3.2.2.16: methylthioadenosine nucleosidase

This is an abbreviated version!
For detailed information about methylthioadenosine nucleosidase, go to the full flat file.

Word Map on EC 3.2.2.16

3.2.2.16

mtans

quorum

5'-deoxyadenosine

autoinducer-2

femtomolar

3.2.2.9

5-methylthioribose

adomet-dependent

ribooxacarbenium

s-ribosylhomocysteine

n-ribosidic

futalosine

slow-onset

dadme-immucillins

drug development

medicine

Reaction

Synonyms

5'-deoxy-5'-methylthioadenosine nucleosidase, 5'-methylthioadenosine nucleosidase, 5'-methylthioadenosine/S-adenosyl-L-homocysteine nucleosidase, 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, 5-methylthioadenosine nucleosidase, 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase, 50-methylthioadenosine/S-adenosylhomocysteine nucleosidase, adoHcy/MTA nucleosidase, Bgp protein, glycosaminoglycan-binding protein, HpyMqnB, lupin MeSAdo nucleosidase, MeSAdo nucleosidase, methylthioadenosine nucleosidase, methylthioadenosine/S-adenosylhomocysteine nucleosidase, MqnB, MTA nucleosidase, MTA ribohydrolase, MTA/SAH nucleosidase, MTAN, MTAN1, MTAN2, MTN, Pfs, Pfs protein, plant specific MTA nucleosidase, S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase

ECTree

3 Hydrolases

3.2 Glycosylases

3.2.2 Hydrolysing N-glycosyl compounds

3.2.2.16 methylthioadenosine nucleosidase

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Results	in table
4	Activating Compound
216	AA Sequence
3	Application
1	CAS Registry Number
27	Cloned(Commentary)
20	Crystallization (Commentary)
30	Disease/ Diagnostics
19	General Information
3	General Stability
17	IC50 Value
166	Inhibitors
47	kcat/KM [mM/s]
80	Ki Value [mM]
76	KM Value [mM]
3	Localization
4	Metals/Ions
16	Molecular Weight [Da]
74	Natural Substrates/ Products (Substrates)
289	Organism
6	Pathway
44	PDB ID
14	pH Optimum
3	pH Range
1	pH Stability
40	Protein Variants
34	Purification (Commentary)
13	Reaction
1	Reaction Type
68	Reference
14	Source Tissue
37	Specific Activity [micromol/min/mg]
2	Storage Stability
159	Substrates and Products (Substrate)
16	Subunits
95	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]
2	Temperature Range [°C]
5	Temperature Stability [°C]
57	Turnover Number [1/s]

Purification

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Purification on EC 3.2.2.16 - methylthioadenosine nucleosidase

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6 entries

ammonium sulfate precipitation, acetone precipitation, Sephacryl S-200 gel filtration, DEAE-Sephacel gel filtration, and 5'-AMP-Sepharose column chromatography

Glycine max

728199

ammonium sulfate precipitation, DEAE-Sephadex gel filtration, hydroxyapatite column chromatography, S-formycinylhomocysteine-Sepharose column chromatography, and Sephacryl S-200 gel filtration

Escherichia coli

171854

ammonium sulfate precipitation, nickel affinity column chromatography, and Superdex 200 gel filtration

Brucella melitensis

Q8YBL1

726889

glutathione-Sepharose column chromatography

Arabidopsis thaliana

700813

His-bind resin column chromatography

Burkholderia thailandensis

727555

HisTrap column chromatography and Superdex 200 gel filtration

Helicobacter pylori

Q9ZMY2

727008

HisTrap column chromatography and Superdex 75 gel filtration

homogeneity

native enzyme 675.2fold from cotyledons by ammonium sulfate fractionation, acetone precipitation, gel filtration, anion exchange chromatography, and affinity chromatography

Glycine max

728199

Ni-NTA agarose column chromatography

Arabidopsis thaliana

681477

Ni-NTA agarose column chromatography and Superdex-200 gel filtration

Arabidopsis thaliana

681477

Ni-NTA column chromatography

5 entries

Ni-NTA column chromatography and Superdex 200 gel filtration

Staphylococcus aureus

Q99TQ0

727044

Ni-NTA column chromatography, and gel filtration

Mycobacterium tuberculosis

P9WJN3

731666

Ni-NTA Superflow resin column chromatography and Superdex-200 gel filtration

2 entries

recombinant His-tagged enzyme from Escherichia coli strain BL21trxB by nickel affinity chromatography to over 95% purity

Mycobacterium tuberculosis

P9WJN3

731666

recombinant His-tagged isozyme AtMTAN1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Arabidopsis thaliana

709718

recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration

Helicobacter pylori

O24915

726595

recombinant isozyme AtMTAN2 from Escherichia coli to homogeneity

Arabidopsis thaliana

702045

recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21 Star(DE3) by nickel affinity chromatography

2 entries

recombinant wild-type and mutant His-tagged MTAN from strain BL21(DE3) by nickel affinity chromatography

Escherichia coli

709718