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3.4.22.27: cathepsin S

This is an abbreviated version!
For detailed information about cathepsin S, go to the full flat file.

Word Map on EC 3.4.22.27

Reaction

similar to cathepsin L, but with much less activity on Z-Phe-Arg-/-NHMec, and additional information activity on the Z-Val-Val-Arg-/- compound =

Synonyms

C01.034, Cat S, Cat-S, cath S, cathepsin S, cathepsin S-like cysteine proteinase, cathepsin Sa, CatS, CatSPP, CTSS, Hacp-s, PfCTSSa, PoCtS, rs1136774, rs16827671, rs34495036, rs35989725, rs3754212, rs7534124, SoCatS

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.27 cathepsin S

Crystallization

Crystallization on EC 3.4.22.27 - cathepsin S

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2.5 A structure of a complex between human cathepsin S and a potent irreversible inhibitor, vapor diffusion methods at 17°C, where the reservoir contains 1.25 M ammonium sulfate, 70 mM MES, pH 6.5, and 7 mM CoCl2
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cathepsin S mixed with morpholine-4-carboxylic acid [1S-(2-benzyloxy-1R-cyano-ethylcarbamoyl)-3-methylbutyl]amide, crystals are grown by vapor diffusion at room temperature in hanging drops
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cathepsin S-inhibitor complex with the mechanism-based inhibitor Mu(morpholine urea)-Phe-homoPhe-vinylsulfonyl-benzene, vapor diffusion with hanging drops, optimization of parameters results in diffraction-quality crystals produced in 0.2 M (NH4)3PO4, 0.1 M Na3-citrate, pH 5.6 at 17°C. The best crystals to date have been obtained by feeding the crystal drop with 0.0003 ml of protein solution every 5 days for approximately 2 months
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crystal complex of cathepson S with potent covalent inhibitors, microcrystals are grown at 22°C by hanging drop vapor diffusion method
crystallization of the enzyme in complex with an heterocyclic arylaminoethyl amide inhibitor, crystallization in presence of 0.2 M zinc acetate, X-ray diffraction structure determination and analysis at 1.8 A resolution
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determination of the crystal structure of the enzyme cocrystallized with the irreversible vinyl-sulfone inhibitor APC-2848 or with a reversible dipeptide-nitrile inhibitor, crystal structure determination of the C25S mutant cathepsin S
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