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3.4.22.34: Legumain

This is an abbreviated version!
For detailed information about Legumain, go to the full flat file.

Word Map on EC 3.4.22.34

Reaction

hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds =

Synonyms

AEP, Antigen Sj32, Antigen SM32, As-specific vacuolar cysteine proteinase, Asn-specific vacuolar cysteine proteinase, asparagine endopeptidase, asparaginly endopeptidase, asparaginyl endopepidase, Asparaginyl endopeptidase, asparaginyl endopeptidase legumain, asparaginyl endopeptidase,, asparaginyl proteinase, asparaginyl-specific cysteine endopeptidase, asparagyl-endopeptidase, aspartate-specific endopeptidase, AtLEGgamma, Bean endopeptidase, C197 legumain, c197 Sm32, cysteine protease 1, hemoglobinase, HlLgm, HlLgm2, IrAE, IrAE protein, isae1, isae2, LeG, LEG-1, Leg-2, Leg-4, Leg1, Leg2, Leg3, Leg4, Leg5, legumain, legumain-1, LGMN, lysosomal asparaginycysteine endopeptidase, lysosomal asparaginyl endopeptidase, lysosomal cysteine proteinase, More, N197 legumain, osteoclast inhibitory peptide 2, Ov-aep-1, Phaseolin, Proteinase B, PrVPE1, schistosome legumain, Sj32, Sm32, SmAE, SmAE protein, SPAE, TcLEG3, TcLEG6, TcLEG9, TvAE1, vacuolar processing enzyme, vacuolar processing enzyme 1a, vacuolar processing enzyme 1b, vacuolar processing enzyme 2, vacuolar processing enzyme 3, Vicilin peptidohydrolase, VPE, VPE1a, VPE1b, VPE2, VPE3

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.34 Legumain

Purification

Purification on EC 3.4.22.34 - Legumain

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1045fold to homogeneity from kidney
2200fold from kidney, to homogeneity
-
affinity chromatography (glutathione-Sepharose)
ammonium sulfate precipitation
-
from cotyledon
-
native enzyme from dry seeds, to homogeneity
native enzyme from germinated seeds, to homogeneity
native enzyme from kidney, overview
-
native enzyme to homogeneity
recombinant enzyme
-
recombinant enzyme is purified by affinity chromatography using His-Trap nickel columns
recombinant His-tagged enzyme from Escherichia coli
recombinant His-tagged enzyme from Escherichia coli, gel filtration and SDS-PAGE
recombinant His-tagged enzyme from Escherichia coli, SDS-PAGE
recombinant IgG-fusion protein from cell line COP5
-
recombinant protein is purified using Ni-Sepharos under native conditions
recombinnat His-tagged enzyme
SDS-PAGE
soluble recombinant C197 from Pichia pastoris, native enzyme to homogeneity
using Ni–NTA chromatography