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Literature summary for 4.2.2.21 extracted from

  • Wang, W.; Cai, X.; Han, N.; Han, W.; Sugahara, K.; Li, F.
    Sequencing of chondroitin sulfate oligosaccharides using a novel exolyase from a marine bacterium that degrades hyaluronan and chondroitin sulfate/dermatan sulfate (2017), Biochem. J., 474, 3831-3848 .
    View publication on PubMed
Search for more literature for 4.2.2.21

Application

Application Comment Organism
analysis enzyme combined with chodroitin sulfatase ABC, EC 4.2.2.4, can be applied for the sequencing of chondroitin sulfate hexa- and octasaccharides with complex structures Vibrio sp. FC509

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Vibrio sp. FC509

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble recombinant protein Vibrio sp. FC509
-
-

Organism

Organism UniProt Comment Textmining
Vibrio sp. FC509 A0A077ERI8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Vibrio sp. FC509

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
750
-
 substrate dermatan sulfate, pH 7.0, 30°C Vibrio sp. FC509
800
-
 substrate hyaluronan, pH 7.0, 30°C Vibrio sp. FC509
1210
-
 substrate chondroitin sulfate C, pH 7.0, 30°C Vibrio sp. FC509
1230
-
 substrate chondroitin sulfate A, pH 7.0, 30°C Vibrio sp. FC509
1350
-
 substrate chondroitin sulfate D, pH 7.0, 30°C Vibrio sp. FC509
1480
-
 substrate chondroitin sulfate E, pH 7.0, 30°C Vibrio sp. FC509

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzamide-labeled chondroitin sulfate-C hexasaccharide
-
 Vibrio sp. FC509 unsaturated 2-aminobenzamide-labeled chondroitin sulfate-C disaccharide + ?
-
 ?
2-aminobenzamide-labeled chondroitin sulfate-C tetrasaccharide
-
 Vibrio sp. FC509 unsaturated 2-aminobenzamide-labeled chondroitin sulfate-C disaccharide + disaccharide
-
 ?
chondroitin sulfate A
-
 Vibrio sp. FC509 unsaturated chondroitin sulfate A disaccharides
-
 ?
chondroitin sulfate C
-
 Vibrio sp. FC509 unsaturated chondroitin sulfate C disaccharides
-
 ?
chondroitin sulfate D
-
 Vibrio sp. FC509 unsaturated chondroitin sulfate D disaccharides
-
 ?
chondroitin sulfate E
-
 Vibrio sp. FC509 unsaturated chondroitin sulfate E disaccharides
-
 ?
dermatan sulfate
-
 Vibrio sp. FC509 unsaturated dermatan disaccharides
-
 ?
hyaluronan
-
 Vibrio sp. FC509 unsaturated hyaluronan disaccharides
-
 ?
additional information no substrate: 2-aminobenzamide-labeled hyaluronan oligosaccharides, 2-aminobenzamide-labeled unsulfated chondroitin oligisaccharides, 2-aminobenzamide-labeled dermatan sulfate oligisaccharides Vibrio sp. FC509 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 114700, calculated from sequence, x * 115000, SDS-PAGE Vibrio sp. FC509

Synonyms

Synonyms Comment Organism
HCLase
-
 Vibrio sp. FC509

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Vibrio sp. FC509

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Vibrio sp. FC509

pH Range

pH Minimum pH Maximum Comment Organism
6
-
 about 60% of maximum activity Vibrio sp. FC509

pI Value

Organism Comment pI Value Maximum pI Value
Vibrio sp. FC509 calculated from sequence
-
 5.2