3.4.22.34: Legumain
This is an abbreviated version!
For detailed information about Legumain, go to the full flat file.
Word Map on EC 3.4.22.34
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3.4.22.34
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cathepsins
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phaseolus
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vulgaris
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vacuole
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cystatins
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proteinases
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metastasis
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papain
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cotyledon
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plasmodium
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globulin
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falciparum
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vicilins
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schistosoma
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papain-like
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tau
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legume
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endopeptidases
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seed-specific
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mesoamerican
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phytohemagglutinin
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falcipain-2
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clan
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mansoni
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andean
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leguminous
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procathepsins
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lunatus
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cyclotides
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jack
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caspase-1-like
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drug development
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biotechnology
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medicine
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diagnostics
- 3.4.22.34
- cathepsins
- phaseolus
- vulgaris
- vacuole
- cystatins
- proteinases
- metastasis
- papain
- cotyledon
- plasmodium
- globulin
- falciparum
- vicilins
- schistosoma
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papain-like
- tau
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legume
- endopeptidases
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seed-specific
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mesoamerican
- phytohemagglutinin
- falcipain-2
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clan
- mansoni
-
andean
-
leguminous
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procathepsins
- lunatus
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cyclotides
-
jack
-
caspase-1-like
- drug development
- biotechnology
- medicine
- diagnostics
Reaction
hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds =
Synonyms
AEP, Antigen Sj32, Antigen SM32, As-specific vacuolar cysteine proteinase, Asn-specific vacuolar cysteine proteinase, asparagine endopeptidase, asparaginly endopeptidase, asparaginyl endopepidase, Asparaginyl endopeptidase, asparaginyl endopeptidase legumain, asparaginyl endopeptidase,, asparaginyl proteinase, asparaginyl-specific cysteine endopeptidase, asparagyl-endopeptidase, aspartate-specific endopeptidase, AtLEGgamma, Bean endopeptidase, C197 legumain, c197 Sm32, cysteine protease 1, hemoglobinase, HlLgm, HlLgm2, IrAE, IrAE protein, isae1, isae2, LeG, LEG-1, Leg-2, Leg-4, Leg1, Leg2, Leg3, Leg4, Leg5, legumain, legumain-1, LGMN, lysosomal asparaginycysteine endopeptidase, lysosomal asparaginyl endopeptidase, lysosomal cysteine proteinase, More, N197 legumain, osteoclast inhibitory peptide 2, Ov-aep-1, Phaseolin, Proteinase B, PrVPE1, schistosome legumain, Sj32, Sm32, SmAE, SmAE protein, SPAE, TcLEG3, TcLEG6, TcLEG9, TvAE1, vacuolar processing enzyme, vacuolar processing enzyme 1a, vacuolar processing enzyme 1b, vacuolar processing enzyme 2, vacuolar processing enzyme 3, Vicilin peptidohydrolase, VPE, VPE1a, VPE1b, VPE2, VPE3
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3.4.22.34 LegumainAdvanced search results
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results (Natural Substrates Products
Natural Substrates Products on EC 3.4.22.34 - Legumain
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NATURAL SUBSTRATE 
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
acetoacetyl-CoA synthetase + H2O
?
Asn547 is the specific cleavage site of acetoacetyl-CoA synthetase in mouse livers. The cleaved form of acetoacetyl-CoA synthetase (1-547) loses the ability to convert acetoacetate to acetoacetyl-CoA. Cleavage of acetoacetyl-CoA synthetase by legumain is critical for the regulation of enzymatic activity and results in gain-of-function changes. Acetoacetyl-CoA synthetase(1-547) increases the protein expression of caveolin-1, the principal component of the caveolae
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-
?
annexin II + H2O
?
cleavage of the N-terminus, enzyme plays a role in inactivation and degradation of annexin II in endosomes and lysosomes
-
?
bovine albumin + H2O
peptides containing C-terminal Asn
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-
-
?
bovine haemoglobin + H2O
peptides containing C-terminal Asn
exhibited strict specificity for the asparaginyl bonds on the carboxy-terminal side of a peptide
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-
?
papain-like cysteine proteinase precursors + H2O
activated papin-like cysteine proteinases
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involved in the senescing of tissues and cell death
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?
phaseolin + H2O
?
initiation of phaseolin proteolysis, enyme plays a key role in mobilization of phaseolin during and after kidney bean germination
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?
precursor of basic vacuolar chitinase + H2O
active basic vacuolar chitinase
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involved in the senescing of tissues and cell death
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?
progelatinase A + H2O
gelatinase A + ?
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in cell culture of human fibrosarcoma HT1080 cells, 72 kDa proform, activation by cleavage of an asparaginyl peptide bond at Asn109-Tyr110 or Asn111-Phe112 to form the N-terminus of the mature active gelatinase
62 kDa mature form
?
Vicilin + H2O
?
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degradation of the principal reserve protein present in Vigna radiata seeds
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-
?
vitamin D-binding protein + H2O
?
enzyme is involved in the processing of mcromolecules absorbed by proximal tubule cells
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?
Hemoglobin + H2O
?
the enzyme is involved in host hemoglobin degradation
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-
?
Hemoglobin + H2O
?
the enzyme is involved in host hemoglobin degradation
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-
?
protein + H2O
peptides
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involved in mobilization of storage proteins and stress response processes
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?
protein + H2O
peptides
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enzyme is involved in antigen presentation within class II MHC positive cells and in pro-protein processing
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?
protein + H2O
peptides
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enzyme is critically involved in the lysosomal processing of bacterial antigens for MHC class II presentation
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?
protein + H2O
peptides
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enzyme is involved mainly in storage protein breakdown
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?
additional information
?
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during germination and seedling growth legumains might also act as processing enzymes, protein processing and degradation by legumains, overview
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?
additional information
?
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enzyme is a protease which specifically cleaves on the carboxyl side of asparagine residues
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?
additional information
?
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enzyme is a protease which specifically cleaves on the carboxyl side of asparagine residues
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?
additional information
?
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the function of legumain in the endometrium during early placentation may be through its role as an activator of protease zymogens such as progelatinase A
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-
?
additional information
?
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the function of legumain in the endometrium during early placentation may be through its role as an activator of protease zymogens such as progelatinase A
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-
?
additional information
?
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thought to be involved in the hydrolysis of stored seed proteins
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-
?
additional information
?
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during germination and seedling growth legumains might also act as processing enzymes, protein processing and degradation by legumains, overview
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?
additional information
?
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enzyme is involved in host blood-meal digestion
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-
?
additional information
?
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enzyme is involved in host blood-meal digestion
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-
?
additional information
?
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recombinant HlLgm and HlLgm2 efficiently digest blood proteins, haemoglobin and bovine serum albumin
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-
?
additional information
?
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role of legumain in blood feeding and blood-meal digestion in ticks
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-
?
additional information
?
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enzyme inhibits osteoclast-like multinucleated cell formation in humans by more than 60% and bone resorption in the fetal rat long bone
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?
additional information
?
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enzyme inhibits osteoclast-like multinucleated cell formation in humans by more than 60% and bone resorption in the fetal rat long bone
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?
additional information
?
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silencing of endogenous specific inhibitor cystatin M by siRNA leads to increased enzyme activity in MDA-686Ln cells and increasec cell migration/proliferation, and also to reduced cysteine protease inhibitor activity, overview
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?
additional information
?
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the enzyme controls antigen processing performing the key step, mechanism, the enzyme expression is reduced in endotoxin-tolerant monocytes and by lipopolysaccharide priming
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?
additional information
?
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the inhibition of osteoclast formation and bone resorption is a nonenzymatic function of prolegumain
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?
additional information
?
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involved in lysosomal processing of bacterial antigens for MHC class II presentation
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?
additional information
?
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involved in lysosomal processing of bacterial antigens for MHC class II presentation
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?
additional information
?
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enzyme promote cell migration and is associated with enhanced tissue invasion and metastases
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?
additional information
?
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legumain is a member of the C13 family of peptidases that specifically cleaves asparaginyl bonds
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?
additional information
?
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the enzyme is not required for class II MHC antigen presentation but is essential for processing of cathepsin L in mice
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?
additional information
?
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role in extracellular matrix remodeling via degradation of fibronectin indicated
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?
additional information
?
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role in extracellular matrix remodeling via degradation of fibronectin indicated
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?
additional information
?
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asparaginyl endopeptidase plays a pivotal role in the endosome and lysosomal degradation system and is implicated in antigen processing and presentation
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-
?
additional information
?
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asparaginyl endopeptidaseprotein substrates on the C-terminal side of asparagine
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?
additional information
?
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role in backbone cyclization of proteins determined, catalyis of peptide bond cleavage and of ligation of plant-specific cyclotides in a single processing event
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-
?
additional information
?
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during germination and seedling growth legumains might also act as processing enzymes, protein processing and degradation by legumains, overview
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?
additional information
?
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role in backbone cyclization of proteins determined, catalyis of peptide bond cleavage and of ligation of plant-specific cyclotides in a single processing event
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-
?
additional information
?
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asparaginyl endopeptidases are cysteine proteases that hydrolyze peptides and proteins on the carboxyl side of asparagine residues
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-
?
additional information
?
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asparaginyl endopeptidases are cysteine proteases that hydrolyze peptides and proteins on the carboxyl side of asparagine residues
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-
?
additional information
?
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enzyme has high YVADase (caspase-1-like) activity (set to 100%). It also exhibits a significant level of IETDase (caspase-8) activity (39%) and DEVDase activity (18%). Unprocessed VPE1 has residual VEIDase (caspase-6) and LEVDase (caspase-4) activities, and negligible LEHDase (caspase-9) and GRRase (metacaspase) activities
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-
?
additional information
?
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PrVPE1 binds to the DEVD tetrapeptide, a signature substrate for caspase-3
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-
?
additional information
?
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processing enzyme of vacuoles, is responsible for the conversion of several vacuolar precursors into their mature forms
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-
?
additional information
?
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during germination and seedling growth legumains might also act as processing enzymes, protein processing and degradation by legumains, overview
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?
additional information
?
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the enzyme is involved in degradation of seed storage proteins
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-
?
additional information
?
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no pivotal role for cathepsin B1 activation in vivo
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?
additional information
?
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no pivotal role for cathepsin B1 activation in vivo
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-
?
additional information
?
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during germination and seedling growth legumains might also act as processing enzymes, protein processing and degradation by legumains, overview
-
?
additional information
?
-
-
role in backbone cyclization of proteins determined, catalyis of peptide bond cleavage and of ligation of plant-specific cyclotides in a single processing event
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-
?
