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4.1.1.15: glutamate decarboxylase

This is an abbreviated version!
For detailed information about glutamate decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.15

Reaction

L-glutamate
=
4-aminobutanoate
 +
CO2

Synonyms

42 kDa biotin-coupled protein, 65 kDa glutamic acid decarboxylase, 67 kDa glutamic acid decarboxylase, Aspartate 1-decarboxylase, Aspartic alpha-decarboxylase, AtGAD1, BmGAD, BmGadB, BMI_II334, Cysteic acid decarboxylase, Decarboxylase, glutamate, ERT D1, GAD, GAD B, GAD-65, GAD-67, GAD-alpha, GAD-beta, GAD-gamma, GAD1, GAD2, GAD3, Gad56, GAD65, GAD67, GadA, GadB, GADB1, gadB2, GADbeta, GADCase, gadlbhye1, gamma-Glutamate decarboxylase, GDC, GDCase, Glt decarboxylase, glutamate decarboxylase, glutamate decarboxylase 1, glutamate decarboxylase 65, glutamate decarboxylase 67, glutamate decarboxylase A, glutamate decarboxylase B, Glutamic acid decarboxylase, glutamic acid decarboxylase 65, glutamic acid decarboxylase 67, glutamic acid decarboxylase-65, glutamic acid decarboxylase-67, glutamic acid decarboxylase67, Glutamic decarboxylase, hGAD65, hGAD67, L-Aspartate-alpha-decarboxylase, L-glutamate 1-carboxylyase, L-Glutamate alpha-decarboxylase, L-Glutamate decarboxylase, L-glutamate decarboxylase 65, L-glutamate decarboxylase 67, L-Glutamic acid decarboxylase, L-Glutamic decarboxylase, LbGAD, LbGadB, MGAD, More, p42, PF1159, PfGAD, ScGAD, sll1641

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.15 glutamate decarboxylase

Specific Activity

Specific Activity on EC 4.1.1.15 - glutamate decarboxylase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00024
recombinant GST-tagged enzyme, pH 8.0, 37°C
0.00038
recombinant MBP-tagged enzyme, pH 8.0, 37°C
0.0011
-
purified native enzyme
0.009
-
crude extract, in 50 mM PBS buffer, pH 5.6, at 40°C
0.039
-
mutant with N-terminal deletion of 70 amino acids, pH 7.2
0.041
-
mutant with N-terminal deletion of 90 amino acids, pH 7.2
0.216
-
wild-type, pH 7.2
0.462
-
-
1.37
-
-
1.85
-
GAD67
1.96
-
GAD65
103.5
130.2
purified recombinant GAD obtained without supplementation (GAD-C), pH 4.8, 30°C
148
purified recombinant wild-type enzyme, pH 5.0, 50°C
15
wild-type, pH 7.0, 30°C, absence of Ca2+/calmodulin
16.7
purified recombinant enzyme
17.3
wild-type, pH 7.0, 30°C, presence of Ca2+/calmodulin
193.4
purified recombinant GAD produced in media supplemented with 0.05 mM soluble vitamin B6 analogue pyridoxine hydrochloride (GAD-V), pH 4.8, 30°C
197
wild-type, pH 6.0, 30°C, presence of Ca2+/calmodulin
199
wild-type, pH 6.0, 30°C, absence of Ca2+/calmodulin
2.956
-
-
21.44
purified native enzyme, pH 6.0, 40°C
21.7
-
purified native enzyme, pH 4.6, 45°C
223.4
-
pH 5.6, 40°C
2719
mutant lacking 31 C-terminal amino acids, pH 6.0, 30°C, presence of Ca2+/calmodulin
2775
mutant lacking 31 C-terminal amino acids, pH 6.0, 30°C, absence of Ca2+/calmodulin
3.25
-
-
4670
mutant lacking 31 C-terminal amino acids, pH 5.0, 30°C, absence of Ca2+/calmodulin
4850
mutant lacking 31 C-terminal amino acids, pH 5.0, 30°C, presence of Ca2+/calmodulin
59
purified recombinant wild-type enzyme, pH 6.0, 50°C
605
mutant lacking 31 C-terminal amino acids, pH 7.0, 30°C, presence of Ca2+/calmodulin
641
mutant lacking 31 C-terminal amino acids, pH 7.0, 30°C, absence of Ca2+/calmodulin
685
wild-type, pH 5.0, 30°C, absence of Ca2+/calmodulin
7.666
-
after 851.7fold purification, in 50 mM PBS buffer, pH 5.6, at 40°C
71 - 86
-
-
710
wild-type, pH 5.0, 30°C, presence of Ca2+/calmodulin
additional information