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3.2.1.26: beta-fructofuranosidase

This is an abbreviated version!
For detailed information about beta-fructofuranosidase, go to the full flat file.

Word Map on EC 3.2.1.26

Reaction

raffinose
+
H2O
=
beta-D-fructofuranose
 +
6-O alpha-D-galactopyranosyl-alpha-D-glucopyranose

Synonyms

A/N-InvG, acid invertase, Acid sucrose-6-phosphate hydrolase, acINV, AFR529Wp, AI, AIV, alkaline invertase, alkaline invertase InvA, alkaline invertase InvB, alkaline/neutral invertase, Atbetafruct4, b-fructofuranosidase, beta-(1-2)-fructofuranosidase, beta-D-fructofuranosidase, beta-D-fructofuranosidase fructohydrolase, beta-D-fructofuranoside fructohydrolase, beta-FFase, beta-fructofuranosidase, beta-fructofuranosidase I, beta-fructofuranoside fructohydrolyase, beta-fructosidase, beta-h-fructosidase, BfrA, BlsacA, Bmsuc1, cell wall invertase, cell wall invertase 4, cell wall-bound invertase, cell wall-bound ivertase, cell-wall invertase, cell-wall invertase 1, CIN, Cin1, CIN12, Cin5, CINV1, CscA, CWI, CWIN, CWIN1, Cwinv-1, cwINV1, cytosolic invertase, EINV1, EINV2, EINV3, EINV4, FCWI, Ffase, FFase I, Ffh, fructofuranosidase, beta-, fructosylinvertase, glucosucrase, INAC-INV, INCW2, INV, Inv-CW, INV-E, Inv-V, INV1p, INV2, INVA, INVB, invertase, invertase 1, invertase 2, Invertase E1, invertase SUC2, invertin, lbbetafruct2, lbbetafruct3, Lin5, Lin6, Lin7, Lyc e 2.01, Lyc e 2.02, maxinvert L 1000, More, neutral invertase, NI, Nin88, OsCIN1, OsCIN2, OsCIN3, Protein B46, re-INVB, SacA, saccharase, SAI, soluble acid invertase, Suc2, SucB, sucrase, Sucrase E1, Sucrose-6-phosphate hydrolase, TIV-1, Uninv, uninv2, Vacuolar invertase, vacuolar invertase 1, vacuolar invertase CvINV, vacuolar invertase NvINV, VIN, VIN1, VIN2, Vinv-1, VINV1, VINV2, VINV3, yeast invertase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.26 beta-fructofuranosidase

Temperature Range

Temperature Range on EC 3.2.1.26 - beta-fructofuranosidase

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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 70
15 - 35
15°C: about % of maximal activity, 356°C: about % of maximal activity
17 - 50
-
INVA: same optimal temperature at 30°C, and similar course above that, retaining about 60% activity of the immobilized enzyme at 40°C, about 40% of the free enzyme, below 20% activity retained at 50°C
20 - 45
-
20°C: about 80% of maximal activity, 45°C: about 70% of maximal activity
20 - 50
20°C: about 60% of maximal activity, 50°C: about 45% of maximal activity
20 - 55
-
20°C: about 25% of maximal activity, 55°C: about 45% of maximal activity
20 - 60
-
immobilized enzyme: about 50% of maximal activity at 20°C, about 40% of maximal activity at 60°C, free enzyme: about 65% of maximal activity at 20°C, about 40% of maximal activity at 50°C, almost no activity at 60°C
20 - 70
-
20°C: about 50% of maximal activity, 70°C: about 55% of maximal activity, enzyme expressed in Pichia pastoris
25 - 45
-
25°C: about 45% of maximal activity, 45°C: about 45% of maximal activity, fructofuranosidase activity
25 - 50
-
chimeric fusion enzyme INVA-CBD, immobilized enzyme with higher activities above optimal temperature of 30°C, close to 60% activity at 40°C retained, while in free enzyme only about 20%, about 40% retained at 45-50°C in the immobilized enzyme while only about 20% in the free enzyme, at 25°C immobilized enzyme with about 40% activity, free enzyme with about 70%
25 - 55
-
25°C: about 45% of maximal activity, 50°C: about 90% of maximal activity, 55°C: about 25% of maximal activity, invertase activity
25 - 60
25 - 90
less than 10% activity at 25 and above 80°C, 100% activity at 65-70°C, about 90% activity at 60 and 75°C, about 50% activity at 50-55°C, 10-40% activity at 35-45°C
30 - 60
30 - 80
-
30°C: about 80% of maximal activity of F-form of invertase, about 75% of maximal activity of S-form of invertase, 80°C: about 55% of maximal activity of F-form of invertase, 98% of maximal activity of S-form of invertase
30 - 90
-
30°C: about 75% of maximal activity of soluble S-form, about 40% of maximal activity of immobilized S-form, about 60% of maximal activity of immobilized F-form, 90°C: about 20% of maximal activity of immobilized S-form, about 15% of maximal activity of soluble S-form, about 50% of maximal activity of immobilized F-form, about 30% of maximal activity of soluble F-form
35 - 55
more than 50% activity between 35 and 55°C
40 - 70
40 - 75
40 - 80
40°C: about 60% of maximal activity, 80°C: about 10% of maximal activity
40 - 90
-
40°C: about 35% of maximal activity, 90°C: about 30% of maximal activity
45 - 60
-
45°C: about 80% of maximal activity, 60°C: about 30% of maximal activity
5 - 30
-
active in the range
50 - 80
60 - 98
-
60°C: about 35% of maximal activity, 98°C: about 20% of maximal activity
additional information