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0.5 - 1200
5'-methylthioadenosine
1.6 - 1800
S-adenosyl-L-homocysteine
0.00012 - 50000
S-methyl-5'-thioadenosine
0.5
5'-methylthioadenosine
-
pH 7.5, 25°C, recombinant enzyme
1.2
5'-methylthioadenosine
-
pH 7.5, 25°C, recombinant enzyme
1200
5'-methylthioadenosine
-
pH 7.5, 25°C, recombinant enzyme
1.6
S-adenosyl-L-homocysteine
mutant enzyme M144A, in 100 mM HEPES (pH 7.5), at 25°C
7.8
S-adenosyl-L-homocysteine
mutant enzyme Y134F, in 100 mM HEPES (pH 7.5), at 25°C
10
S-adenosyl-L-homocysteine
mutant enzyme Y134A, in 100 mM HEPES (pH 7.5), at 25°C
23
S-adenosyl-L-homocysteine
mutant enzyme F107A, at pH 7.2 and 25°C
38
S-adenosyl-L-homocysteine
mutant enzyme W179F, in 100 mM HEPES (pH 7.5), at 25°C
51
S-adenosyl-L-homocysteine
mutant enzyme H109A, at pH 7.2 and 25°C
100
S-adenosyl-L-homocysteine
mutant enzyme S167A, in 100 mM HEPES (pH 7.5), at 25°C
110
S-adenosyl-L-homocysteine
mutant enzyme W179A, in 100 mM HEPES (pH 7.5), at 25°C
140
S-adenosyl-L-homocysteine
mutant enzyme R85A, in 100 mM HEPES (pH 7.5), at 25°C
150
S-adenosyl-L-homocysteine
mutant enzyme R85H, in 100 mM HEPES (pH 7.5), at 25°C
170
S-adenosyl-L-homocysteine
wild type enzyme, at pH 7.2 and 25°C
270
S-adenosyl-L-homocysteine
mutant enzyme A15M, in 100 mM HEPES (pH 7.5), at 25°C
330
S-adenosyl-L-homocysteine
mutant enzyme V34A, in 100 mM HEPES (pH 7.5), at 25°C
350
S-adenosyl-L-homocysteine
mutant enzyme R164A, in 100 mM HEPES (pH 7.5), at 25°C
390
S-adenosyl-L-homocysteine
mutant enzyme V34I, in 100 mM HEPES (pH 7.5), at 25°C
1800
S-adenosyl-L-homocysteine
wild type enzyme, in 100 mM HEPES (pH 7.5), at 25°C
0.00012
S-methyl-5'-thioadenosine
-
in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C
0.00012
S-methyl-5'-thioadenosine
-
in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C
15
S-methyl-5'-thioadenosine
mutant enzyme M144A, in 100 mM HEPES (pH 7.5), at 25°C
18
S-methyl-5'-thioadenosine
mutant enzyme V153I, at pH 7.7 and 25°C
24
S-methyl-5'-thioadenosine
mutant enzyme Y134F, in 100 mM HEPES (pH 7.5), at 25°C
27
S-methyl-5'-thioadenosine
mutant enzyme F107A, at pH 7.2 and 25°C
28
S-methyl-5'-thioadenosine
mutant enzyme Y134A, in 100 mM HEPES (pH 7.5), at 25°C
37
S-methyl-5'-thioadenosine
mutant enzyme H109A, at pH 7.2 and 25°C
79
S-methyl-5'-thioadenosine
mutant enzyme W179F, in 100 mM HEPES (pH 7.5), at 25°C
95
S-methyl-5'-thioadenosine
-
at pH 7.5 and 25°C
97
S-methyl-5'-thioadenosine
wild type enzyme, at pH 7.2 and 25°C
130
S-methyl-5'-thioadenosine
mutant enzyme S167A, in 100 mM HEPES (pH 7.5), at 25°C
130
S-methyl-5'-thioadenosine
mutant enzyme W179A, in 100 mM HEPES (pH 7.5), at 25°C
200
S-methyl-5'-thioadenosine
mutant enzyme A113P/V153I/R158G, at pH 7.7 and 25°C
250
S-methyl-5'-thioadenosine
mutant enzyme R85A, in 100 mM HEPES (pH 7.5), at 25°C
280
S-methyl-5'-thioadenosine
mutant enzyme R85H, in 100 mM HEPES (pH 7.5), at 25°C
400
S-methyl-5'-thioadenosine
mutant enzyme A113P/V153I, at pH 7.7 and 25°C
510
S-methyl-5'-thioadenosine
mutant enzyme V34A, in 100 mM HEPES (pH 7.5), at 25°C
560
S-methyl-5'-thioadenosine
mutant enzyme V34I, in 100 mM HEPES (pH 7.5), at 25°C
580
S-methyl-5'-thioadenosine
mutant enzyme A15M, in 100 mM HEPES (pH 7.5), at 25°C
600
S-methyl-5'-thioadenosine
mutant enzyme R164A, in 100 mM HEPES (pH 7.5), at 25°C
700
S-methyl-5'-thioadenosine
mutant enzyme A113P, at pH 7.7 and 25°C
2300
S-methyl-5'-thioadenosine
wild type enzyme, at pH 7.7 and 25°C
2480
S-methyl-5'-thioadenosine
mutant enzyme F104C/C186S, at pH 7.2 and 25°C
3000
S-methyl-5'-thioadenosine
wild type enzyme, at pH 7.7 and 25°C
4000
S-methyl-5'-thioadenosine
wild type enzyme, in 100 mM HEPES (pH 7.5), at 25°C
11300
S-methyl-5'-thioadenosine
wild type enzyme, at pH 7.2 and 25°C
50000
S-methyl-5'-thioadenosine
-
in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C