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0.00973 - 20
5'-methylthioadenosine
0.15 - 7.1
S-adenosyl-L-homocysteine
0.0079 - 20
S-methyl-5'-thioadenosine
0.00973
5'-methylthioadenosine
recombinant enzyme, in 50 mM potassium phosphate, pH 7.0
0.25
5'-methylthioadenosine
pH 7.5, 25°C
0.49
5'-methylthioadenosine
recombinant enzyme, in 50 mM potassium phosphate, pH 7.0
2
5'-methylthioadenosine
-
22°C
2
5'-methylthioadenosine
-
at 25°C, in 100 mM HEPES, pH 7.5, and 50 mM KCl
2.3
5'-methylthioadenosine
-
pH 7.5, 25°C, recombinant enzyme
4
5'-methylthioadenosine
-
pH 7.5, 25°C
4.2
5'-methylthioadenosine
-
pH 7.5, 25°C, recombinant enzyme
18.7
5'-methylthioadenosine
-
22°C
18.7
5'-methylthioadenosine
-
in 100 mM sodium phosphate buffer, pH 7.0
20
5'-methylthioadenosine
-
pH 7.5, 25°C, recombinant enzyme
0.15
S-adenosyl-L-homocysteine
mutant enzyme M144A, in 100 mM HEPES (pH 7.5), at 25°C
0.35
S-adenosyl-L-homocysteine
mutant enzyme Y134F, in 100 mM HEPES (pH 7.5), at 25°C
0.37
S-adenosyl-L-homocysteine
pH 7.5, 25°C
0.37
S-adenosyl-L-homocysteine
mutant enzyme W179F, in 100 mM HEPES (pH 7.5), at 25°C
0.39
S-adenosyl-L-homocysteine
mutant enzyme Y134A, in 100 mM HEPES (pH 7.5), at 25°C
0.44
S-adenosyl-L-homocysteine
mutant enzyme F107A, at pH 7.2 and 25°C
0.55
S-adenosyl-L-homocysteine
mutant enzyme S167A, in 100 mM HEPES (pH 7.5), at 25°C
0.74
S-adenosyl-L-homocysteine
mutant enzyme H109A, at pH 7.2 and 25°C
0.86
S-adenosyl-L-homocysteine
mutant enzyme W179A, in 100 mM HEPES (pH 7.5), at 25°C
0.92
S-adenosyl-L-homocysteine
mutant enzyme R85H, in 100 mM HEPES (pH 7.5), at 25°C
0.93
S-adenosyl-L-homocysteine
mutant enzyme R85A, in 100 mM HEPES (pH 7.5), at 25°C
1.1
S-adenosyl-L-homocysteine
mutant enzyme A15M, in 100 mM HEPES (pH 7.5), at 25°C
1.8
S-adenosyl-L-homocysteine
wild type enzyme, at pH 7.2 and 25°C
2.1
S-adenosyl-L-homocysteine
-
pH 7.5, 25°C
2.1
S-adenosyl-L-homocysteine
mutant enzyme V34A, in 100 mM HEPES (pH 7.5), at 25°C
2.3
S-adenosyl-L-homocysteine
mutant enzyme V34I, in 100 mM HEPES (pH 7.5), at 25°C
4.7
S-adenosyl-L-homocysteine
mutant enzyme R164A, in 100 mM HEPES (pH 7.5), at 25°C
7.1
S-adenosyl-L-homocysteine
wild type enzyme, in 100 mM HEPES (pH 7.5), at 25°C
0.0079
S-methyl-5'-thioadenosine
mutant enzyme V153I, at pH 7.7 and 25°C
0.074
S-methyl-5'-thioadenosine
mutant enzyme A113P/V153I, at pH 7.7 and 25°C
0.11
S-methyl-5'-thioadenosine
mutant enzyme A113P/V153I/R158G, at pH 7.7 and 25°C
0.17
S-methyl-5'-thioadenosine
mutant enzyme A113P, at pH 7.7 and 25°C
0.18
S-methyl-5'-thioadenosine
wild type enzyme, at pH 7.7 and 25°C
0.32
S-methyl-5'-thioadenosine
wild type enzyme, at pH 7.7 and 25°C
0.51
S-methyl-5'-thioadenosine
mutant enzyme M144A, in 100 mM HEPES (pH 7.5), at 25°C
0.58
S-methyl-5'-thioadenosine
mutant enzyme S167A, in 100 mM HEPES (pH 7.5), at 25°C
0.58
S-methyl-5'-thioadenosine
mutant enzyme W179F, in 100 mM HEPES (pH 7.5), at 25°C
0.69
S-methyl-5'-thioadenosine
mutant enzyme W179A, in 100 mM HEPES (pH 7.5), at 25°C
0.71
S-methyl-5'-thioadenosine
mutant enzyme Y134F, in 100 mM HEPES (pH 7.5), at 25°C
0.78
S-methyl-5'-thioadenosine
mutant enzyme Y134A, in 100 mM HEPES (pH 7.5), at 25°C
1
S-methyl-5'-thioadenosine
mutant enzyme F107A, at pH 7.2 and 25°C
1.2
S-methyl-5'-thioadenosine
mutant enzyme H109A, at pH 7.2 and 25°C
1.3
S-methyl-5'-thioadenosine
mutant enzyme R85H, in 100 mM HEPES (pH 7.5), at 25°C
1.4
S-methyl-5'-thioadenosine
mutant enzyme R85A, in 100 mM HEPES (pH 7.5), at 25°C
1.8
S-methyl-5'-thioadenosine
-
at pH 7.5 and 25°C
1.8
S-methyl-5'-thioadenosine
mutant enzyme A15M, in 100 mM HEPES (pH 7.5), at 25°C
2.3
S-methyl-5'-thioadenosine
-
in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C
2.5
S-methyl-5'-thioadenosine
mutant enzyme V34I, in 100 mM HEPES (pH 7.5), at 25°C
2.7
S-methyl-5'-thioadenosine
mutant enzyme V34A, in 100 mM HEPES (pH 7.5), at 25°C
3.8
S-methyl-5'-thioadenosine
wild type enzyme, at pH 7.2 and 25°C
4.2
S-methyl-5'-thioadenosine
-
in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C
7.2
S-methyl-5'-thioadenosine
mutant enzyme F104C/C186S, at pH 7.2 and 25°C
8
S-methyl-5'-thioadenosine
mutant enzyme R164A, in 100 mM HEPES (pH 7.5), at 25°C
8.8
S-methyl-5'-thioadenosine
wild type enzyme, in 100 mM HEPES (pH 7.5), at 25°C
10.2
S-methyl-5'-thioadenosine
wild type enzyme, at pH 7.2 and 25°C
20
S-methyl-5'-thioadenosine
-
in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C