sulfate or dermatan sulfate | Proteus vulgaris |
H452A | the mutant shows similar Km values to the wild type enzyme but a 100fold reduced catalytic efficiency | Proteus vulgaris |
H453A | the mutant does not show detectable activity on either chondroitin 6-sulfate or dermatan sulfate | Proteus vulgaris |
H456A | the mutant shows about 3fold reduction in catalytic efficiency | Proteus vulgaris |
R513A | the mutant does not show detectable activity on either chondroitin 6-sulfate or dermatan sulfate | Proteus vulgaris |
Y460A | the mutant does not show detectable activity on either chondroitin 6-sulfate or dermatan sulfate | Proteus vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0076 | - |
dermatan sulfate | mutant enzyme H456A, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris | |
0.0077 | - |
chondroitin 6-sulfate | mutant enzyme H452A, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris | |
0.0091 | - |
dermatan sulfate | mutant enzyme H452A, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris | |
0.0098 | - |
chondroitin 6-sulfate | wild type enzyme, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris | |
0.0104 | - |
chondroitin 6-sulfate | mutant enzyme H456A, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris | |
0.0161 | - |
chondroitin 4-sulfate | wild type enzyme, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris | |
0.0192 | - |
dermatan sulfate | wild type enzyme, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
SDS-PAGE | Proteus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Proteus vulgaris | - |
strain ATCC 6896 | - |
Purification (Comment) | Organism |
---|---|
Hi-Trap Ni-chelate column chromatography, gel filtration | Proteus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chondroitin | - |
Proteus vulgaris | ? | - |
? | |
chondroitin 4-sulfate | - |
Proteus vulgaris | unsaturated chondroitin 4-sulfate disaccharide | the major product | ? | |
chondroitin 6-sulfate | - |
Proteus vulgaris | ? | - |
? | |
chondroitin sulfate D | - |
Proteus vulgaris | ? | - |
? | |
chondroitin sulfate E | - |
Proteus vulgaris | ? | - |
? | |
dermatan sulfate | - |
Proteus vulgaris | 2-acetamido-2-deoxy-3-O-(3-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose + 2-acetamido-2-deoxy-3-O-(3-D-gluco-4-enepyranosyluronic acid)-6-O-sulfo-D-galactose + 2-acetamido-2-deoxy-3-O-(3-D-gluco-4-enepyranosyluronic acid)-4,6-O-bis-sulfo-D-galactose | - |
? | |
additional information | cABCII is an exolytic enzyme that cleaves the substrate only at the nonreducing end of the polysaccharide | Proteus vulgaris | ? | - |
? | |
additional information | the recombinant protein with the sequence discrepancies is unable to effectively process galactosaminoglycan substrates, the enzyme does not react with hyaluronan, heparin/heparan sulfate, and keratan sulfate | Proteus vulgaris | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cABCII | isoform | Proteus vulgaris |
chondroitinase ABC II | - |
Proteus vulgaris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
dermatan sulfate | mutant enzyme H452A, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris | |
0.17 | - |
chondroitin 6-sulfate | mutant enzyme H452A, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris | |
2.4 | - |
dermatan sulfate | mutant enzyme H456A, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris | |
7.13 | - |
chondroitin 6-sulfate | mutant enzyme H456A, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris | |
16.67 | - |
chondroitin 4-sulfate | wild type enzyme, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris | |
19.17 | - |
dermatan sulfate | wild type enzyme, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris | |
21.67 | - |
chondroitin 6-sulfate | wild type enzyme, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.3 | - |
dermatan sulfate | mutant enzyme H452A, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris | |
23.3 | - |
chondroitin 6-sulfate | mutant enzyme H452A, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris | |
316.7 | - |
dermatan sulfate | mutant enzyme H456A, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris | |
683.3 | - |
chondroitin 6-sulfate | mutant enzyme H456A, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris | |
1000 | - |
dermatan sulfate | wild type enzyme, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris | |
1000 | - |
chondroitin 4-sulfate | wild type enzyme, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris | |
2200 | - |
chondroitin 6-sulfate | wild type enzyme, in 50mM Tris/HCl, pH 8.0, at 37°C | Proteus vulgaris |