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3.2.1.26: beta-fructofuranosidase

This is an abbreviated version!
For detailed information about beta-fructofuranosidase, go to the full flat file.

Word Map on EC 3.2.1.26

Reaction

raffinose
+
H2O
=
beta-D-fructofuranose
 +
6-O alpha-D-galactopyranosyl-alpha-D-glucopyranose

Synonyms

A/N-InvG, acid invertase, Acid sucrose-6-phosphate hydrolase, acINV, AFR529Wp, AI, AIV, alkaline invertase, alkaline invertase InvA, alkaline invertase InvB, alkaline/neutral invertase, Atbetafruct4, b-fructofuranosidase, beta-(1-2)-fructofuranosidase, beta-D-fructofuranosidase, beta-D-fructofuranosidase fructohydrolase, beta-D-fructofuranoside fructohydrolase, beta-FFase, beta-fructofuranosidase, beta-fructofuranosidase I, beta-fructofuranoside fructohydrolyase, beta-fructosidase, beta-h-fructosidase, BfrA, BlsacA, Bmsuc1, cell wall invertase, cell wall invertase 4, cell wall-bound invertase, cell wall-bound ivertase, cell-wall invertase, cell-wall invertase 1, CIN, Cin1, CIN12, Cin5, CINV1, CscA, CWI, CWIN, CWIN1, Cwinv-1, cwINV1, cytosolic invertase, EINV1, EINV2, EINV3, EINV4, FCWI, Ffase, FFase I, Ffh, fructofuranosidase, beta-, fructosylinvertase, glucosucrase, INAC-INV, INCW2, INV, Inv-CW, INV-E, Inv-V, INV1p, INV2, INVA, INVB, invertase, invertase 1, invertase 2, Invertase E1, invertase SUC2, invertin, lbbetafruct2, lbbetafruct3, Lin5, Lin6, Lin7, Lyc e 2.01, Lyc e 2.02, maxinvert L 1000, More, neutral invertase, NI, Nin88, OsCIN1, OsCIN2, OsCIN3, Protein B46, re-INVB, SacA, saccharase, SAI, soluble acid invertase, Suc2, SucB, sucrase, Sucrase E1, Sucrose-6-phosphate hydrolase, TIV-1, Uninv, uninv2, Vacuolar invertase, vacuolar invertase 1, vacuolar invertase CvINV, vacuolar invertase NvINV, VIN, VIN1, VIN2, Vinv-1, VINV1, VINV2, VINV3, yeast invertase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.26 beta-fructofuranosidase

Cloned

Cloned on EC 3.2.1.26 - beta-fructofuranosidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
48 h in Nicotiana benthamiana leaves infiltrated by Agrobacterium tumefaciens C58C1 with entry vector pDONR(TM)Zeo and destination vector pMDC32
-
CIN1, functional expression of the enzyme in roots of Arabidopsis thaliana under control of the root-specific pyk10 promoter
CIN12 is inserted into the expression vector pET_ZZ_1a, transformed into Escherichia coli BL21 DE3 and overexpressed
-
cloned into a pET29a-derived vector with an N-terminal His6 tag. Likewise, the truncated InvB covering residues Thr9-Ser457 is constructed for crystallization. Both the wild-type and mutant proteins are overexpressed in Escherichia coli strain BL21(DE3). Selenomethionine (SeMet)-substituted full-length and truncated InvB are overexpressed in Escherichia coli B834 (DE3)
DNA and amino acid sequence determination, expression of His-tagged wild-type and selenomethionine-labeled enzyme in Escherichia coli strain BL21(DE3)
DNA and amino acid sequence determination, map-based cloning of soluble isozyme
-
Escherichia coli BL21 (DE3) with invbpetK plasmid, 37°C, 2TY medium
-
expressed in Arabidopsis thaliana
-
expressed in Escherichia coli and in an invertase-negative strain of Saccharomyces cerevisiae
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli DH5alpha cells
expressed in Escherichia coli Rosetta 2 cells
expressed in Escherichia coli Top10 cells
expressed in Escherichia coli XL1-Blue cells
expressed in methylotrophic yeast Pichia pastoris
expressed in Nicotiana tabacum and Medicago truncatula roots
-
expressed in Pichia pastoris
expressed in Saccharomyces cerevisiae and Pichia pastoris
expressed in Solanum tuberosum cultivar Desiree
-
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli BL21 (DE3), cloning in Escherichia coli DH5alpha, plasmid pET30 (Ek/LIC), 37 degrees Celsius, LB and 2TY medium
-
expression in Hansenula polymorpha and Pichia pastoris
-
expression in Pichia pastoris
expression in Pichia pastoris under the control of the strong AOX1 promoter. The expression levels of INVA(AOX1) (1660 U/mg) and INVB(AOX1) (1993 U/mg) in Pichia pastoris are 9- and 7-fold higher than the expression levels observed for the native INVA and INVB proteins in Zymomonas mobilis. Glycosylation of INVA(AOX1) and INVB(AOX1) plays an important role in their thermal stability, catalytic efficiency, and tolerance to sucrose
-
expression in Pichia pastoris X-33 and Escherichia coli
expression in Saccharomyces cerevisiae
expression in Schizosaccharomyces pombe
-
expression of a LIN6 promoter-beta glucuronidase reporter gene fusion in tobacco
expression of cell wall-bound isozyme Cin1 in transgenic tobacco plants leads to delayed senescence with the recombinant enzyme substituting cytokinin in function
-
expression of His-tagged wild-type enzyme and mutants E190A and E190D in Escherichia coli strain BL21(DE3)
expression of the enzyme in Vicia narbonensis maturing embryos
-
gene cscA, genetic organization, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain XL1-Blue and as soluble, His-tagged enzyme in strain BL21(DE3)
gene Ivr2, expression analysis in response to waterstress, abscisic acid, glucose and polyethylene glycol in leaves and roots, overview
gene lbbetafruct2, DNA and amino acid sequence determination and analysis, expression of the His-tagged soluble, vacuolar isozyme in Pichia pastoris
gene lbbetafruct3, DNA and amino acid sequence determination and analysis, expression of the His-tagged soluble, vacuolar isozyme in Pichia pastoris
genes from the Cu-tolerant population (EhCvINV) and non-tolerant population (EhNvINV) are heterologously expressed in Pichia pastoris
heterologous overexpression in both Escherichia coli and Leishmania tarentolae
isolation and characterization of an invertase and its repressor genes
-
isozyme betafruct1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, very poor expression of His-tagged isozyme betafruct1 in Pichia pastoris strain YIT1
isozyme betafruct2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged isozyme betafruct2 in Pichia pastoris strain YIT2
isozyme betafruct3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged isozyme betafruct3 in Pichia pastoris strain YIT3
isozymes SAI and CWI, DNA and amino acid sequence determinations and analysis
-
Lin8 400 bp fragment inserted into plant transformation vector pK7GWIWG2(II), transformed into tomato plants via Agrobacterium tumefaciens-mediated gene transfer
-
mutant gene amplification with phage P1-derived artificial chromosome clone P0451A10 for sequencing
-
mutants are expressed in Pichia pastoris
-
overexpression as truncated cytosolic protein in Escherichia coli
-
overexpression of a truncated version of InvA (residues Lys9-Thr460)
Pichia pastoris with vector pPICZalphaC
Pichia pastoris X-33, His-6-tagged recombinant wild-type and mutant cDNA in plasmid pBolT3 in expression vector pPICZalphaB, 30°C, buffered glycerol-complex medium, pH 6.0
-
S. cervisiae SEY 2101 transformed with pVT103-L plasmid containing invertase fragment
sequence analysis