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3.1.26.3: ribonuclease III

This is an abbreviated version!
For detailed information about ribonuclease III, go to the full flat file.

Word Map on EC 3.1.26.3

Reaction

Endonucleolytic cleavage to a 5'-phosphomonoester =

Synonyms

Aa-RNase III, AbsB, At1g55140, At3g13740, AtRTL1, AtRTL2, AtRTL3, BB_0705, BCG-RNase III, Bm-RNase III, cgR_1959, class 1 RNase III, DCL1, DCL2, DCL3, DCL4, DCR-1, Dicer, Dicer 1, Dicer-1, Dicer-2, Dicer1, double strand-specific endoRNase, Drosha, dsRNA-specific class 1 RNase III-like endoribonuclease, E. coli RNase III, Ec-RNase III, endoribonuclease III, endoribonucleases III, exodeoxyribonuclease III, HCS protein, Hs-Dicer, Hs-Drosha, KREPB10, KREPB9, Lac-RNase III, mini-III, mitochondrial RNA precursor-processing endonuclease 1, More, mRPN1, nuclease, ribo-, D, p241, Pac1p, RBIV RNase III, ribonuclease D, ribonuclease III, ribonuclease III-like protein, ribonuclease type III, RNase D, RNase G, RNase III, RNase III domain A, RNase III double-stranded RNA endonuclease, RNase III endonuclease, RNase III-like protein, RNase O, RNase3, rnc, RNC1, RNC3, RNC4, rncS, RNT1, Rnt1p, Rnt1p RNase III, Sa-RNase III, Sc-Rnt1p, SmRNase III, Tb09.160.3050, Tb927.8.5700, Tm-RNase III, TNase III

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.3 ribonuclease III

Purification

Purification on EC 3.1.26.3 - ribonuclease III

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography. RNase III heterodimers also can be purified from the inclusion body
-
gel filtration analysis on a Superdex 75 10/30 column
mutant enzyme D44N
-
Ni sepharose high performance chromatography, Q high performance chromatography
Ni2+ affinity column
of the human immunodeficiency virus type 1 reverse transcriptase
-
of the native and recombinant protein
of the overexpressed protein
-
of the overexpressed protein in yeast
-
partially
-
partially RNase O
-
purified by affinity chromatography on amylose-coupled agarose resin, recombinant RNC1 binds RNA but lacks endonuclease activity
purified on a Ni2+-NTA superflow column
-
recombinant C-terminally His6-tagged enzyme from Escherichia coli BL21(DE3) Codon Plus by metal affinity chromatography
-
recombinant enzyme using His-tag
recombinant His-tagged enzyme to over 90% purity
-
recombinant His-tagged RNase III from Escherichia coli by nickel affinity chromatography
-
recombinant HIs-tagged wild-type and mutant enzymes
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)recArnc105 by nickel affinity chromatography and gel filtration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli, expression of the mutant enzymes in a two-hybrid system in Saccharomyces strains
-
recombinant His6-tagged wild-type and mutant Dicer-2 from Sf21 insect cells by nickel affinity and heparin affinity chromatography, followed by gel filtration
-
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)recA,rnc105 by nickel affinity chromatography, the tag is cleaved off by thrombin
recombinant hybrid proteins to homogeneity
recombinant intein-tagged enzyme from Escherichia coli, to homogeneity, intein-tag is cleaved off
Paramecium bursaria Chlorella virus-1
-
recombinant mutant enzymes
-
recombinant protein
recombinant protein using His-tag
recombinant tagged heterodimer and homodimers from strain BL21 to homogeneity
-
recombinant wild-type and mutant GST-tagged enzymes from Escherichia coli strain Bl21(DE3) by glutathione affinity chromatography, tag cleavage by thrombin, and 4-aminobenzamidine affinity chromatography to remove thrombin, followed by gel filtration, to homogeneity
six-His fusion protein is purified from the soluble fraction using Ni-nitrilotriacetic resin
to homogeneity