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3.4.22.34: Legumain

This is an abbreviated version!
For detailed information about Legumain, go to the full flat file.

Word Map on EC 3.4.22.34

Reaction

hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds =

Synonyms

AEP, Antigen Sj32, Antigen SM32, As-specific vacuolar cysteine proteinase, Asn-specific vacuolar cysteine proteinase, asparagine endopeptidase, asparaginly endopeptidase, asparaginyl endopepidase, Asparaginyl endopeptidase, asparaginyl endopeptidase legumain, asparaginyl endopeptidase,, asparaginyl proteinase, asparaginyl-specific cysteine endopeptidase, asparagyl-endopeptidase, aspartate-specific endopeptidase, AtLEGgamma, Bean endopeptidase, C197 legumain, c197 Sm32, cysteine protease 1, hemoglobinase, HlLgm, HlLgm2, IrAE, IrAE protein, isae1, isae2, LeG, LEG-1, Leg-2, Leg-4, Leg1, Leg2, Leg3, Leg4, Leg5, legumain, legumain-1, LGMN, lysosomal asparaginycysteine endopeptidase, lysosomal asparaginyl endopeptidase, lysosomal cysteine proteinase, More, N197 legumain, osteoclast inhibitory peptide 2, Ov-aep-1, Phaseolin, Proteinase B, PrVPE1, schistosome legumain, Sj32, Sm32, SmAE, SmAE protein, SPAE, TcLEG3, TcLEG6, TcLEG9, TvAE1, vacuolar processing enzyme, vacuolar processing enzyme 1a, vacuolar processing enzyme 1b, vacuolar processing enzyme 2, vacuolar processing enzyme 3, Vicilin peptidohydrolase, VPE, VPE1a, VPE1b, VPE2, VPE3

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.34 Legumain

Cloned

Cloned on EC 3.4.22.34 - Legumain

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AEP -/-, +/- and +/+ mice on a mixed 129/Ola and C57BL/6 background are generated
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asparaginyl endopeptidase is expressed in Pichia pastoris
C-terminal hexa-His-tagged is expressed in the yeast Pichia pastoris
DNA and amino acid sequence determination
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DNA and amino acid sequence determination and analysis
DNA sequence and structure determination and analysis, expression in mammalian cells
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DNA sequence determination and analysis
DNA sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli BL21, stable expression in 293 cells and secretion to the medium
embryonic kidney cells are stably transfected with human legumain to achieve overexpression in vitro, HEK-Leg cells expressed both active and inactive legumain and secreted it to the medium
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expressed in Escherichia coli
expressed in Escherichia coli, RNA interference applied to silence expression of the SmAE gene encoding schistosome legumain
expressed in HEK-293 cells
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expressed in Pichia pastoris
expressed in Pichia pastoris KM71H cells
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expression as beta-galactosidase fusion protein in Escherichia coli, the recombinant enzyme shows hemoglobinase activity, the N197 enzyme form might not be the active one, but the C197 form
expression in Escherichia coli
expression of C197 in Pichia pastoris, expression as beta-galactosidase fusion protein in Escherichia coli, the recombinant enzyme shows hemoglobinase activity, the N197 enzyme form might not be the active one, but the C197 form
expression of inactive precursor enzyme in yeast and insect cells, expression of a processing-site mutant in yeast and subsequent in vitro processing study
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expression of vegetative enzyme in seeds and of seed enzyme in stamen, phylogenetic tree
gene leg-1, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic tree, expression in Escherichia coli as GST-fusion protein
gene PRSC1, DNA and amino acid sequence determination and analysis, localization on chromosome 14q32.1, expression in insect cells
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gene SPAE or Y192, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression of His-tagged enzyme
glutathione S-transferase-fusion protein expressed in Escherichia coli BL21(DE3)
His-tagged fusion protein stably transfected in LEXSY P10 host strain
in Escherichia coli, Champion-pET directional expression kit, pET100/D-TOPO expression vector, functionally expression of recombinant protein in Pichia pastoris, expression vector pPICZaB
in Escherichia coli, construct pCvsEndoexp1 transformed into callus derived from Q117 meristematic tissue, transgenic plants regenerated
inserted in pET20b(+), transformed into the Escherichia coli BL21(DE3)pLysS strain
overexpression in CHO cells
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overexpression in murine cell line COP5 as a C-terminally IgG-fusion protein
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partial DNA sequence determination
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Pichia pastoris expression system can produce highly active recombinant legumain protein allowing it to be used for high-throughput screening applications
plasmid pTrcHisB/HlLgm transformed into the Escherichia coli Top10F0 strain
precursor of asparaginyl endopeptidase
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recombinant expression in a fully N-glycosylated form
recombinant Ov-AEP-1 is expressed as hexa-His tag in Escherichia coli in inclusion bodies
recombinantly expressed in yeast
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stable overexpression in HEK 293 cells and in C13 cells
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transient expression under control of the CMV promotor of wild-type and mutant enzymes in human embryonic kidney HEK 293 cells, all recombinant enzymes are processed normally
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