Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.2.16: methylthioadenosine nucleosidase

This is an abbreviated version!
For detailed information about methylthioadenosine nucleosidase, go to the full flat file.

Word Map on EC 3.2.2.16

Reaction

S-methyl-5'-thioadenosine
+
H2O
=
S-methyl-5-thio-D-ribose
 +
adenine

Synonyms

5'-deoxy-5'-methylthioadenosine nucleosidase, 5'-methylthioadenosine nucleosidase, 5'-methylthioadenosine/S-adenosyl-L-homocysteine nucleosidase, 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, 5-methylthioadenosine nucleosidase, 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase, 50-methylthioadenosine/S-adenosylhomocysteine nucleosidase, adoHcy/MTA nucleosidase, Bgp protein, glycosaminoglycan-binding protein, HpyMqnB, lupin MeSAdo nucleosidase, MeSAdo nucleosidase, methylthioadenosine nucleosidase, methylthioadenosine/S-adenosylhomocysteine nucleosidase, MqnB, MTA nucleosidase, MTA ribohydrolase, MTA/SAH nucleosidase, MTAN, MTAN1, MTAN2, MTN, Pfs, Pfs protein, plant specific MTA nucleosidase, S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.16 methylthioadenosine nucleosidase

Cloned

Cloned on EC 3.2.2.16 - methylthioadenosine nucleosidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as His-tagged protein
-
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21 (DE3) Rosetta cells
expressed in Escherichia coli BL21 cells and in Nicotiana benthamiana
-
expressed in Escherichia coli BL21 Codon+ cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21trxB cells
expression in Escherichia coli
gene inactivation/replacement with Escherichia coli pfs gene, cloning of the yrrU gene, PCR amplified
-
gene mnqB, expression of recombinant His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3)
gene RV0091, recombinant expression of active His-tagged enzyme in Escherichia coli strain BL21trxB, optimization of expression method
isozyme AtMTAN1, overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
isozymes AtMTAN1 and AtMTAN2, DNA and amino acid sequence determination and analysis, overexpression of isozyme AtMTAN2 in Escherichia coli
-
overexpression of wild-type and mutant His-tagged enzyme in strain BL21(DE3)
-
recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21 Star(DE3)