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Literature summary for 3.2.2.16 extracted from

  • Guranowski, A.B.; Chiang, P.K.; Cantoni, G.L.
    5'-Methylthioadenosine nucleosidase. Purification and characterization of the enzyme from Lupinus luteus seeds (1981), Eur. J. Biochem., 114, 293-299.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
3-Deazaadenine
-
Lupinus luteus
9-erythro-(2-hydroxyl 3-nonyl)adenine
-
Lupinus luteus
adenine potent competitive inhibitor, feedback inhibition by one of its products Lupinus luteus
Adenosine 5'-carboxamide
-
Lupinus luteus
Decoyinine
-
Lupinus luteus
sinefungin
-
Lupinus luteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00031
-
5'-deoxy-5'-methylthioadenosine
-
Escherichia coli
0.00041
-
5'-deoxy-5'-methylthioadenosine
-
Lupinus luteus
0.055
-
5'-methylthioinosine
-
Lupinus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
-
Escherichia coli
31000
-
2 * 31000, SDS-PAGE Lupinus luteus
62000
-
gel filtration Lupinus luteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-methylthioadenosine + H2O Escherichia coli
-
?
-
?
5'-methylthioadenosine + H2O Lupinus luteus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Lupinus luteus
-
yellow lupin
-

Purification (Commentary)

Purification (Comment) Organism
-
Lupinus luteus

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Lupinus luteus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27
-
-
Lupinus luteus

Storage Stability

Storage Stability Organism
-20°C, 50 mM Bicine buffer, 5% glycerol buffer, stable for at least 3 months, tolerates repeated freezing and thawing Lupinus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-deoxy-5'-isobutylthio-adenosine + H2O 1-butanol buffer/propan-2-ol buffer Lupinus luteus 5-isobutylthio-D-ribose + adenine
-
?
5'-deoxy-5'-propylthioadenosine + H2O 1-butanol buffer/propan-2-ol buffer Lupinus luteus 5-propylthio-D-ribose + adenine
-
?
5'-deoxyadenosine + H2O 1-butanol buffer Lupinus luteus 5-deoxy-D-ribose + adenine
-
?
5'-ethylthio-2-aminoadenosine + H2O 1-butanol buffer/propan-2-ol buffer Lupinus luteus 5-ethylthio-D-ribose + 2-aminoadenosine
-
?
5'-ethylthioadenosine + H2O 1-butanol buffer Lupinus luteus 5-ethylthio-D-ribose + adenine
-
?
5'-ethylthioinosine + H2O 1-butanol buffer Lupinus luteus 5-ethylthio-D-ribose + hypoxanthine
-
?
5'-isobutylthio-3-deaza-adenosine + H2O 1-butanol buffer/propan-2-ol buffer Lupinus luteus 5-isobutylthio-D-ribose + 3-deazadenosine
-
?
5'-isobutylthioinosine + H2O 1-butanol buffer Lupinus luteus 5-isobutylthio-D-ribose + hypoxanthine
-
?
5'-methylthioadenosine + H2O
-
Escherichia coli ?
-
?
5'-methylthioadenosine + H2O
-
Lupinus luteus ?
-
?
5'-methylthioarabinosyladenine + H2O 1-butanol buffer/propan-2-ol buffer Lupinus luteus 5-methylthioarabinose + adenine
-
?
5'-methylthioinosine + H2O maximal velocity about 50-times lower than for 5'-methylthioadenosine Lupinus luteus 5-methylthio-D-ribose + hypoxanthine
-
?
5'-n-butylthioadenosine + H2O 1-butanol buffer Lupinus luteus 5-n-butylthio-D-ribose + adenine
-
?
5'-n-butylthioinosine + H2O 1-butanol buffer Lupinus luteus 5-n-butylthio-D-ribose + hypoxanthine
-
?
S-adenosylmercaptoethanol + H2O 1-butanol buffer/propan-2-ol buffer Lupinus luteus ?
-
?
S-inosylmercaptoethanol + H2O propan-2-ol buffer Lupinus luteus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 31000, SDS-PAGE Lupinus luteus

Synonyms

Synonyms Comment Organism
lupin MeSAdo nucleosidase
-
Lupinus luteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
50 mM potassium phosphate buffer Escherichia coli
8 8.5
-
Lupinus luteus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5
-
Lupinus luteus