Cloned (Comment) | Organism |
---|---|
in Escherichia coli, Champion-pET directional expression kit, pET100/D-TOPO expression vector, functionally expression of recombinant protein in Pichia pastoris, expression vector pPICZaB | Ixodes ricinus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-[(benzyloxy)carbonyl]-L-alanyl-N-[(1S,3E)-1-(2-amino-2-oxoethyl)-5-[methyl(naphthalen-1-yl)amino]-2,5-dioxopent-3-en-1-yl]-L-alaninamide | i.e. aza-peptide Michael acceptor Aza-Asn-11a, inhibition of trans-processing and activation of the zymogen form of Schistosoma mansoni cathepsin B1 by prior incubation with the legumain-specific inhibitor Aza-Asn-11a, reaction in CPS buffer, pH 5.5 for 30 min | Ixodes ricinus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | association of with microvilli shown | Ixodes ricinus | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ixodes ricinus | Q6PRC7 | adult females | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | C-terminus, in endogenous and in recombinant protein | Ixodes ricinus |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli, SDS-PAGE | Ixodes ricinus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gut | expression in, localized in digestive vesicles of gut cells and within the peritrophic matrix, function associated with the degradation of host hemoglobin suggested | Ixodes ricinus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity profile shown, specificity profile using a positional scanning synthetic combinatorial library indicated | Ixodes ricinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O | activity of recombinant protein determined | Ixodes ricinus | benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
cathepsin B proform + H2O | trans-processing and activation of the zymogen form of Schistosoma mansoni cathepsin B1 indicated | Ixodes ricinus | activated cathepsin B | - |
? | |
Hemoglobin + H2O | degradation of human hemoglobin to a major product of 4000 Dalton at pH 4.5, less efficiency at pH 6.0, yielding two products at 6000 and 4000 Dalton, no reaction at pH 7.0 observed, strict specificity for Asn at P1 shown | Ixodes ricinus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Asparaginyl endopeptidase | - |
Ixodes ricinus |
IrAE protein | - |
Ixodes ricinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
activity lowered above pH 6.0 | Ixodes ricinus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | activity under different pH conditions measured | Ixodes ricinus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
IC50 concentration of the aza-peptide Michael acceptor Aza-Asn-11a in a range of about 500 nM determined | Ixodes ricinus | additional information |