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3.2.1.26: beta-fructofuranosidase

This is an abbreviated version!
For detailed information about beta-fructofuranosidase, go to the full flat file.

Word Map on EC 3.2.1.26

Reaction

raffinose
+
H2O
=
beta-D-fructofuranose
+
6-O alpha-D-galactopyranosyl-alpha-D-glucopyranose

Synonyms

A/N-InvG, acid invertase, Acid sucrose-6-phosphate hydrolase, acINV, AFR529Wp, AI, AIV, alkaline invertase, alkaline invertase InvA, alkaline invertase InvB, alkaline/neutral invertase, Atbetafruct4, b-fructofuranosidase, beta-(1-2)-fructofuranosidase, beta-D-fructofuranosidase, beta-D-fructofuranosidase fructohydrolase, beta-D-fructofuranoside fructohydrolase, beta-FFase, beta-fructofuranosidase, beta-fructofuranosidase I, beta-fructofuranoside fructohydrolyase, beta-fructosidase, beta-h-fructosidase, BfrA, BlsacA, Bmsuc1, cell wall invertase, cell wall invertase 4, cell wall-bound invertase, cell wall-bound ivertase, cell-wall invertase, cell-wall invertase 1, CIN, Cin1, CIN12, Cin5, CINV1, CscA, CWI, CWIN, CWIN1, Cwinv-1, cwINV1, cytosolic invertase, EINV1, EINV2, EINV3, EINV4, FCWI, Ffase, FFase I, Ffh, fructofuranosidase, beta-, fructosylinvertase, glucosucrase, INAC-INV, INCW2, INV, Inv-CW, INV-E, Inv-V, INV1p, INV2, INVA, INVB, invertase, invertase 1, invertase 2, Invertase E1, invertase SUC2, invertin, lbbetafruct2, lbbetafruct3, Lin5, Lin6, Lin7, Lyc e 2.01, Lyc e 2.02, maxinvert L 1000, More, neutral invertase, NI, Nin88, OsCIN1, OsCIN2, OsCIN3, Protein B46, re-INVB, SacA, saccharase, SAI, soluble acid invertase, Suc2, SucB, sucrase, Sucrase E1, Sucrose-6-phosphate hydrolase, TIV-1, Uninv, uninv2, Vacuolar invertase, vacuolar invertase 1, vacuolar invertase CvINV, vacuolar invertase NvINV, VIN, VIN1, VIN2, Vinv-1, VINV1, VINV2, VINV3, yeast invertase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.26 beta-fructofuranosidase

Activating Compound

Activating Compound on EC 3.2.1.26 - beta-fructofuranosidase

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
14-3-3 protein
combined interaction with 14-3-3 protein and calcium-dependent kinase 3 enhances enzyme activity
-
2-mercaptoethanol
-
134.4% activity at 1 mM
abscisic acid
water stress and abscisic acid activate the vacuolar isozyme and rapidly induce the expression of gene Ivr2 encoding the vacuolar isozyme in leaves and roots, in addition to accumulation of hexoses, glucose supply or polyethylene glycol at 1% do not directly activate the isozyme but induce its expression
Alkaline phosphatase
-
336 mM, 27% inhibition
-
Arabidopsis thaliana cell wall/vacuolar inhibitor of fructosidase 2
-
122% activation of vacuolar invertase
-
Beta vulgaris cell wall/vacuolar inhibitor of fructosidase
-
135% activation of vacuolar inveraase
-
beta-Lactoglobulin
-
336 mM, 27% inhibition
-
bovine serum albumin
-
carboxymethylcellulose
-
33.17% increase of activity at 0.5 mM
-
cis-(+)-abscisic acid
-
-
concanavalin A
-
activates isozyme IT II best at 0.9 mg/ml
-
Cys
-
1-10 mM, activates
D-glucose
-
stimulates activity 2.68fold at 40 mM
D-sorbitol cinnamic ester
-
intense hydrophobic interactions between cinnamoyl groups of the compound with related groups of the enzyme immobilizes the enzyme and enhances substrate affinity and hydrolysis rate, immobilization at pH 5.5, 4°C, 12 h
deoxycholic acid
-
10 mM, 137% of initial activity activity
Dextran sulfate
-
-
-
dithiothreitol
-
1 mM, 134% of initial activity
DTNB
-
0.5 mM, 11% activation of isozyme IT II, 24% inhibition of isozyme IT I
heparin
-
activates
Human gamma-globulin
-
336 mM, 27% inhibition
-
iodoacetamide
-
enhances activity
K2HPO4
-
0.05% (w/v) KH2PO4 stimulates the production of FFase to 18%
lectin
-
lectins specific for alpha-mannose or alpha-glucose, activate. Lectins specific for N-acetylglucosamine oligomers, N-acetylgalactosamine or galactose do not activate
-
MgSO4
-
119.76% increase of activity at 0.5 mM
NaBr
-
130.9% activity at 1 mM
NaCl
-
120% activity at 1 mM
NH4F
-
130.5% activity at 1 mM
Nicotiana tabacum vacuolar inhibitor of fructosidase
-
154% activation of vacuolar invertase
-
ovalbumin
-
activates
-
Polyvinylpyrrolidone
reduced glutathione
-
activates
Triton X-100
-
10 mM, 138% of initial activity activity
Tween-80
-
10 mM, 138% of initial activity activity
urease
-
activates isozyme IT II best at 0.1 mg/ml
-
Zn(NO3)2
-
155.4% activity at 1 mM
additional information
-