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Literature summary for 4.2.2.21 extracted from

  • Zhang, Z.; Park, Y.; Kemp, M.M.; Zhao, W.; Im, A.R.; Shaya, D.; Cygler, M.; Kim, Y.S.; Linhardt, R.J.
    Liquid chromatography-mass spectrometry to study chondroitin lyase action pattern (2009), Anal. Biochem., 385, 57-64.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Bacteroides thetaiotaomicron

Organism

Organism UniProt Comment Textmining
Bacteroides thetaiotaomicron Q8A2I1
-
-
Paenarthrobacter aurescens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin 4-sulfate liquid chromatography-mass spectrometry is applied to determine the action pattern of different chondroitin lyases: products afforded by chondroitinase ABC from Bacteroides thetaiotaomicron and chondroitinase ACII from Arthrobacter aurescens, contain primarily unsatuated disaccharide. These two exolytic enzymes showed different minor products, suggesting some subtle specificity differences between the actions of these two exolytic lyases on chondroitin sulfate A Paenarthrobacter aurescens ?
-
 ?
chondroitin 4-sulfate liquid chromatography-mass spectrometry is applied to determine the action pattern of different chondroitin lyases: products afforded by chondroitinase ABC from Bacteroides thetaiotaomicron and chondroitinase ACII from Arthrobacter aurescens, contain primarily unsatuated disaccharide. These two exolytic enzymes showed different minor products, suggesting some subtle specificity differences between the actions of these two exolytic lyases on chondroitin sulfate A Bacteroides thetaiotaomicron ?
-
 ?

Synonyms

Synonyms Comment Organism
chondroitinase ABC
-
 Bacteroides thetaiotaomicron
chondroitinase ACII
-
 Paenarthrobacter aurescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Paenarthrobacter aurescens
37
-
 assay at Bacteroides thetaiotaomicron

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Paenarthrobacter aurescens
7
-
 assay at Bacteroides thetaiotaomicron