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4.2.2.21: chondroitin-sulfate-ABC exolyase

This is an abbreviated version!
For detailed information about chondroitin-sulfate-ABC exolyase, go to the full flat file.

Word Map on EC 4.2.2.21

Reaction

[GlcAbeta(1-3)GalNAc(4S)beta(1-4)]3
=
[GlcAbeta(1-3)GalNAc(4S)beta(1-4)]2
 +
2-(acetylamino)-2-deoxy-3-O-alpha-L-threo-hex-4-enopyranuronosyl-4-O-sulfo-beta-D-galactopyranose

Synonyms

BactnABC, cABCII, chABC, Chondroitin Sulfate Lyase ABC, chondroitinase ABC, chondroitinase ABC II, chondroitinase AC, chondroitinase ACII, chondroitinase-ABC, ChS ABC lyase II, EC 4.2.2.4, EC 4.2.99.6, HCLase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.21 chondroitin-sulfate-ABC exolyase

Engineering

Engineering on EC 4.2.2.21 - chondroitin-sulfate-ABC exolyase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E628D
mutant shows no activity
E628Q
Km (mM) (chondroitin sulfate A) 0.158, (chondroitin sulfate C) 0.035, kcat (1/sec) (chondroitin sulfate A) 10.38, (chondroitin sulfate B) 4.41, no activity toward dermatan sulfate
H344A
Km (mM) (chondroitin sulfate A) 0.202, (chondroitin sulfate C) 0.134, kcat (1/sec) (chondroitin sulfate A) 92.66, (chondroitin sulfate B) 35.71, no activity toward dermatan sulfate
H344D
mutant shows no activity
H344E
mutant shows no activity
H344N
Km (mM) (chondroitin sulfate A) 0.039, (chondroitin sulfate C) 0.084, (dermatan sulfate) 0.09, kcat (1/sec) (chondroitin sulfate A) 42.2, (chondroitin sulfate B) 18.7, (dermatan sulfate) 9.06
H344Q
Km (mM) (chondroitin sulfate A) 0.073, kcat (1/sec) (chondroitin sulfate A) 16.78, no activity toward dermatan sulfate or chondroitin sulfate C
H345A
Km (mM) (chondroitin sulfate A) 0.171, (chondroitin sulfate C) 0.195, kcat (1/sec) (chondroitin sulfate A) 63, (chondroitin sulfate B) 30.88, no activity toward dermatan sulfate
H345D/E
mutant shows no activity
H345N
Km (mM) (chondroitin sulfate A) 0.061, (chondroitin sulfate C) 0.026, kcat (1/sec) (chondroitin sulfate A) 21, (chondroitin sulfate B) 6.6, no activity toward dermatan sulfate
H345Q
Km (mM) (chondroitin sulfate A) 0.045, (chondroitin sulfate C) 0.102, kcat (1/sec) (chondroitin sulfate A) 23.46, (chondroitin sulfate B) 10.85, no activity toward dermatan sulfate
H453A
Km (mM) (chondroitin sulfate A) 0.038, (chondroitin sulfate C) 0.02, (dermatan sulfate) 0.044, kcat (1/sec) (chondroitin sulfate A) 17, (chondroitin sulfate B) 6.41, (dermatan sulfate) 5.8
H453N
Km (mM) (chondroitin sulfate A) 0.034, (chondroitin sulfate C) 0.012, kcat (1/sec) (chondroitin sulfate A) 13.15, (chondroitin sulfate B) 6.8, no activity toward dermatan sulfate
H454/D/N/Q
mutant shows no activity
H454A
mutant shows no activity
Q173A
Km (mM) (chondroitin sulfate A) 0.102, (chondroitin sulfate C) 0.050, (dermatan sulfate) 0.071, kcat (1/sec) (chondroitin sulfate A) 123, (chondroitin sulfate B) 33, (dermatan sulfate) 6.8
R172A
mutant shows no activity
R267A
Km (mM) (chondroitin sulfate A) 0.109, (chondroitin sulfate C) 0.098, (dermatan sulfate) 0.071, kcat (1/sec) (chondroitin sulfate A) 205, (chondroitin sulfate B) 61.7, (dermatan sulfate) 4.1
R514A
mutant shows no activity
Y461A
mutant shows no activity
Y461F
mutant shows no activity
E608A
-
the mutant does not show detectable activity on either chondroitin 6-sulfate or dermatan sulfate
E653A
-
no enzymic activity
E653D
-
no enzymic activity
E653Q
-
5fold increase in Km-value
H452A
-
the mutant shows similar Km values to the wild type enzyme but a 100fold reduced catalytic efficiency
H453A
-
the mutant does not show detectable activity on either chondroitin 6-sulfate or dermatan sulfate
H456A
-
the mutant shows about 3fold reduction in catalytic efficiency
H501A
-
no enzymic activity
H501K
-
no enzymic activity
H501R
-
no enzymic activity
H561A
-
increase in Km-values
H712A
-
increase in Km-values
R500A
R513A
-
the mutant does not show detectable activity on either chondroitin 6-sulfate or dermatan sulfate
R560A
-
no enzymic activity
Y460A
-
the mutant does not show detectable activity on either chondroitin 6-sulfate or dermatan sulfate
Y508A
-
no enzymic activity
Y508F
-
30fold increase in Km-value