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Literature summary for 4.2.2.21 extracted from

  • Prabhakar, V.; Raman, R.; Capila, I.; Bosques, C.J.; Pojasek, K.; Sasisekharan, R.
    Biochemical characterization of the chondroitinase ABC I active site (2005), Biochem. J., 390, 395-405.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.21

Protein Variants

Protein Variants Comment Organism
E653A no enzymic activity Proteus vulgaris
E653D no enzymic activity Proteus vulgaris
E653Q 5fold increase in Km-value Proteus vulgaris
H501A no enzymic activity Proteus vulgaris
H501K no enzymic activity Proteus vulgaris
H501R no enzymic activity Proteus vulgaris
R500A 20fold increase in Km-value Proteus vulgaris
R560A no enzymic activity Proteus vulgaris
Y508A no enzymic activity Proteus vulgaris
Y508F 30fold increase in Km-value Proteus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 chondroitin 6-sulfate wild-type, pH 8.0, 37°C Proteus vulgaris
0.0025
-
 dermatan sulfate wild-type, pH 8.0, 37°C Proteus vulgaris
0.0042
-
 dermatan sulfate mutant E653Q, pH 8.0, 37°C Proteus vulgaris
0.0061
-
 chondroitin 6-sulfate mutant E653Q, pH 8.0, 37°C Proteus vulgaris
0.02
-
 chondroitin 6-sulfate mutant R500A, pH 8.0, 37°C Proteus vulgaris
0.036
-
 dermatan sulfate mutant R500A, pH 8.0, 37°C Proteus vulgaris
0.036
-
 chondroitin 6-sulfate mutant Y508F, pH 8.0, 37°C Proteus vulgaris
0.049
-
 dermatan sulfate mutant Y508F, pH 8.0, 37°C Proteus vulgaris

Organism

Organism UniProt Comment Textmining
Proteus vulgaris
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin 6-sulfate
-
 Proteus vulgaris unsaturated chondroitin 6-sulfate disaccharide
-
 ?
dermatan sulfate
-
 Proteus vulgaris ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 chondroitin 6-sulfate mutant Y508F, pH 8.0, 37°C Proteus vulgaris
1.8
-
 dermatan sulfate mutant Y508F, pH 8.0, 37°C Proteus vulgaris
2.7
-
 dermatan sulfate mutant R500A, pH 8.0, 37°C Proteus vulgaris
6.8
-
 chondroitin 6-sulfate mutant R500A, pH 8.0, 37°C Proteus vulgaris
26.7
-
 chondroitin 6-sulfate mutant E653Q, pH 8.0, 37°C Proteus vulgaris
87
-
 dermatan sulfate mutant E653Q, pH 8.0, 37°C Proteus vulgaris
450
-
 dermatan sulfate wild-type, pH 8.0, 37°C Proteus vulgaris
617
-
 chondroitin 6-sulfate wild-type, pH 8.0, 37°C Proteus vulgaris