Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.34 extracted from

  • Sojka, D.; Hajdusek, O.; Dvorak, J.; Sajid, M.; Franta, Z.; Schneider, E.L.; Craik, C.S.; Vancova, M.; Buresova, V.; Bogyo, M.; Sexton, K.B.; McKerrow, J.H.; Caffrey, C.R.; Kopacek, P.
    IrAE: an asparaginyl endopeptidase (legumain) in the gut of the hard tick Ixodes ricinus (2007), Int. J. Parasitol., 37, 713-724.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
in Escherichia coli, Champion-pET directional expression kit, pET100/D-TOPO expression vector, functionally expression of recombinant protein in Pichia pastoris, expression vector pPICZaB Ixodes ricinus

Inhibitors

Inhibitors Comment Organism Structure
N-[(benzyloxy)carbonyl]-L-alanyl-N-[(1S,3E)-1-(2-amino-2-oxoethyl)-5-[methyl(naphthalen-1-yl)amino]-2,5-dioxopent-3-en-1-yl]-L-alaninamide i.e. aza-peptide Michael acceptor Aza-Asn-11a, inhibition of trans-processing and activation of the zymogen form of Schistosoma mansoni cathepsin B1 by prior incubation with the legumain-specific inhibitor Aza-Asn-11a, reaction in CPS buffer, pH 5.5 for 30 min Ixodes ricinus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane association of with microvilli shown Ixodes ricinus 16020
-

Organism

Organism UniProt Comment Textmining
Ixodes ricinus Q6PRC7 adult females
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification C-terminus, in endogenous and in recombinant protein Ixodes ricinus

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli, SDS-PAGE Ixodes ricinus

Source Tissue

Source Tissue Comment Organism Textmining
gut expression in, localized in digestive vesicles of gut cells and within the peritrophic matrix, function associated with the degradation of host hemoglobin suggested Ixodes ricinus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity profile shown, specificity profile using a positional scanning synthetic combinatorial library indicated Ixodes ricinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O activity of recombinant protein determined Ixodes ricinus benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
?
cathepsin B proform + H2O trans-processing and activation of the zymogen form of Schistosoma mansoni cathepsin B1 indicated Ixodes ricinus activated cathepsin B
-
?
Hemoglobin + H2O degradation of human hemoglobin to a major product of 4000 Dalton at pH 4.5, less efficiency at pH 6.0, yielding two products at 6000 and 4000 Dalton, no reaction at pH 7.0 observed, strict specificity for Asn at P1 shown Ixodes ricinus ?
-
?

Synonyms

Synonyms Comment Organism
Asparaginyl endopeptidase
-
Ixodes ricinus
IrAE protein
-
Ixodes ricinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
activity lowered above pH 6.0 Ixodes ricinus

pH Range

pH Minimum pH Maximum Comment Organism
4 6 activity under different pH conditions measured Ixodes ricinus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
IC50 concentration of the aza-peptide Michael acceptor Aza-Asn-11a in a range of about 500 nM determined Ixodes ricinus additional information