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Literature summary for 3.4.22.34 extracted from
- Does an asparaginyl-specific cysteine endopeptidase trigger phaseolin degradation in cotyledons of kidney bean seedlings ? (1998), Eur. J. Biochem., 258, 546-558.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis | Phaseolus vulgaris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | 11.6% remaining activity | Phaseolus vulgaris |  |
| iodoacetate | 55.8 remaining activity | Phaseolus vulgaris | |
| additional information | no inhibition with EDTA, PMSF, pepstatin A, leupeptin, and E-64 | Phaseolus vulgaris | |
| N-ethylmaleimide | 16.6% remaining activity | Phaseolus vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38100 | - |
x * 38100, mature enzyme, SDS-PAGE, x * 53400, pre-proenzyme, SDS-PAGE | Phaseolus vulgaris |
| 53400 | - |
x * 38100, mature enzyme, SDS-PAGE, x * 53400, pre-proenzyme, SDS-PAGE | Phaseolus vulgaris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phaseolin + H2O | Phaseolus vulgaris | initiation of phaseolin proteolysis, enyme plays a key role in mobilization of phaseolin during and after kidney bean germination | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | O24325 | legumain-like proteinase LLP | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | but enzyme contains 1 putative N-glycosylation site at N326 | Phaseolus vulgaris |
| proteolytic modification | putative cleavage site is N367-E368 | Phaseolus vulgaris |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 44fold | Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | germinated, developmental pattern | Phaseolus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.04 | - |
purified enzyme, substrate casein | Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Phaseolus vulgaris | ? | - |
? | |
| phaseolin + H2O | - |
Phaseolus vulgaris | ? | - |
? | |
| phaseolin + H2O | initiation of phaseolin proteolysis, enyme plays a key role in mobilization of phaseolin during and after kidney bean germination | Phaseolus vulgaris | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38100, mature enzyme, SDS-PAGE, x * 53400, pre-proenzyme, SDS-PAGE | Phaseolus vulgaris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| asparaginyl-specific cysteine endopeptidase | - |
Phaseolus vulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Phaseolus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
assay at | Phaseolus vulgaris |
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Release 2023.1 (January 2023)
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