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Literature summary for 3.4.22.34 extracted from
- Schistosome asparaginyl endopeptidase (legumain) is not essential for cathepsin B1 activation in vivo (2008), Mol. Biochem. Parasitol., 159, 54-58.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli, RNA interference applied to silence expression of the SmAE gene encoding schistosome legumain | Schistosoma mansoni |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
x * 32000, SDS-PAGE, no signals after RNA interference of the SMAE gene encoding schistosome legumain | Schistosoma mansoni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Schistosoma mansoni | no pivotal role for cathepsin B1 activation in vivo | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schistosoma mansoni | P09841 | product of the SmAE gene | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| SDS-PAGE | Schistosoma mansoni |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| schistosomulum | 6-week-old, male parasites | Schistosoma mansoni | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
expression of the SmAE gene in adult Schistosoma mansoni suppressed by RNA interference, full processing and activity of cathepsin B1 in the absence of detectable SmAE protein observed, experimental data indicate that schistosome legumain is not essential to activate cathepsin B1 in vivo | Schistosoma mansoni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no pivotal role for cathepsin B1 activation in vivo | Schistosoma mansoni | ? | - |
? | |
| additional information | analysis of in vivo processing activity of schistosome legumain to convert cathepsin B1 into its active form, no essential role for cathepsin B1 activation in vivo determined | Schistosoma mansoni | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, SDS-PAGE, no signals after RNA interference of the SMAE gene encoding schistosome legumain | Schistosoma mansoni |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Asparaginyl endopeptidase | - |
Schistosoma mansoni |
| schistosome legumain | - |
Schistosoma mansoni |
| SmAE protein | - |
Schistosoma mansoni |
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Release 2023.1 (January 2023)
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