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2.2
fructosylnystose
-
pH 5.7, 37°C
1.7
neokestose
-
pH 5.7, 37°C
additional information
additional information
-
1.3
1-kestose
-
in 0.2M sodium acetate buffer (pH 5.6), at 50°C
1.7
1-kestose
-
pH 5.7, 37°C
4.5
1-kestose
pH 5.5, 42°C, alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranosyl-(1,2)-beta-D-fructofuranose
49
1-kestose
-
pH 5.0, 37°C
700
1-kestose
-
in 50 mM sodium acetate buffer (pH 5.0), at 30°C
22.5
cellobiose
-
in 100 mM sodium acetate buffer, pH 5.0, at 50°C
123.6
cellobiose
-
in the presence of Mn2+, in 100 mM sodium acetate buffer, pH 5.0, at 50°C
0.9
inulin
37°C, pH 7.5
2.66
inulin
-
in sodium acetate buffer, 100 mM, pH 4.5, at 60°C
7
inulin
-
mutant N254A, pH 5.0
9
inulin
-
mutant Y462A, pH 5.0
12
inulin
-
wild-type, pH 5.0
14
inulin
-
mutant Q435A, pH 5.0
15.6
inulin
-
pH 5.0, 37°C
24
inulin
-
mutant Q176E, pH 5.0
25.9
inulin
-
pH 5.7, 37°C
44
inulin
-
mutant S281I, pH 5.0
47
inulin
-
mutant Q228V, pH 5.0
60
inulin
-
mutant N142Y, pH 5.0
575
inulin
-
mutant Q176S, pH 5.0
1.6
nystose
-
in 0.2M sodium acetate buffer (pH 5.6), at 50°C
3.8
nystose
-
mutant Q435A, pH 5.0
3.9
nystose
-
pH 5.7, 37°C
8
nystose
-
mutant N142Y, pH 5.0
8
nystose
-
mutant N254A, pH 5.0
8
nystose
-
mutant Y462A, pH 5.0
8.1
nystose
-
mutant Q176E, pH 5.0
9
nystose
-
mutant Q228V, pH 5.0
11
nystose
-
mutant Q176S, pH 5.0
15
nystose
-
pH 5.0, 37°C
15
nystose
-
mutant S281I, pH 5.0
1.3
raffinose
-
in 0.2M sodium acetate buffer (pH 5.6), at 50°C
1.6
raffinose
-
invertase I
2.9
raffinose
-
invertase I
4.5
raffinose
-
pH 5.0, 40°C
7.37
raffinose
-
in sodium acetate buffer, 100 mM, pH 4.5, at 60°C
7.6
raffinose
-
enzyme form F-1
10.6
raffinose
-
beta-FFase L, pH 5.1
10.8
raffinose
-
invertase II
13
raffinose
-
invertase III
16.4
raffinose
-
55°C, pH 4.6, invertase 2
16.7
raffinose
-
enzyme form F-2
17
raffinose
-
invertase II
19.6
raffinose
-
soluble enzyme
20.4
raffinose
-
beta-FFase E, pH 5.1
20.7
raffinose
-
pH 7.5, alkaline invertase
21.7
raffinose
-
beta-FFase L, pH 3.6
32.19
raffinose
pH 5.0, 37°C
55.98
raffinose
-
40°C, pH 4.5
125
raffinose
-
beta-FFase E, pH 3.6
141
raffinose
37°C, pH 7.5
150
raffinose
-
extracellular and intracellular enzyme
150
raffinose
-
exteracellular enzyme
392
raffinose
-
pH 5.0, 37°C
14
stachyose
-
invertase I
25
stachyose
-
invertase II
168
stachyose
37°C, pH 7.5
434.42
stachyose
-
40°C, pH 4.5
0.00829
sucrose
-
37°C, pH not specified in the publication
0.01582
sucrose
-
37°C, pH not specified in the publication
0.06528
sucrose
-
37°C, pH not specified in the publication
0.104
sucrose
-
pH and temperature not specified in the publication
0.1193
sucrose
-
37°C, pH not specified in the publication
0.282
sucrose
30°C, pH 5.0
0.32
sucrose
-
neutral invertase
0.37
sucrose
-
pH and temperature not specified in the publication
0.56
sucrose
-
saline-released acid invertase
0.65
sucrose
-
native enzyme
0.65
sucrose
-
invertase 1
0.98
sucrose
-
invertase 2
1
sucrose
-
invertase IIA
1.08
sucrose
-
at pH 5.5 and 55°C
1.37
sucrose
pH 7.5, 37°C, wild-type enzyme
1.4
sucrose
-
enzyme embedded within a polyacrylamide gel
1.43
sucrose
-
deglycosylated enzyme
1.54
sucrose
-
pH 4.7, 30°C, F-form, extracellular
1.7
sucrose
-
invertase IIB
1.7
sucrose
-
cell-wall bound enzyme
1.8
sucrose
-
acid invertase, pH 6.5, 37°C
2
sucrose
-
pH 4.7, 30°C, S-form, extracellular
2
sucrose
in 50 mM acetate buffer (pH 5.0) at 37°C
2
sucrose
-
pH and temperature not specified in the publication
2
sucrose
pH and temperature not specified in the publication
2.1
sucrose
-
invertase P-2
2.17
sucrose
-
beta-FFasse-E, pH 5.1
2.4
sucrose
-
invertase I
2.4
sucrose
-
pH 7.0, 37°C
2.4
sucrose
-
pH 6.8, 37°C
2.4
sucrose
-
wild-type, pH 5.0
2.46
sucrose
pH 7.0, 50°C
2.5
sucrose
-
extracellular enzyme, pH 4.5, 60°C
2.68
sucrose
30°C, pH 5.0
2.77
sucrose
30°C, pH 5.0
2.8
sucrose
-
acid invertase
2.94
sucrose
-
pH and temperature not specified in the publication
3 - 3.5
sucrose
-
native invertase, pre-incubation at 80°C for 20 min, 55°C, pH 4.5
3.3
sucrose
-
pH and temperature not specified in the publication
3.33
sucrose
-
pH 4.5, 30°C, isoenzyme AIV I
3.45
sucrose
-
beta-FFase E, pH 4.5
3.5
sucrose
-
soluble enzyme
3.5
sucrose
-
pH 5.0, 40°C
3.57
sucrose
-
in MES buffer, pH 5.0, at 40°C
3.7
sucrose
-
beta-FFase L, pH 5.1
3.7
sucrose
-
pH 5.0, 37°C, isozyme IT II
3.8 - 4.4
sucrose
-
acid invertases
4
sucrose
pH 5.5, 42°C, alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranose
4
sucrose
-
pH and temperature not specified in the publication
4.4
sucrose
-
invertase II
4.4
sucrose
-
invertase P-1
4.41
sucrose
-
pH 6.5, 37°C, hyperbolic saturation kinetics
4.58
sucrose
-
pH 4.5, 30°C, isoenzyme AIV II
4.76
sucrose
-
beta-FFase L, pH 4.5
4.8
sucrose
-
pH and temperature not specified in the publication
4.89
sucrose
-
pH and temperature not specified in the publication
4.9
sucrose
-
in 0.2M sodium acetate buffer (pH 5.6), at 50°C
4.97
sucrose
pH 5.0, recombinant isozyme lbbetafruct3
5
sucrose
-
enzyme forms INV1 and INV3
5
sucrose
-
mutant Q176S, pH 5.0
5.8
sucrose
pH 5.6, 28°C, wild-type
5.89
sucrose
-
beta-FFase L, pH 3.6
6.2
sucrose
-
enzyme form F-1
6.2
sucrose
-
55°C, pH 4.6, invertase 2
6.4
sucrose
-
invertase II
6.4
sucrose
-
wild-type, pH 5.0
6.6
sucrose
-
invertase III
6.6
sucrose
-
pH 6.0, 37°C
6.7
sucrose
-
invertase II
6.9
sucrose
-
enzyme adsorbed on CM-cellulose
7
sucrose
-
mutant Q176E, pH 5.0
7
sucrose
-
mutant Y462A, pH 5.0
7.2
sucrose
-
invertase entrapped into calcium alginate beads
7.43
sucrose
-
immobilized enzyme, pH 6.0, 45°C
7.69
sucrose
-
beta-FFase E, pH 3.6
8
sucrose
-
mutant N254A, pH 5.0
8.1
sucrose
-
invertase I
8.8
sucrose
pH 5.6, 28°C, W440Y
8.9
sucrose
pH 7.7, 30°C, invertase InvB, wild-type enzyme
9
sucrose
-
mutant Q435A, pH 5.0
9.1
sucrose
-
free enzyme, pH 5.0, 30°C
9.8
sucrose
-
55°C, pH 4.6, invertase 1
9.85
sucrose
pH 7.5, 37°C, mutant enzyme E234A
10
sucrose
-
pH and temperature not specified in the publication
10.05
sucrose
-
25% xylitol, 55°C, pH 4.5
10.1
sucrose
pH 5.0, recombinant isozyme lbbetafruct2
10.69
sucrose
pH 5.0, 37°C
10.75
sucrose
-
30% glycerol, 55°C, pH 4.5
10.9
sucrose
-
pH 7.0, 37°C, isozyme IT I
11
sucrose
-
40°C, pH 5.5
11
sucrose
-
40°C, pH 5.5
11
sucrose
-
40°C, pH 5.5
11
sucrose
-
mutant Q228V, pH 5.0
11
sucrose
-
pH and temperature not specified in the publication
11
sucrose
-
pH and temperature not specified in the publication
11.5
sucrose
-
15% sorbitol, 55°C, pH 4.5
11.9
sucrose
-
enzyme form F-2
11.9
sucrose
-
in the presence of Mn2+, in 100 mM sodium acetate buffer, pH 5.0, at 50°C
12
sucrose
-
enzyme form INV2
12.3
sucrose
-
extracellular invertase I
12.5
sucrose
-
native invertase, 55°C, pH 4.5
13
sucrose
-
mutant S281I, pH 5.0
13.14
sucrose
pH 7.5, 37°C, mutant enzyme D63A
13.4
sucrose
-
in sodium acetate buffer, 100 mM, pH 4.5, at 60°C
13.4
sucrose
-
pH and temperature not specified in the publication
14
sucrose
-
85°C, pH 6.0
14
sucrose
-
pH and temperature not specified in the publication
14
sucrose
at pH 4.5 and 50°C
14.3
sucrose
-
pH 6.8, neutral invertase
15.1
sucrose
-
pH 8.0, alkaline invertase
16.66
sucrose
-
40% xylitol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5
17.8
sucrose
at pH 4.5 and 45°C
17.85
sucrose
-
40% glycerol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5
18.1
sucrose
pH 7.7, 30°C, invertase InvB, mutant enzyme R430N
18.6
sucrose
-
pH and temperature not specified in the publication
18.7
sucrose
-
pH 7.5, neutral invertase
19
sucrose
-
mutant N24S, pH 4.8, 50°C
19.23
sucrose
-
40% glycerol, 55°C, pH 9
20
sucrose
-
neutral and alkaline invertase
20.8
sucrose
pH 7.9, 30°C, invertase InvA, wild-type enzyme
21
sucrose
pH and temperature not specified in the publication
21.27
sucrose
-
40% sorbitol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5
21.73
sucrose
-
40% xylitol, 55°C, pH 9
22.7
sucrose
-
in 100 mM sodium acetate buffer, pH 5.0, at 50°C
24.5
sucrose
-
free enzyme, pH 4.5, 57°C
25
sucrose
-
extracellular enzyme
25
sucrose
-
pH and temperature not specified in the publication
25 - 26
sucrose
-
extracellular enzyme
25.55
sucrose
-
40°C, pH 4.5
25.6
sucrose
-
isoforms EINV1, EIV2, EINV3, EINV4, pH 4.5, 25°C
26
sucrose
-
extracellular enzyme
27.1
sucrose
in 100 mM Na-acetate, pH 4.6, and 100 mM sucrose, at 35°C
27.1
sucrose
pH 7.9, 30°C, invertase InvA, mutant enzyme R429N
29
sucrose
-
mutant N142Y, pH 5.0
29.41
sucrose
-
native invertase, 55°C, pH 9
30.7
sucrose
pH and temperature not specified in the publication
35.67
sucrose
-
pH and temperature not specified in the publication
37.6
sucrose
-
immobilized enzyme, pH 4.5, 57°C
38
sucrose
-
pH 5.7, 37°C
38
sucrose
-
wild-type, pH 4.8, 50°C
38.6
sucrose
-
alkaline invertase, pH 6.5, 37°C
41
sucrose
-
pH 5.0, 35°C, invertase INVA expressed in Pichia pastoris under the control of the strong AOX1 promoter
41.2
sucrose
-
free enzyme
42
sucrose
-
pH 7.0, 30°C
46.5
sucrose
-
enzyme immobilized on polyurethane rigid adhesive foam, pH 5.0, 50°C
50
sucrose
-
pH 5.5, 40°C, deglycosylated invertase INVB
50
sucrose
-
pH 5.5, 40°C, invertase INVB expressed in Pichia pastoris under the control of the strong AOX1 promoter
51
sucrose
-
pH 5.5, 30°C, free chimeric fusion enzyme INVA-CBD
53.68
sucrose
-
pH 5.0, 37°C, wild-type enzyme
57
sucrose
Corynebacterium murisepticum
-
-
60
sucrose
-
pH and temperature not specified in the publication
61.2
sucrose
-
free enzyme, pH 5.0, 50°C
70.4
sucrose
-
covalently bound enzyme, pH 5.0, 30°C
78
sucrose
-
immobilized enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5
78.6
sucrose
pH 5.0, 60°C
83
sucrose
-
pH 5.0, 35°C, deglycosylated invertase INVA
86
sucrose
-
pH and temperature not specified in the publication
89.3
sucrose
pH 7.9, 30°C, invertase InvA, mutant enzyme R429A
93
sucrose
-
40°C, pH 5.0, without ultrasound
94
sucrose
-
40°C, pH 5.0, with ultrasound
98
sucrose
-
free enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5.5
98
sucrose
-
pH 5.5, 40°C, native invertase INVB
110
sucrose
-
pH 5.5, 30°C, free INVA
110
sucrose
-
pH 5.0, 35°C, native invertase INVA
130
sucrose
-
adsorbed enzyme, pH 5.0, 30°C
140.7
sucrose
-
pH 5.0, 37°C, mutant enzyme C317A
142.6
sucrose
-
pH 5.0, 37°C, mutant enzyme E316Q
153
sucrose
-
pH 5.5, 30°C, cellulose immobilized chimeric fusion enzyme INVA-CBD
160
sucrose
-
pH 5.0, 37°C
160.6
sucrose
-
pH 5.0, 50°C, 55 mg/ml
169.8
sucrose
-
pH 5.0, 37°C, mutant enzyme W159F
189
sucrose
-
pH 5.5, 30°C, Nylon-6 immobilized INVA
213.6
sucrose
-
pH 5.0, 37°C, mutant enzyme W159L
227
sucrose
-
pH 4.5, 35°C
227
sucrose
-
pH and temperature not specified in the publication
230
sucrose
-
in 50 mM sodium acetate buffer (pH 5.0), at 30°C
230
sucrose
-
pH and temperature not specified in the publication
290
sucrose
-
pH and temperature not specified in the publication
370
sucrose
-
pH 6.0, 37°C
370
sucrose
-
pH and temperature not specified in the publication
398
sucrose
-
pH 5.0, 37°C
470
sucrose
-
pH and temperature not specified in the publication
1300
sucrose
-
mutant N21S, pH 4.8, 50°C
5000
sucrose
-
mutant W19Y/N21S, pH 4.8, 50°C
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
Km-values of soluble and micelle entrapped enzyme
-
additional information
additional information
-
enzyme in cells immobilized in gelatin hydrogels are analyzed concerning kinetics, thermostability, and reusability, overview
-
additional information
additional information
-
kinetics, isozymes SAI and CWI, isozyme Sai has a lower KM for sucrose and a higher KM for raffinose compared to isozyme CWI
-
additional information
sucrose
-
0.23 mg/ml, pH and temperature not specified in the publication