Literature summary for 3.4.22.27 extracted from

Br�mme, D.; McGrath, M.E.
High level expression and crystallization of recombinant human cathepsin S (1996), Protein Sci., 5, 789-791.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
cathepsin S-inhibitor complex with the mechanism-based inhibitor Mu(morpholine urea)-Phe-homoPhe-vinylsulfonyl-benzene, vapor diffusion with hanging drops, optimization of parameters results in diffraction-quality crystals produced in 0.2 M (NH4)3PO4, 0.1 M Na3-citrate, pH 5.6 at 17°C. The best crystals to date have been obtained by feeding the crystal drop with 0.0003 ml of protein solution every 5 days for approximately 2 months	Homo sapiens

Crystallization (Comment)

Organism

cathepsin S-inhibitor complex with the mechanism-based inhibitor Mu(morpholine urea)-Phe-homoPhe-vinylsulfonyl-benzene, vapor diffusion with hanging drops, optimization of parameters results in diffraction-quality crystals produced in 0.2 M (NH4)3PO4, 0.1 M Na3-citrate, pH 5.6 at 17°C. The best crystals to date have been obtained by feeding the crystal drop with 0.0003 ml of protein solution every 5 days for approximately 2 months

Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0158	-	benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin	-	Homo sapiens
0.0182	-	benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24000	-	x * 24000, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
testis	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O	-	Homo sapiens	benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin	-	?
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O	-	Homo sapiens	benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin	-	?

Subunits

Subunits	Comment	Organism
?	x * 24000, SDS-PAGE	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.6	-	benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin	-	Homo sapiens
21	-	benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	6.5	-	Homo sapiens