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0.00031 - 0.0435
5'-deoxy-5'-methylthioadenosine
0.00019 - 2.1
5'-methylthioadenosine
0.00055 - 0.055
5'-methylthioinosine
0.004 - 1.3
S-adenosyl-L-homocysteine
0.00000019 - 0.039
S-methyl-5'-thioadenosine
additional information
additional information
-
0.00031
5'-deoxy-5'-methylthioadenosine
-
-
0.00041
5'-deoxy-5'-methylthioadenosine
-
-
0.00725
5'-deoxy-5'-methylthioadenosine
-
-
0.0103
5'-deoxy-5'-methylthioadenosine
-
-
0.015
5'-deoxy-5'-methylthioadenosine
-
-
0.0435
5'-deoxy-5'-methylthioadenosine
-
-
0.00019
5'-methylthioadenosine
-
pH 7.5, 25°C, recombinant enzyme
0.00036
5'-methylthioadenosine
-
pH 7.5, 25°C, recombinant enzyme
0.0004
5'-methylthioadenosine
-
pH 7.5, 25°C, recombinant enzyme
0.0004
5'-methylthioadenosine
-
in 50 mM potassium phosphate buffer, pH 7.0, at 37°C
0.0005
5'-methylthioadenosine
-
-
0.0007
5'-methylthioadenosine
-
Pfs protein, at pH 5.0
0.00124
5'-methylthioadenosine
recombinant enzyme, in 50 mM potassium phosphate, pH 7.0
0.003
5'-methylthioadenosine
-
at 25°C, in 100 mM HEPES, pH 7.5, and 50 mM KCl
0.0034
5'-methylthioadenosine
-
22°C
0.005
5'-methylthioadenosine
-
Bgp protein, at pH 5.0
0.0071
5'-methylthioadenosine
-
22°C
0.0071
5'-methylthioadenosine
-
in 100 mM sodium phosphate buffer, pH 7.0
0.00738
5'-methylthioadenosine
recombinant enzyme, in 50 mM potassium phosphate, pH 7.0
0.023
5'-methylthioadenosine
pH 7.5, 25°C
0.43
5'-methylthioadenosine
-
pH 7.5, 25°C
2.1
5'-methylthioadenosine
pH 7.0, 22°C
0.00055
5'-methylthioinosine
-
-
0.055
5'-methylthioinosine
-
-
0.004
S-adenosyl-L-homocysteine
wild type enzyme, in 100 mM HEPES (pH 7.5), at 25°C
0.0041
S-adenosyl-L-homocysteine
mutant enzyme A15M, in 100 mM HEPES (pH 7.5), at 25°C
0.0056
S-adenosyl-L-homocysteine
mutant enzyme S167A, in 100 mM HEPES (pH 7.5), at 25°C
0.0058
S-adenosyl-L-homocysteine
mutant enzyme V34I, in 100 mM HEPES (pH 7.5), at 25°C
0.0061
S-adenosyl-L-homocysteine
mutant enzyme R85H, in 100 mM HEPES (pH 7.5), at 25°C
0.0062
S-adenosyl-L-homocysteine
mutant enzyme V34A, in 100 mM HEPES (pH 7.5), at 25°C
0.0068
S-adenosyl-L-homocysteine
mutant enzyme R85A, in 100 mM HEPES (pH 7.5), at 25°C
0.0076
S-adenosyl-L-homocysteine
mutant enzyme W179A, in 100 mM HEPES (pH 7.5), at 25°C
0.0098
S-adenosyl-L-homocysteine
mutant enzyme W179F, in 100 mM HEPES (pH 7.5), at 25°C
0.01
S-adenosyl-L-homocysteine
wild type enzyme, at pH 7.2 and 25°C
0.013
S-adenosyl-L-homocysteine
pH 7.5, 25°C
0.0133
S-adenosyl-L-homocysteine
mutant enzyme R164A, in 100 mM HEPES (pH 7.5), at 25°C
0.014
S-adenosyl-L-homocysteine
mutant enzyme H109A, at pH 7.2 and 25°C
0.019
S-adenosyl-L-homocysteine
mutant enzyme F107A, at pH 7.2 and 25°C
0.0381
S-adenosyl-L-homocysteine
mutant enzyme Y134A, in 100 mM HEPES (pH 7.5), at 25°C
0.0452
S-adenosyl-L-homocysteine
mutant enzyme Y134F, in 100 mM HEPES (pH 7.5), at 25°C
0.0951
S-adenosyl-L-homocysteine
mutant enzyme M144A, in 100 mM HEPES (pH 7.5), at 25°C
1.3
S-adenosyl-L-homocysteine
-
pH 7.5, 25°C
0.00000019
S-methyl-5'-thioadenosine
-
in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C
0.00000036
S-methyl-5'-thioadenosine
-
in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C
0.0000004
S-methyl-5'-thioadenosine
-
in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C
0.000061
S-methyl-5'-thioadenosine
wild type enzyme, at pH 7.7 and 25°C
0.000139
S-methyl-5'-thioadenosine
wild type enzyme, at pH 7.7 and 25°C
0.000202
S-methyl-5'-thioadenosine
mutant enzyme A113P/V153I/R158G, at pH 7.7 and 25°C
0.000262
S-methyl-5'-thioadenosine
mutant enzyme A113P, at pH 7.7 and 25°C
0.000262
S-methyl-5'-thioadenosine
mutant enzyme A113P/V153I, at pH 7.7 and 25°C
0.00043
S-methyl-5'-thioadenosine
mutant enzyme V153I, at pH 7.7 and 25°C
0.0009
S-methyl-5'-thioadenosine
wild type enzyme, at pH 7.2 and 25°C
0.0022
S-methyl-5'-thioadenosine
wild type enzyme, in 100 mM HEPES (pH 7.5), at 25°C
0.0029
S-methyl-5'-thioadenosine
mutant enzyme F104C/C186S, at pH 7.2 and 25°C
0.0031
S-methyl-5'-thioadenosine
mutant enzyme A15M, in 100 mM HEPES (pH 7.5), at 25°C
0.0044
S-methyl-5'-thioadenosine
mutant enzyme S167A, in 100 mM HEPES (pH 7.5), at 25°C
0.0045
S-methyl-5'-thioadenosine
mutant enzyme V34I, in 100 mM HEPES (pH 7.5), at 25°C
0.0048
S-methyl-5'-thioadenosine
mutant enzyme R85H, in 100 mM HEPES (pH 7.5), at 25°C
0.0053
S-methyl-5'-thioadenosine
mutant enzyme V34A, in 100 mM HEPES (pH 7.5), at 25°C
0.0054
S-methyl-5'-thioadenosine
mutant enzyme R85A, in 100 mM HEPES (pH 7.5), at 25°C
0.0054
S-methyl-5'-thioadenosine
mutant enzyme W179A, in 100 mM HEPES (pH 7.5), at 25°C
0.0074
S-methyl-5'-thioadenosine
mutant enzyme W179F, in 100 mM HEPES (pH 7.5), at 25°C
0.008
S-methyl-5'-thioadenosine
mutant enzyme R164A, in 100 mM HEPES (pH 7.5), at 25°C
0.019
S-methyl-5'-thioadenosine
-
at pH 7.5 and 25°C
0.028
S-methyl-5'-thioadenosine
mutant enzyme Y134A, in 100 mM HEPES (pH 7.5), at 25°C
0.0301
S-methyl-5'-thioadenosine
mutant enzyme Y134F, in 100 mM HEPES (pH 7.5), at 25°C
0.033
S-methyl-5'-thioadenosine
mutant enzyme H109A, at pH 7.2 and 25°C
0.0351
S-methyl-5'-thioadenosine
mutant enzyme M144A, in 100 mM HEPES (pH 7.5), at 25°C
0.037
S-methyl-5'-thioadenosine
mutant enzyme F107A, at pH 7.2 and 25°C
0.039
S-methyl-5'-thioadenosine
wild type enzyme, at pH 7.2 and 25°C
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
kinetics and thermodynamics, overview
-
additional information
additional information
-
kinetics and thermodynamics, overview
-
additional information
additional information
-
Michelis-Menten kinetics, substrate 5'-deoxyadenosine
-