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Literature summary for 3.4.22.34 extracted from

  • Laha, T.; Sripa, J.; Sripa, B.; Pearson, M.; Tribolet, L.; Kaewkes, S.; Sithithaworn, P.; Brindley, P.J.; Loukas, A.
    Asparaginyl endopeptidase from the carcinogenic liver fluke, Opisthorchis viverrini, and its potential for serodiagnosis (2008), Int. J. Infect. Dis., 12, 49-59.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
diagnostics potential for serodiagnosis of human opisthorchiasisas immunodiagnostic antigen Opisthorchis viverrini

Cloned(Commentary)

Cloned (Comment) Organism
recombinant Ov-AEP-1 is expressed as hexa-His tag in Escherichia coli in inclusion bodies Opisthorchis viverrini

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide complete inhibition at 5 mM inhibitor concentration Opisthorchis viverrini

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
determined by SDS-PAGE Opisthorchis viverrini

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Opisthorchis viverrini asparaginyl endopeptidases are cysteine proteases that hydrolyze peptides and proteins on the carboxyl side of asparagine residues ?
-
?

Organism

Organism UniProt Comment Textmining
Opisthorchis viverrini Q208S4
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein potential N-linked glycosylation sites (NXS/T) are detected at Asn-68 and Asn-173 Opisthorchis viverrini

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme is purified by affinity chromatography using His-Trap nickel columns Opisthorchis viverrini

Renatured (Commentary)

Renatured (Comment) Organism
to obtain bioactive recombinant protein, the protein isolated in its denatured form is refolded by stepwise dialysis (1 M urea increments every 24 h) against phosphate buffered saline, pH 7.4 Opisthorchis viverrini

Source Tissue

Source Tissue Comment Organism Textmining
adult
-
Opisthorchis viverrini
-
egg
-
Opisthorchis viverrini
-
gut
-
Opisthorchis viverrini
-
juvenile
-
Opisthorchis viverrini
-
metacercaria
-
Opisthorchis viverrini
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
the specific activity of recombinant asparaginyl endopeptidase is lower than that detected for excretory-secretory products of adult worm Opisthorchis viverrini

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information asparaginyl endopeptidases are cysteine proteases that hydrolyze peptides and proteins on the carboxyl side of asparagine residues Opisthorchis viverrini ?
-
?
Z-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O
-
Opisthorchis viverrini Z-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
?

Synonyms

Synonyms Comment Organism
Asparaginyl endopeptidase
-
Opisthorchis viverrini
legumain
-
Opisthorchis viverrini
Ov-aep-1
-
Opisthorchis viverrini

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Opisthorchis viverrini

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
in 0.1 M sodium phosphate Opisthorchis viverrini

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
no activity at pH 6.0 Opisthorchis viverrini