Any feedback?
Literature summary for 3.2.2.16 extracted from
- Molecular determinants of substrate specificity in plant 5-methylthioadenosine nucleosidases (2008), J. Mol. Biol., 378, 112-128.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 Codon+ cells | Arabidopsis thaliana |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| apo-enzyme as well as in complex with substrate-and transition-state-analogues 5'-methylthiotubercidin and formycin A, at 2.0-1.8 A resolution. Comparison with isoform MTAN2 | Arabidopsis thaliana |
| homology model of structure based on isoform MTAN1 | Arabidopsis thaliana |
| in complex with 5'-methylthiotubercidin or formycin A, hanging drop vapour diffusion method, in 70 mM sodium acetate trihydrate, pH 4.6, 5.6% (w/v) PEG 4000, 30% (v/v) glycerol | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | residues L181/M168 and F148/L135 in isoforms MTAN1 and MTAN2, respectively, are responsible for the divergence in specificity of the isoforms | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-methylthiotubercidin | transition state analogue | Arabidopsis thaliana |  |
| Formycin A | transition state analogue | Arabidopsis thaliana | |
| Tris | Tris buffer severely inhibits | Arabidopsis thaliana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0034 | - |
5'-methylthioadenosine | 22°C | Arabidopsis thaliana | |
| 0.0071 | - |
5'-methylthioadenosine | 22°C | Arabidopsis thaliana | |
| 0.0071 | - |
5'-methylthioadenosine | in 100 mM sodium phosphate buffer, pH 7.0 | Arabidopsis thaliana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Arabidopsis thaliana | - |
isoform MTAN1 | - |
| Arabidopsis thaliana | - |
isoform MTAN2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography | Arabidopsis thaliana |
| Ni-NTA agarose column chromatography and Superdex-200 gel filtration | Arabidopsis thaliana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.38 | - |
substrate adenosine, 22°C | Arabidopsis thaliana |
| 0.46 | - |
substrate 5'-chloroadenosine, 22°C | Arabidopsis thaliana |
| 0.83 | - |
substrate 5'-chloroadenosine, 22°C | Arabidopsis thaliana |
| 0.9 | - |
substrate S-adenosylhomocysteine, 22°C | Arabidopsis thaliana |
| 1.06 | - |
substrate 5'-iodoadenosine, 22°C | Arabidopsis thaliana |
| 1.36 | - |
substrate 5'-phenylthioadenosine, 22°C | Arabidopsis thaliana |
| 1.5 | - |
substrate 5'-butylthioadenosine, 22°C | Arabidopsis thaliana |
| 1.85 | - |
substrate 5'-isobutylthioadenosine, 22°C | Arabidopsis thaliana |
| 1.98 | - |
substrate 5'-isobutylthioadenosine, 22°C | Arabidopsis thaliana |
| 2.44 | - |
substrate 5'-phenylthioadenosine, 22°C | Arabidopsis thaliana |
| 2.6 | - |
substrate 5'-ethylthioadenosine, 22°C | Arabidopsis thaliana |
| 2.72 | - |
substrate 5'-butylthioadenosine, 22°C | Arabidopsis thaliana |
| 3.14 | - |
substrate 5'-iodoadenosine, 22°C | Arabidopsis thaliana |
| 4.42 | - |
substrate 5'-ethylthioadenosine, 22°C | Arabidopsis thaliana |
| 6.25 | - |
substrate 5'-methylthioadenosine, 22°C | Arabidopsis thaliana |
| 11.05 | - |
substrate 5'-methylthioadenosine, 22°C | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-butylthioadenosine + H2O | - |
Arabidopsis thaliana | 5-butylthio-D-ribose + adenine | - |
? | |
| 5'-butylthioadenosine + H2O | 25% activity compared to 5'-methylthioadenosine | Arabidopsis thaliana | adenine + 5-butylthio-D-ribose | - |
? | |
| 5'-chloroadenosine + H2O | - |
Arabidopsis thaliana | 5-chloro-D-ribose + adenine | - |
? | |
| 5'-chloroadenosine + H2O | 4% activity compared to 5'-methylthioadenosine | Arabidopsis thaliana | adenine + 5-chloro-D-ribose | - |
? | |
| 5'-ethylthioadenosine + H2O | - |
Arabidopsis thaliana | 5-ethylthio-D-ribose + adenine | - |
? | |
| 5'-ethylthioadenosine + H2O | 40% activity compared to 5'-methylthioadenosine | Arabidopsis thaliana | adenine + 5-ethylthio-D-ribose | - |
? | |
| 5'-iodoadenosine + H2O | 28% activity compared to 5'-methylthioadenosine | Arabidopsis thaliana | adenine + 5-iodo-D-ribose | - |
? | |
| 5'-iodothioadenosine + H2O | - |
Arabidopsis thaliana | 5-iodothio-D-ribose + adenine | - |
? | |
| 5'-isobutylthioadenosine + H2O | - |
Arabidopsis thaliana | 5-isobutylthioribose + adenine | - |
? | |
| 5'-isobutylthioadenosine + H2O | 17% activity compared to 5'-methylthioadenosine | Arabidopsis thaliana | adenine + 5-isobutylthio-D-ribose | - |
? | |
| 5'-methylthioadenosine + H2O | - |
Arabidopsis thaliana | 5-methylthio-D-ribose + adenine | - |
? | |
| 5'-methylthioadenosine + H2O | 100% activity | Arabidopsis thaliana | 5-methylthio-D-ribose + adenine | - |
? | |
| 5'-phenylthioadenosine + H2O | - |
Arabidopsis thaliana | 5-phenylthio-D-ribose + adenine | - |
? | |
| 5'-phenylthioadenosine + H2O | 12% activity compared to 5'-methylthioadenosine | Arabidopsis thaliana | adenine + 5-phenylthio-D-ribose | - |
? | |
| adenosine + H2O | - |
Arabidopsis thaliana | adenine + D-ribose | - |
? | |
| additional information | no substrate: S-adenosylhomocysteine, adenosine | Arabidopsis thaliana | ? | - |
? | |
| additional information | no activity towards S-adenosyl-L-homocysteine and adenosine | Arabidopsis thaliana | ? | - |
? | |
| S-adenosyl-L-homocysteine + H2O | 14% activity compared to 5'-methylthioadenosine | Arabidopsis thaliana | adenine + S-ribosyl-L-homocysteine | - |
? | |
| S-adenosylhomocysteine + H2O | - |
Arabidopsis thaliana | adenine + S-ribosylhomocysteine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | x-ray crystallography | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5'-methylthioadenosine nucleosidase | - |
Arabidopsis thaliana |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Arabidopsis thaliana |
| MTA nucleosidase | - |
Arabidopsis thaliana |
| MTA/SAH nucleosidase | - |
Arabidopsis thaliana |
| MTAN1 | - |
Arabidopsis thaliana |
| MTAN2 | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
5'-methylthioadenosine | 22°C | Arabidopsis thaliana | |
| 18.7 | - |
5'-methylthioadenosine | 22°C | Arabidopsis thaliana | |
| 18.7 | - |
5'-methylthioadenosine | in 100 mM sodium phosphate buffer, pH 7.0 | Arabidopsis thaliana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Arabidopsis thaliana |
| 8 | - |
- |
Arabidopsis thaliana |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
