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Literature summary for 3.4.22.34 extracted from
- Autocatalytic activation of human legumain at aspartic acid residues (1998), FEBS Lett., 438, 114-118.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis | Mus musculus |
| overexpression in murine cell line COP5 as a C-terminally IgG-fusion protein | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C52A | reduced autocatalytic activity, no activity against other substrates | Homo sapiens |
| H150A | reduced autocatalytic activity, no activity against other substrates | Homo sapiens |
| H47A | reduced autocatalytic activity, no activity against other substrates | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
x * 48000, autocatalytically processed enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein + H2O | Homo sapiens | - |
peptides | - |
? |  |
| protein + H2O | Mus musculus | - |
peptides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | O89017 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | autocatalytic activation by cleavage of aspartic acid residues, overview | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant IgG-fusion protein from cell line COP5 | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O | - |
Homo sapiens | benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| legumain + H2O | autocatalysis | Homo sapiens | processed legumain + N-terminal and C-terminal prodomains | - |
? | |
| additional information | no activity with benzyloxycarbonyl-Gly-Ala-Gln-4-methylcoumarin-7-amide | Homo sapiens | ? | - |
? | |
| protein + H2O | - |
Homo sapiens | peptides | - |
? | |
| protein + H2O | - |
Mus musculus | peptides | - |
? | |
| succinyl-Tyr-Val-Ala-Asn-4-methylcoumarin-7-amide + H2O | - |
Homo sapiens | succinyl-Tyr-Val-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48000, autocatalytically processed enzyme, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.1 | - |
recombinant enzyme, dependent on buffer system | Homo sapiens |
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Release 2023.1 (January 2023)
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