Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-Chloroformycin | powerful inhibitor | Escherichia coli | |
5'-isobutylthio-3-deaza-adenosine | poor inhibitor | Escherichia coli | |
5'-Isobutylthioinosine | - |
Escherichia coli | |
5'-methylthio-3-deaza-adenosine | poor inhibitor | Escherichia coli | |
5'-Methylthioformycin | powerful inhibitor | Escherichia coli | |
5'-methylthiotubercidin | powerful inhibitor | Escherichia coli | |
5'-n-butylthioinosine | - |
Escherichia coli | |
adenine | poor inhibitor | Escherichia coli | |
additional information | not inhibited by 5'-methylthioinosine, S-inosyl-L-homocysteine, S-guanosyl-L-homocysteine, S-N6-methyl-3-deaza-adenosyl-L-homocysteine, S-adenosyl-L-homocysteine dialdehyde, acyclic adenosyl-L-homocysteine, S-cytidylhomocysteine, hypoxanthine, guanine, and AMP | Escherichia coli | |
S-8-aza-adenosyl-L-homocysteine | powerful inhibitor | Escherichia coli | |
S-adenosyl-L-homocysteine sulfoxide | poor inhibitor | Escherichia coli | |
S-formycinylhomocysteine | powerful inhibitor | Escherichia coli | |
S-N6-dimethyl-3-deaza-adenosyl-L-homocysteine | poor inhibitor | Escherichia coli | |
S-Tubercidinylhomocysteine | powerful inhibitor | Escherichia coli | |
sinefungin | poor inhibitor | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
5'-methylthioadenosine | in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sephadex gel filtration, hydroxyapatite column chromatography, S-formycinylhomocysteine-Sepharose column chromatography, and Sephacryl S-200 gel filtration | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.038 | - |
crude extract, with 5'-methylthioadenosine as substrate, in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli |
373 | - |
after 9810fold purification, with 5'-methylthioadenosine as substrate, in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 50 mM potassium phosphate buffer (pH 7.0), at least 2 months, no significant loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-isobutylthioadenosine + H2O | 52% activity compared to 5'-methylthioadenosine | Escherichia coli | ? | - |
? | |
5'-methylthioadenosine + H2O | 100% activity | Escherichia coli | ? | - |
? | |
5'-n-butylthioadenosine + H2O | 40% activity compared to 5'-methylthioadenosine | Escherichia coli | ? | - |
? | |
additional information | no activity toward 5'-methylthioinosine, 5'-isobutylthioinosine, 5'-isobutylthio-3-deaza-adenosine, 5'-methylthio-3-deaza-adenosine, 5'-chloroformycin, 5'-methylthioformycin, 5'-ethylthiotubercidin, S-tubercidinylhomocysteine, S-inosyl-L-homocysteine, S-guanosyl-L-homocysteine, S-cytidylhomocysteine, S-8-aza adenosyl-L-homocysteine, S-n-methyl-3-deaza-adenosyl-L-homocysteine, S-n6-dimethyl-3-deaza-adenosyl-L-homocysteine, sinefungin, S-adenosyl-L-homocysteine dialdehyde, and acyclic adenosyl-L-homocysteine | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adoHcy/MTA nucleosidase | - |
Escherichia coli |
S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the enzyme is rapidly inactivated after exposure for 15 min at 60°C | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000097 | - |
S-formycinylhomocysteine | in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | |
0.000027 | - |
5'-Methylthioformycin | in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | |
0.000322 | - |
5'-Chloroformycin | in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | |
0.000928 | - |
S-8-aza-adenosyl-L-homocysteine | in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | |
0.0019 | - |
S-Tubercidinylhomocysteine | in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | |
0.0041 | - |
5'-methylthiotubercidin | in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00002 | - |
in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | S-formycinylhomocysteine | |
0.00006 | - |
in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | 5'-Methylthioformycin | |
0.0004 | - |
in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | 5'-Chloroformycin | |
0.0018 | - |
in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | S-8-aza-adenosyl-L-homocysteine | |
0.0032 | - |
in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | S-Tubercidinylhomocysteine | |
0.0077 | - |
in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | 5'-methylthiotubercidin | |
0.013 | - |
in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | 5'-isobutylthio-3-deaza-adenosine | |
0.02 | - |
in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | S-N6-dimethyl-3-deaza-adenosyl-L-homocysteine | |
0.024 | - |
in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | sinefungin | |
0.069 | - |
in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | S-adenosyl-L-homocysteine sulfoxide | |
0.079 | - |
in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | 5'-methylthio-3-deaza-adenosine | |
0.094 | - |
in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | 5'-n-butylthioinosine | |
0.132 | - |
in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | 5'-Isobutylthioinosine | |
0.216 | - |
in 50 mM potassium phosphate buffer, pH 7.0, at 37°C | Escherichia coli | adenine |