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4.1.1.15: glutamate decarboxylase

This is an abbreviated version!
For detailed information about glutamate decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.15

Reaction

L-glutamate
=
4-aminobutanoate
 +
CO2

Synonyms

42 kDa biotin-coupled protein, 65 kDa glutamic acid decarboxylase, 67 kDa glutamic acid decarboxylase, Aspartate 1-decarboxylase, Aspartic alpha-decarboxylase, AtGAD1, BmGAD, BmGadB, BMI_II334, Cysteic acid decarboxylase, Decarboxylase, glutamate, ERT D1, GAD, GAD B, GAD-65, GAD-67, GAD-alpha, GAD-beta, GAD-gamma, GAD1, GAD2, GAD3, Gad56, GAD65, GAD67, GadA, GadB, GADB1, gadB2, GADbeta, GADCase, gadlbhye1, gamma-Glutamate decarboxylase, GDC, GDCase, Glt decarboxylase, glutamate decarboxylase, glutamate decarboxylase 1, glutamate decarboxylase 65, glutamate decarboxylase 67, glutamate decarboxylase A, glutamate decarboxylase B, Glutamic acid decarboxylase, glutamic acid decarboxylase 65, glutamic acid decarboxylase 67, glutamic acid decarboxylase-65, glutamic acid decarboxylase-67, glutamic acid decarboxylase67, Glutamic decarboxylase, hGAD65, hGAD67, L-Aspartate-alpha-decarboxylase, L-glutamate 1-carboxylyase, L-Glutamate alpha-decarboxylase, L-Glutamate decarboxylase, L-glutamate decarboxylase 65, L-glutamate decarboxylase 67, L-Glutamic acid decarboxylase, L-Glutamic decarboxylase, LbGAD, LbGadB, MGAD, More, p42, PF1159, PfGAD, ScGAD, sll1641

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.15 glutamate decarboxylase

Temperature Stability

Temperature Stability on EC 4.1.1.15 - glutamate decarboxylase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 50
-
1 h, stable in this range
20 - 40
purified recombinant enzyme, stable
25
-
pH 7.0, 2 h, stable
30 - 40
-
purified native enzyme, pH 4.6, over 95% activity remaining after 5 h
50 - 60
after 12 h incubation at 50°C and 60°C, the residual activity of wild-type GadB is 12.5% and 9.0%, respectively. The residual activity of mutants M1, M6 and M8 is 35.16% and 27.17%, 38.10% and 33.25%, 27.19% and 24.39%, respectively, which is at least 2fold higher than that of wild-type GadB
51
-
transition temperature of wild-type enzyme
56
truncated enzyme mutant AtGAD1-DELTA1-24, T50 value
60 - 70
the enzyme is significantly inactivated when the temperature is increased to 60°C, with 36 and 49% of the original activity being lost after 30 and 60 min of incubation respectively, and is completely inactivated at 70°C
62
-
transition temperature of mutant enzyme K276A
65
the recombinant immobilized enzyme retains about 70% of its initial activity after incubation for 1 h, while the recombinant free enzyme loses all activity
70 - 90
the recombinant enzyme with a thermal half-life of 46 min at 90°C retains approximately 70% of initial activity after incubation at 70°C for 9 h, whereas the enzyme is rapidly inactivated at 100°C
75.18
Tm of purified recombinant His-tagged enzyme
85
-
purified recombinant enzyme, stable
95
-
purified recombinant enzyme, half-life is 3 h
additional information