Any feedback?
Literature summary for 3.2.2.16 extracted from
- 5'-Methylthioadenosine nucleosidase, some characteristics of an enzyme from Acetabularia (1979), Dev. Cell Biol., 3, 241-254.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | enzyme activity stabilized by | Acetabularia acetabulum |  |
General Stability
| General Stability | Organism |
|---|---|
| enzyme activity stabilized by glutathione | Acetabularia acetabulum |
| enzyme activity stabilized by L-cysteine | Acetabularia acetabulum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenine | 70% inhibition | Acetabularia acetabulum | |
| adenosine | 85% inhibition | Acetabularia acetabulum | |
| adenosine | inhibits 30% of the total activity of the enzyme | Escherichia coli | |
| Adenosine 5'-carboxamide | - |
Acetabularia acetabulum | |
| CuSO4 | - |
Acetabularia acetabulum | |
| EDTA | - |
Acetabularia acetabulum | |
| F- | 5-10 mM | Acetabularia acetabulum | |
| S-adenosylhomocysteine | competitive inhibition | Acetabularia acetabulum | |
| S-adenosylmethionine | competitive inhibition | Acetabularia acetabulum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00725 | - |
5'-deoxy-5'-methylthioadenosine | - |
Acetabularia acetabulum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Acetabularia acetabulum | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | enzyme activity depended upon, optimum concentration 50-120 mM | Acetabularia acetabulum | |
| Mn2+ | enzyme activity depended upon, optimum concentration 50-120 mM | Acetabularia acetabulum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
gel filtration | Acetabularia acetabulum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-methylthioadenosine + H2O | Escherichia coli | - |
? | - |
? | |
| 5'-methylthioadenosine + H2O | Acetabularia acetabulum | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetabularia acetabulum | - |
unicellular uninuclear green alga | - |
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-methylthioadenosine + H2O | - |
Escherichia coli | ? | - |
? | |
| 5'-methylthioadenosine + H2O | - |
Acetabularia acetabulum | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Acetabularia acetabulum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
at temperatures above, the activity decreases, heating at 100°C for 3 min results in complete inhibition | Acetabularia acetabulum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
- |
Acetabularia acetabulum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | Tris-HCl buffer | Acetabularia acetabulum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
