3.4.22.34: Legumain
This is an abbreviated version!
For detailed information about Legumain, go to the full flat file.
Word Map on EC 3.4.22.34
-
3.4.22.34
-
cathepsins
-
phaseolus
-
vulgaris
-
vacuole
-
cystatins
-
proteinases
-
metastasis
-
papain
-
cotyledon
-
plasmodium
-
globulin
-
falciparum
-
vicilins
-
schistosoma
-
papain-like
-
tau
-
legume
-
endopeptidases
-
seed-specific
-
mesoamerican
-
phytohemagglutinin
-
falcipain-2
-
clan
-
mansoni
-
andean
-
leguminous
-
procathepsins
-
lunatus
-
cyclotides
-
jack
-
caspase-1-like
-
drug development
-
biotechnology
-
medicine
-
diagnostics
- 3.4.22.34
- cathepsins
- phaseolus
- vulgaris
- vacuole
- cystatins
- proteinases
- metastasis
- papain
- cotyledon
- plasmodium
- globulin
- falciparum
- vicilins
- schistosoma
-
papain-like
- tau
-
legume
- endopeptidases
-
seed-specific
-
mesoamerican
- phytohemagglutinin
- falcipain-2
-
clan
- mansoni
-
andean
-
leguminous
-
procathepsins
- lunatus
-
cyclotides
-
jack
-
caspase-1-like
- drug development
- biotechnology
- medicine
- diagnostics
Reaction
hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds =
Synonyms
AEP, Antigen Sj32, Antigen SM32, As-specific vacuolar cysteine proteinase, Asn-specific vacuolar cysteine proteinase, asparagine endopeptidase, asparaginly endopeptidase, asparaginyl endopepidase, Asparaginyl endopeptidase, asparaginyl endopeptidase legumain, asparaginyl endopeptidase,, asparaginyl proteinase, asparaginyl-specific cysteine endopeptidase, asparagyl-endopeptidase, aspartate-specific endopeptidase, AtLEGgamma, Bean endopeptidase, C197 legumain, c197 Sm32, cysteine protease 1, hemoglobinase, HlLgm, HlLgm2, IrAE, IrAE protein, isae1, isae2, LeG, LEG-1, Leg-2, Leg-4, Leg1, Leg2, Leg3, Leg4, Leg5, legumain, legumain-1, LGMN, lysosomal asparaginycysteine endopeptidase, lysosomal asparaginyl endopeptidase, lysosomal cysteine proteinase, More, N197 legumain, osteoclast inhibitory peptide 2, Ov-aep-1, Phaseolin, Proteinase B, PrVPE1, schistosome legumain, Sj32, Sm32, SmAE, SmAE protein, SPAE, TcLEG3, TcLEG6, TcLEG9, TvAE1, vacuolar processing enzyme, vacuolar processing enzyme 1a, vacuolar processing enzyme 1b, vacuolar processing enzyme 2, vacuolar processing enzyme 3, Vicilin peptidohydrolase, VPE, VPE1a, VPE1b, VPE2, VPE3
ECTree
3.4.22.34 LegumainAdvanced search results
results (
results (Source Tissue
Source Tissue on EC 3.4.22.34 - Legumain
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
SOURCE TISSUE 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
SOURCE
lesions at the aortic sinus and in injured carotid arteries of Apolipoprotein E deficient mice, mRNA expression increases with development of atherosclerosis in the aorta
legumain is overexpressed in the majority of human solid tumours, including breast cancer, colorectal cancer, ovarian cancer, prostate cancer and gastric cancer
for generation of transformed plants, GFP reporter analysis indicated
legumain is overexpressed in the majority of human solid tumours, including breast cancer, colorectal cancer, ovarian cancer, prostate cancer and gastric cancer
human umbilical vascular endothelial cells (HUVECs) tested in migration/proliferation and invasion assays
-
gene TcLeg3 shows an accumulation of transcripts for all analyzed tissues. It is least expressed in the root, which is used as calibrator (1.00X), and is most expressed in the seed (21.7X) and tegument (21.5X). Expression in tegument (21.5X), seed (21.7X), leaf (9.9X), and stalk (17.8X). The TcLeg9 gene shows expression in all organs and tissues analyzed. The leaf is the organ with the highest expression (25.5X) as compared to the calibrator (root). Statistically significant differences are observed in the tegument (4.7X), leaf (25.6X), and stalk (19.0X) as compared to the expression in the root (1.00X)
caecal epithelium, 2-6 week old juveniles and adults, mRNA expression also in metacercariae
-
LGMN expression is increased in hepatocellular carcinoma cells relative to normal hepatocytes in the same specimens
legumain is overexpressed in the majority of human solid tumours, including breast cancer, colorectal cancer, ovarian cancer, prostate cancer and gastric cancer
legumain is overexpressed in the majority of human solid tumours, including breast cancer, colorectal cancer, ovarian cancer, prostate cancer and gastric cancer
-
gene TcLeg3 shows an accumulation of transcripts for all analyzed tissues. It is least expressed in the root, which is used as calibrator (1.00X), and is most expressed in the seed (21.7X) and tegument (21.5X). Expression in tegument (21.5X), seed (21.7X), leaf (9.9X), and stalk (17.8X). The TcLeg9 gene shows expression in all organs and tissues analyzed. The leaf is the organ with the highest expression (25.5X) as compared to the calibrator (root). Statistically significant differences are observed in the tegument (4.7X), leaf (25.6X), and stalk (19.0X) as compared to the expression in the root (1.00X)
control plants and transformed plants, GFP reporter analysis indicated
additional information
legumain is overexpressed in the majority of human solid tumours, including breast cancer, colorectal cancer, ovarian cancer, prostate cancer and gastric cancer
inhibitory effects by phytocystatins against commercial human legumain analyzed
-
developing endosperms of 18 days after flowering, inhibitory effects by cystatins against endogenous legumain activity in barley protein extracts analyzed
anthere only
anthere, ovules, filaments
expression in, localized in digestive vesicles of gut cells and within the peritrophic matrix, function associated with the degradation of host hemoglobin suggested
exclusively expressed in the gut tissue. IsAE1 is the principle legumain transcript in partially engorged females, and demonstrates its strong regulation by on-host feeding in larvae, nymphs and females. IsAE1 is the most highly expressed gut-specific legumain in blood fed ticks where it predominated over all its paralogues
IsAE2 is the predominant gut legumain in unfed nymphs, unfed females and males
protein from kidney tissues used for western blot analysis, unilateral ureteral obstruction in legumain-deficient mice
-
leaf extracts, assay for asparaginyl bond hydrolysis in, analysis of backbone cyclization of endogenous cyclotides in
-
in the leaf tissue the Leg1 is the highest expressed isoform, followed by Leg3
-
gene TcLeg3 shows an accumulation of transcripts for all analyzed tissues. It is least expressed in the root, which is used as calibrator (1.00X), and is most expressed in the seed (21.7X) and tegument (21.5X). Expression in tegument (21.5X), seed (21.7X), leaf (9.9X), and stalk (17.8X). The TcLeg9 gene shows expression in all organs and tissues analyzed. The leaf is the organ with the highest expression (25.5X) as compared to the calibrator (root). Statistically significant differences are observed in the tegument (4.7X), leaf (25.6X), and stalk (19.0X) as compared to the expression in the root (1.00X)
M-CSF differentiated primary macrophages, in vitro expression, also detected in the culture media
of atherosclerotic aorta of aging Apolipoprotein E deficient mice, mRNA expression increases with development of atherosclerosis
RAW264.7 monocyte lysate utilized for monitoring endogenous legumain by designed activity-based probes, lysates from 816 B cells used for detection assay of endogenous legumain
-
in root tissues the isoform Leg1 has the greatest mRNA levels, whereas Leg2 has the lowest ones
-
gene TcLeg3 shows an accumulation of transcripts for all analyzed tissues. It is least expressed in the root, which is used as calibrator (1.00X), and is most expressed in the seed (21.7X) and tegument (21.5X). Expression in tegument (21.5X), seed (21.7X), leaf (9.9X), and stalk (17.8X). The TcLeg9 gene shows expression in all organs and tissues analyzed. The leaf is the organ with the highest expression (25.5X) as compared to the calibrator (root). Statistically significant differences are observed in the tegument (4.7X), leaf (25.6X), and stalk (19.0X) as compared to the expression in the root (1.00X)
-
gene TcLeg3 shows an accumulation of transcripts for all analyzed tissues. It is least expressed in the root, which is used as calibrator (1.00X), and is most expressed in the seed (21.7X) and tegument (21.5X). Expression in tegument (21.5X), seed (21.7X), leaf (9.9X), and stalk (17.8X). The TcLeg9 gene shows expression in all organs and tissues analyzed. The leaf is the organ with the highest expression (25.5X) as compared to the calibrator (root). Statistically significant differences are observed in the tegument (4.7X), leaf (25.6X), and stalk (19.0X) as compared to the expression in the root (1.00X). TcLeg6 is only expressed in the stages of seed development. No transcripts of TcLeg6 gene are detected in the analysis of the mature seed (quiescent) and germinating cotyledon
additional information
-
VPE isoforms are also found during stress response of plant organ, e.g. wounded leaves, the precursor enzyme is synthesized at membrane-bound polysomes in the cytoplasm
additional information
-
the alpha and gamma vacuolar processing enzyme occurs only in vegetative organs
additional information
-
legumain is not detected in salivary glands, ovaries, cuticles, tracheae, Malpighian tubules and synganglia
additional information
tissue distribution, low expression level in all tissues tested, no expression in MG63 osteosarcoma cells
additional information
-
tissue distribution, low expression level in all tissues tested, no expression in MG63 osteosarcoma cells
additional information
-
expression of enzyme in healthy and in cancer cells, the expression is increased in cancer cells, overview
additional information
in all vegetative organs, the precursor enzyme is synthesized at membrane-bound polysomes in the cytoplasm
additional information
tissue-specific and developmental expression patterns, overview
additional information
tissue-specific and developmental expression patterns, overview
additional information
tissue-specific and developmental expression patterns, overview
additional information
tissue-specific and developmental expression patterns, overview
additional information
tissue-specific and developmental expression patterns, overview
additional information
tissue-specific and developmental expression patterns, overview
additional information
tissue-specific and developmental expression patterns, overview
additional information
-
tissue-specific and developmental expression patterns, overview
additional information
-
the precursor enzyme is synthesized at membrane-bound polysomes in the cytoplasm
additional information
-
the precursor enzyme is synthesized at membrane-bound polysomes in the cytoplasm
