Cloned (Comment) | Organism |
---|---|
gene gad, cloning from germinated faba bean, DNA and amino acid sequence determination and analysis, sequence comparisons | Vicia faba |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Vicia faba | |
Aminoxyacetate | - |
Vicia faba | |
Ba2+ | - |
Vicia faba | |
Ca2+ | activates at 0.2 mM, slight inhibition above 1 mM | Vicia faba | |
Cu2+ | strong inhibition at 3 mM | Vicia faba | |
EGTA | - |
Vicia faba | |
Fe3+ | - |
Vicia faba | |
L-cysteine | - |
Vicia faba | |
Mg2+ | - |
Vicia faba | |
Na2EDTA | - |
Vicia faba |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.63 | - |
L-glutamate | pH 6.0, 40°C, recombinant enzyme | Vicia faba |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates at 0.2 mM, slight inhibition above 1 mM | Vicia faba |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57740 | - |
x * 57740, sequence calculation, x * 58000, SDS-PAGE | Vicia faba |
58000 | - |
x * 57740, sequence calculation, x * 58000, SDS-PAGE | Vicia faba |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | Vicia faba | - |
4-aminobutanoate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vicia faba | M1GCP8 | cv. Qidou 2 | - |
Purification (Comment) | Organism |
---|---|
native enzyme 32fold by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography | Vicia faba |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.44 | - |
purified native enzyme, pH 6.0, 40°C | Vicia faba |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Vicia faba | 4-aminobutanoate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57740, sequence calculation, x * 58000, SDS-PAGE | Vicia faba |
Synonyms | Comment | Organism |
---|---|---|
GAD | - |
Vicia faba |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant enzyme | Vicia faba |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | activity range, profile overview, inactivation above | Vicia faba |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
recombinant enzyme | Vicia faba |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | activity range, profile overview | Vicia faba |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Vicia faba | sequence calculation | - |
5.41 |