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Literature summary for 3.2.2.16 extracted from
- Studies on phosphate-activated 5'-methylthioadenosine nucleosidase from human placenta (1978), Int. J. Biochem., 9, 35-41.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | requires reducing agents, only 67% of maximal activity is observable in absence | Homo sapiens |  |
| dithiothreitol | requires reducing agents, only 67% of maximal activity is observable in absence, optimal concentration 2 mM | Homo sapiens | |
| phosphate | - |
Homo sapiens | |
General Stability
| General Stability | Organism |
|---|---|
| moderately stable | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoic acid | mM PCMB causes quantitative inhibition | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0435 | - |
5'-deoxy-5'-methylthioadenosine | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
3 * 31000, postulated | Homo sapiens |
| 95000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-methylthioadenosine + H2O | Homo sapiens | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
human | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| placenta | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.75 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-deoxy-5'-S-adenosyl-L-cysteine + H2O | relative activity 95.2% | Homo sapiens | S-(5-deoxy-5-D-ribosyl)-L-cysteine + adenine | - |
? | |
| 5'-deoxy-5'-S-adenosyl-L-homocysteine + H2O | substrate analogue, relative activity 95.8% | Homo sapiens | S-(5-deoxy-5-D-ribosyl)-L-homoscysteine + adenine | - |
? | |
| 5'-deoxy-5'-thioethanoladenosine + H2O | relative activity 98% | Homo sapiens | ? | - |
? | |
| 5'-methylthioadenosine + H2O | - |
Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 31000, postulated | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 67 | - |
- |
Homo sapiens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | quite stable in this range | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | quite stable in this range | Homo sapiens |
| 67 | - |
enzyme activity rapidly dropping at temperatures above | Homo sapiens |
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