Application | Comment | Organism |
---|---|---|
diagnostics | potential for serodiagnosis of human opisthorchiasisas immunodiagnostic antigen | Opisthorchis viverrini |
Cloned (Comment) | Organism |
---|---|
recombinant Ov-AEP-1 is expressed as hexa-His tag in Escherichia coli in inclusion bodies | Opisthorchis viverrini |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | complete inhibition at 5 mM inhibitor concentration | Opisthorchis viverrini |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
determined by SDS-PAGE | Opisthorchis viverrini |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Opisthorchis viverrini | asparaginyl endopeptidases are cysteine proteases that hydrolyze peptides and proteins on the carboxyl side of asparagine residues | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Opisthorchis viverrini | Q208S4 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | potential N-linked glycosylation sites (NXS/T) are detected at Asn-68 and Asn-173 | Opisthorchis viverrini |
Purification (Comment) | Organism |
---|---|
recombinant enzyme is purified by affinity chromatography using His-Trap nickel columns | Opisthorchis viverrini |
Renatured (Comment) | Organism |
---|---|
to obtain bioactive recombinant protein, the protein isolated in its denatured form is refolded by stepwise dialysis (1 M urea increments every 24 h) against phosphate buffered saline, pH 7.4 | Opisthorchis viverrini |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adult | - |
Opisthorchis viverrini | - |
egg | - |
Opisthorchis viverrini | - |
gut | - |
Opisthorchis viverrini | - |
juvenile | - |
Opisthorchis viverrini | - |
metacercaria | - |
Opisthorchis viverrini | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the specific activity of recombinant asparaginyl endopeptidase is lower than that detected for excretory-secretory products of adult worm | Opisthorchis viverrini |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | asparaginyl endopeptidases are cysteine proteases that hydrolyze peptides and proteins on the carboxyl side of asparagine residues | Opisthorchis viverrini | ? | - |
? | |
Z-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O | - |
Opisthorchis viverrini | Z-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Asparaginyl endopeptidase | - |
Opisthorchis viverrini |
legumain | - |
Opisthorchis viverrini |
Ov-aep-1 | - |
Opisthorchis viverrini |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Opisthorchis viverrini |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
in 0.1 M sodium phosphate | Opisthorchis viverrini |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
no activity at pH 6.0 | Opisthorchis viverrini |