3.1.26.3: ribonuclease III
This is an abbreviated version!
For detailed information about ribonuclease III, go to the full flat file.
Word Map on EC 3.1.26.3
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3.1.26.3
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rnai
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argonaute
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drosophila
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germline
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rna-induced
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viruses
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duplex
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ovarian
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microprocessor
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pri-mirnas
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single-stranded
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chromatin
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elegans
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helicase
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tumour
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neoplasm
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predisposition
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thyroid
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tumorigenesis
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rna-dependent
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sequence-specific
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stem-loop
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3'utrs
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cystic
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hotspot
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rhabdomyosarcoma
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heterochromatin
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dsrna-binding
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snornas
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retrotransposons
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exoribonuclease
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piwi-interacting
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gene-silencing
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endonucleolytic
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rna-directed
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virus-derived
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mirna-mediated
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multinodular
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overhang
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wilms
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rnai-based
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goiter
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hamartoma
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microrna-mediated
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let-7a
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exportin
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dead-box
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rna-processing
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pre-rrnas
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mirna-based
- 3.1.26.3
- rnai
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argonaute
- drosophila
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germline
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rna-induced
- viruses
- duplex
- ovarian
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microprocessor
-
pri-mirnas
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single-stranded
- chromatin
- elegans
- helicase
- tumour
- neoplasm
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predisposition
- thyroid
- tumorigenesis
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rna-dependent
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sequence-specific
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stem-loop
-
3'utrs
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cystic
-
hotspot
- rhabdomyosarcoma
- heterochromatin
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dsrna-binding
- snornas
-
retrotransposons
- exoribonuclease
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piwi-interacting
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gene-silencing
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endonucleolytic
-
rna-directed
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virus-derived
-
mirna-mediated
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multinodular
-
overhang
-
wilms
-
rnai-based
- goiter
- hamartoma
-
microrna-mediated
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let-7a
-
exportin
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dead-box
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rna-processing
- pre-rrnas
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mirna-based
Reaction
Endonucleolytic cleavage to a 5'-phosphomonoester =
Synonyms
Aa-RNase III, AbsB, At1g55140, At3g13740, AtRTL1, AtRTL2, AtRTL3, BB_0705, BCG-RNase III, Bm-RNase III, cgR_1959, class 1 RNase III, DCL1, DCL2, DCL3, DCL4, DCR-1, Dicer, Dicer 1, Dicer-1, Dicer-2, Dicer1, double strand-specific endoRNase, Drosha, dsRNA-specific class 1 RNase III-like endoribonuclease, E. coli RNase III, Ec-RNase III, endoribonuclease III, endoribonucleases III, exodeoxyribonuclease III, HCS protein, Hs-Dicer, Hs-Drosha, KREPB10, KREPB9, Lac-RNase III, mini-III, mitochondrial RNA precursor-processing endonuclease 1, More, mRPN1, nuclease, ribo-, D, p241, Pac1p, RBIV RNase III, ribonuclease D, ribonuclease III, ribonuclease III-like protein, ribonuclease type III, RNase D, RNase G, RNase III, RNase III domain A, RNase III double-stranded RNA endonuclease, RNase III endonuclease, RNase III-like protein, RNase O, RNase3, rnc, RNC1, RNC3, RNC4, rncS, RNT1, Rnt1p, Rnt1p RNase III, Sa-RNase III, Sc-Rnt1p, SmRNase III, Tb09.160.3050, Tb927.8.5700, Tm-RNase III, TNase III
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3.1.26.3 ribonuclease IIIAdvanced search results
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results (Engineering
Engineering on EC 3.1.26.3 - ribonuclease III
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D44N
E110A
uncoupling of the dsRNA-binding and processing abilities of the enzyme
E110K
E110Q
Q157A
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is a conserved glutamine in the Aa-RNase III dsRNA-binding domain. Aa-RNase III cleavage of the pre-16S substrate is blocked by the Q157A mutation, which reflects a loss of substrate binding affinity. But the Q157A mutation does not affect folding or structure in a significant manner
S300A
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site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme
S300A/S302A
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site-directed mutagenesis, the double mutation completely disrupts nuclear localization
S300E
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site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme
S300E/S302D
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site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme
S302A
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site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme
S302D
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site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme
D114A
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mutant exhibits catalytic activity in vitro in 10 mM Mg2+ buffer that is comparable to that of the wild-type enzyme. At 1 mM Mg2+, the activity is significantly lower, KM-value for Mg2+ is about 2.8fold larger than the wild-type value
D128A
site-directed mutagenesis, the D128A mutation in both RNase III subunits, D128A/D128'A, causes an 83fold increase in KD in the interaction of RNase III with YmdB
D45A
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mutant enzyme exhibits negligible activity, regardless of the Mg2+ concentration
D45E
D45N
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mutant enzyme exhibits negligible activity, regardless of the Mg2+ concentration
E100A
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mutant enzyme requires higher Mg2+ concentrations for optimal activity than the wild-type enzyme
E117D
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site-directed mutagenesis, mutant exhibits normal homodimeric behaviour, can bind substrates but shows highly reduced hydrolysis activity compared to the wild-type enzyme
E117Q
E38A
E41A
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mutant exhibits catalytic activity in vitro in 10 mM Mg2+ buffer that is comparable to that of the wild-type enzyme. At 1 mM Mg2+, the activity is significantly lower, KM-value for Mg2+ is about 2.8fold larger than the wild-type value
E41A/D114A
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KM-value for Mg2+ is about 85fold larger than the wild-type value
E65A
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mutant enzyme requires higher Mg2+ concentrations for optimal activity than the wild-type enzyme
Q153P
the Q153P substitution in the middle of the flexible linker between the endoND and the dsRBD abolish RNA-cleavage activity
S195A/S198A
site-directed mutagenesis, the mutant shows a slightly reduced phosphorylation level compared to wild-type
S33E/S34E
site-directed mutagenesis of phosphorylation sites, molecular dynamic simulations of the S33E/S34E double mutant, which formally provides the same double-negative charge as a single S33 or S34 phosphomonoester, indicate that an additional acidic residue at position 34 does not provide a stabilized interaction with R95. In contrast to the bidentate pS33-R95 side chain interaction, the observed salt bridge consists of a monodentate engagement of R95 with the E33 side chain, and no involvement of the E34 side chain. The S33E/S34E mutant shows abolished phosphorylation and cleaves R1.1 RNA with an efficiency comparable to, but not greater than unphosphorylated RNase III. The S33A/S34A double mutant is essentially fully resistant to phosphorylation
D48A
site-directed mutagenesis, catalytically inactive mutant
E44A
site-directed mutagenesis, catalytically inactive mutant
D48A
Mycobacterium tuberculosis variant bovis Pasteur 1173P2
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site-directed mutagenesis, catalytically inactive mutant
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E44A
Mycobacterium tuberculosis variant bovis Pasteur 1173P2
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site-directed mutagenesis, catalytically inactive mutant
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K371A
dissociation constant for RNA is 2.1fold higher than the wild-type value
M368A
dissociation constant for RNA is 1.4fold higher than the wild-type value
M368E
dissociation constant for RNA is nearly identical to wild-type value
R372A
dissociation constant for RNA is nearly identical to wild-type value
S376E
dissociation constant for RNA is 1.3fold higher than the wild-type value
E125A
site-directed mutagenesis, the mutation does not compromise wild-type enzyme SmRNase III dimerization ability
E125Q
site-directed mutagenesis, mutation does not compromise wild-type enzyme SmRNase III dimerization ability
D63A
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site-directed mutagenesis, analysis of binding specificity and target sites compared to the wild-type enzyme
E135A
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site-directed mutagenesis, analysis of binding specificity and target sites compared to the wild-type enzyme
D63A
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site-directed mutagenesis, analysis of binding specificity and target sites compared to the wild-type enzyme
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E135A
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site-directed mutagenesis, analysis of binding specificity and target sites compared to the wild-type enzyme
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D70A
G238R
site-directed mutagenesis of KREPB10, the mutant has reduced steady-state levels compared to wild-type KREPB10
G238V
site-directed mutagenesis of KREPB10, the mutant has reduced steady-state levels compared to wild-type KREPB10
G270R
site-directed mutagenesis of KREPB9, the G270R mutant protein is considerably weaker due to the lower steady-state level
G270V
site-directed mutagenesis of KREPB9, mutant does not appear to shift compared to the wild-type
G238R
Trypanosoma brucei 927/4 GUTat10.1
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site-directed mutagenesis of KREPB10, the mutant has reduced steady-state levels compared to wild-type KREPB10
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G238V
Trypanosoma brucei 927/4 GUTat10.1
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site-directed mutagenesis of KREPB10, the mutant has reduced steady-state levels compared to wild-type KREPB10
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G270R
Trypanosoma brucei 927/4 GUTat10.1
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site-directed mutagenesis of KREPB9, the G270R mutant protein is considerably weaker due to the lower steady-state level
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G270V
Trypanosoma brucei 927/4 GUTat10.1
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site-directed mutagenesis of KREPB9, mutant does not appear to shift compared to the wild-type
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additional information
D44N
site-directed mutagenesis, the mutation does not fully inactivate the enzyme, and dsRNA cleavage occurs during crystallization of the mutant enzyme
E110K
loss of Mg2+ binding capacity, non-functional, uncoupling of the dsRNA-binding and processing abilities of the enzyme
E110Q
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mutant enzyme has negligible RNA cleavage activity but retains its RNA binding affinity
E110Q
uncoupling of the dsRNA-binding and processing abilities of the enzyme
D45E
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mutant enzyme exhibits negligible activity, regardless of the Mg2+ concentration
E117Q
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mutant enzyme can still bind to the substrate RNA in presence of Mg2+ but cannot cleave it
E117Q
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site-directed mutagenesis, mutant exhibits normal homodimeric behaviour, can bind substrates but is unable to cleave the substrates
E38A
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mutant enzyme requires higher Mg2+ concentrations for optimal activity than the wild-type enzyme
E38A
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single amino acid substitution, preventing cleavage at the secondary site. RNase III(E38A) generates discrete-sized products
D70A
constructed point mutation, abolishes the catalytic activity of the protein but not its ability to bind to RNA substrates
D70A
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constructed point mutation, abolishes the catalytic activity of the protein but not its ability to bind to RNA substrates
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additional information
generation of null mutants for both genes RNC3 and RNC4 from the T-DNA insertion collection, termed rnc3-1 and rnc4-1, respectively, and of a double knockout mutant rnc3/4. Phenotypes, overview. rRNA deficiencies observed in rnc3/4 can be complemented by an RNC transgene
additional information
generation of null mutants for both genes RNC3 and RNC4 from the T-DNA insertion collection, termed rnc3-1 and rnc4-1, respectively, and of a double knockout mutant rnc3/4. Phenotypes, overview. rRNA deficiencies observed in rnc3/4 can be complemented by an RNC transgene
additional information
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generation of null mutants for both genes RNC3 and RNC4 from the T-DNA insertion collection, termed rnc3-1 and rnc4-1, respectively, and of a double knockout mutant rnc3/4. Phenotypes, overview. rRNA deficiencies observed in rnc3/4 can be complemented by an RNC transgene
additional information
DELTAmrnC strain has no major difference in growth rate compared with wild-type, but reaches a slightly lower saturation density
additional information
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DELTAmrnC strain has no major difference in growth rate compared with wild-type, but reaches a slightly lower saturation density
additional information
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several pnp alleles constructed. Deletion DELTApnpL1001, which removes the upper (central) part of the large stem-loop (SL1) that serves as a substrate for RNase III
additional information
generation of an rnc null mutant in Borrelia burgdorferi that exhibits a pleiotropic phenotype, including decreased growth rate and increased cell length. The chromosomal rncBb gene is replaced through homologous recombination with the gentamicin resistance cassette flgBp-aacC1. Mutant rncBb operon structure. The RNase III mutant is viable but has altered processing of rRNA, phenotype, overview
additional information
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generation of an rnc null mutant in Borrelia burgdorferi that exhibits a pleiotropic phenotype, including decreased growth rate and increased cell length. The chromosomal rncBb gene is replaced through homologous recombination with the gentamicin resistance cassette flgBp-aacC1. Mutant rncBb operon structure. The RNase III mutant is viable but has altered processing of rRNA, phenotype, overview
additional information
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dcr-1 gene N-terminal deletion and null mutants show defective RNAi and are steril, overview
additional information
generation of a deletion mutant of gene cgR_1959, the DELTArnc mutant. Deletion of the rnc gene encoding RNase III results in cell elongation in Corynebacterium glutamicum strain R. Microarray analysis and quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis show that the level of mraZ mRNA increases, whereas cgR_1596 and ftsEX mRNA are decreased in the DELTArnc mutant. RNase III cleaves the coding region of mraZ mRNA. Deletion of cgR_1596 caused a defect in cell separation. Phenotype, overview
additional information
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generation of a deletion mutant of gene cgR_1959, the DELTArnc mutant. Deletion of the rnc gene encoding RNase III results in cell elongation in Corynebacterium glutamicum strain R. Microarray analysis and quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis show that the level of mraZ mRNA increases, whereas cgR_1596 and ftsEX mRNA are decreased in the DELTArnc mutant. RNase III cleaves the coding region of mraZ mRNA. Deletion of cgR_1596 caused a defect in cell separation. Phenotype, overview
additional information
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construction of a heterodimer comprising one functional wild-type subunit and one inactive E117Q mutant subunit, which carries the E117Q mutation allowing the mutant subunit to bind but not cleave the substrate, the functional subunit is sufficient for catalytic activity of the heterodimer
additional information
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construction of a mutant enzyme RNase III[DELTAdsRBD] lacking the dsRNA binding domain, the mutant is still catalytically active at low salt concentrations in presence of either 25 mM Mg2+ or 5 mM Mn2+ with slightly reduced catalytic efficiency, but unaltered substrate specificity
additional information
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construction of hybrid proteins consisiting of the N-terminal nuclease domain of Rhodobacter capsulatus and the C-terminal dsRNA-binding domain of Escherichia coli and vice versa, extension of the spacer region between the N-terminal and C-terminal domains does not alter the cleavage specificity
additional information
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the stability of rpoS mRNA, and concomitantly the concentration of deltaS, are significantly higher in an RNase III-deficient mutant. Investigation of the dsrA mutant (rnc+dsrA-) and its isogenic variant lacking functional RNase III (rnc-dsrA-)
additional information
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construction of mutant strain MG1655rnc-14::DELTATn10
additional information
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construction of strain MG1655 rnc-14::DELTATn10 from wild-type straiin HT115
additional information
sequencing RNA from both an RNase III mutant (SK4455, rnc-14::DELTATn10 thyA715 rph-1) and the parental strain (MG1693, thyA715 rph-1) from which an RNase III mutant is derived
additional information
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construction of strain MG1655 rnc-14::DELTATn10 from wild-type straiin HT115
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additional information
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construction of mutant strain MG1655rnc-14::DELTATn10
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additional information
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sequencing RNA from both an RNase III mutant (SK4455, rnc-14::DELTATn10 thyA715 rph-1) and the parental strain (MG1693, thyA715 rph-1) from which an RNase III mutant is derived
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additional information
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construction of mutant enzymes containing inserts encoding the enzyme's heparin binding region HBR1, HBR2, or HBR3 by site-directed mutagenesis, cytotoxicity of wild-type and mutant enzymes to Beas-2B cells, overview
additional information
an artificial small RNA (asRNA), composed of a Dicer-binding RNA element and an antisense RNA, can be used to induce Dicer to process and degrade a specific RNA. Development of a method which is named DICERi for gene silencing or RNA editing. To prove the feasibility of asRNA, MALAT-1 is selected as target and Hela and MDA-MB-231 cells are used as experimental models. The results of qRT-PCR show that the introduction of asRNA decreases the relative expression level of target gene significantly. Cell proliferation and cell migration are both suppressed remarkably after asRNA is expressed in Hela and MDA-MB-231 cells. T gene silencing effects were caused by Dicer. When the cleavage role of Dicer is silenced, the relative expression level of MALAT-1 is not affected after the introduction of asRNA. The effect of asRNA is dependent on Dicer. Method evaluation, overview
additional information
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construction of hybrid proteins consisting of the N-terminal nuclease domain of Rhodobacter capsulatus and the C-terminal dsRNA-binding domain of Escherichia coli and vice versa
additional information
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construction of deletion mutants RNT1DELTA2-329 and RNT1DELTA2-198, which are both catalytically active in vitro but do not rescue a growth defective mutant and are not able to retain activity and viability in vivo, construction of a AGNN-loop exchange mutant GNRA-loop shows reduced activity and substrate selectivity
additional information
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construction of several deletion mutants, lacking parts or total of the C-terminus or N-terminus, deletion of the N-terminal domain leads to slight accumulation of unprocessed 25S pre-rRNA in vivo and reduced enzyme activity in vitro
additional information
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building a class of RNA sensing actuation devices based on direct integration of RNA aptamers into a region of the RNase III Rnt1p hairpin that modulates Rnt1p cleavage rates, design of an Rnt1p switch platform based on direct replacement of the CEB with an aptamer sequence. Integration of a sensor component, DELTATCT-4 aptamer, into the actuator component, R31L-3B4Inv Rnt1p hairpin. Ligand binding to the integrated aptamer domain is associated with a structural change sufficient to inhibit Rnt1p processing, overview. Three tuning strategies based on the incorporation of different functional modules into the Rnt1p switch platform optimize switch dynamics and ligand responsiveness. Application of multiple switch modules decreases theophylline responsiveness and increases fold-change. The tuning modules can be implemented combinatorially in a predictable manner to further improve the regulatory response properties of the switch. The modularity and tunability of the Rnt1p switch platform will allow for rapid optimization and tailoring of this gene control device. Method evaluation and system stabililty, overview
additional information
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construction of an enzyme deficient mutant which has a strongly reduced growth rate compared with the wild type, the sRNA MicA is found to be extremely stable in the deficiency mutant
additional information
an enzyme deletion mutant SmDELTArnc shows a symbiotic phenotype. Plants inoculated with the wild-type and complemented strains developed significantly longer shoots than those inoculated with the mutant bacteria, SmDELTArnc or SmDELTArnc (pSRK), which are similar to those of the control mock-treated plants
additional information
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an enzyme deletion mutant SmDELTArnc shows a symbiotic phenotype. Plants inoculated with the wild-type and complemented strains developed significantly longer shoots than those inoculated with the mutant bacteria, SmDELTArnc or SmDELTArnc (pSRK), which are similar to those of the control mock-treated plants
additional information
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rnc mutant (obtained by homologous recombination) is viable. Deletion of the rnc gene in Staphylococcus aureus does not affect cell growth in rich medium
additional information
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construction of an RNase III inactivation mutant DELTArnc from Staphylococcus aureus strain 8325-4. The DELTArnc strain shows reduced extracellular protein levels and is less pathogenic compared with its parent strain
additional information
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effect of mutations in the catalytic site of Staphylococcus aureus RNase III, overview
additional information
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construction of an RNase III inactivation mutant DELTArnc from Staphylococcus aureus strain 8325-4. The DELTArnc strain shows reduced extracellular protein levels and is less pathogenic compared with its parent strain
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additional information
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effect of mutations in the catalytic site of Staphylococcus aureus RNase III, overview
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additional information
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creation of an RNase III null mutant of Streptococcus pyogenes by RNase III gene deletion
additional information
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creation of an RNase III null mutant of Streptococcus pyogenes by RNase III gene deletion
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additional information
Streptococcus pyogenes serotype 14
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creation of an RNase III null mutant of Streptococcus pyogenes by RNase III gene deletion
additional information
Streptococcus pyogenes serotype 14 HSC5
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creation of an RNase III null mutant of Streptococcus pyogenes by RNase III gene deletion
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additional information
construction of a complemented DELTArnc strain
additional information
Streptococcus pyogenes serotype M1 SF370 (M1GAS)
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construction of a complemented DELTArnc strain
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additional information
generation of a disruption mutant of the chromosomal RNase III gene rnc by insertional mutagenesis, the mutant strain shows reduced actinomycin production. Complementation of mutant strain JSE1980 with pJSE1995 encoding the wild-type rnc gene restores actinomycin production to nearly wild-type levels
additional information
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generation of a disruption mutant of the chromosomal RNase III gene rnc by insertional mutagenesis, the mutant strain shows reduced actinomycin production. Complementation of mutant strain JSE1980 with pJSE1995 encoding the wild-type rnc gene restores actinomycin production to nearly wild-type levels
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additional information
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constructed RNase III null mutant, phenotypic analysis
additional information
rnc null mutant of Streptomyces coelicolor M145 does not produce actinorhodin or undecylprodigiosin. The strain bearing the disrupted rnc gene was designated JSE1880
additional information
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rnc null mutant of Streptomyces coelicolor M145 does not produce actinorhodin or undecylprodigiosin. The strain bearing the disrupted rnc gene was designated JSE1880
additional information
generation of the RNase III-deletion strain derivative M145 rnc::aac(3)IV, JSE1880 rnc-mutant strain, comparison of gene expression between the Streptomyces coelicolor M145 wild-type strain and the JSE1880 rnc-mutant strain. In silico search for sRNA genes adjacent to mRNAs that are upregulated in rnc mutant, detailed overview
additional information
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generation of the RNase III-deletion strain derivative M145 rnc::aac(3)IV, JSE1880 rnc-mutant strain, comparison of gene expression between the Streptomyces coelicolor M145 wild-type strain and the JSE1880 rnc-mutant strain. In silico search for sRNA genes adjacent to mRNAs that are upregulated in rnc mutant, detailed overview
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additional information
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generation of the RNase III-deletion strain derivative M145 rnc::aac(3)IV, JSE1880 rnc-mutant strain, comparison of gene expression between the Streptomyces coelicolor M145 wild-type strain and the JSE1880 rnc-mutant strain. In silico search for sRNA genes adjacent to mRNAs that are upregulated in rnc mutant, detailed overview
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additional information
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generation of the RNase III-deletion strain derivative M145 rnc::aac(3)IV, JSE1880 rnc-mutant strain, comparison of gene expression between the Streptomyces coelicolor M145 wild-type strain and the JSE1880 rnc-mutant strain. In silico search for sRNA genes adjacent to mRNAs that are upregulated in rnc mutant, detailed overview
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additional information
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rnc null mutant of Streptomyces coelicolor M145 does not produce actinorhodin or undecylprodigiosin. The strain bearing the disrupted rnc gene was designated JSE1880
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additional information
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the catalytically inactive mutant RNase3-Ala can bind the substrates like 22 nt ds-siRNA or 60 bp dsRNA, formation of high-molecular-mass RNA-protein complexes
additional information
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inducible knockdown of mRPN1 in Trypanosoma brucei results in loss of gRNA and accumulation of precursor transcripts, consistent with a role of mRPN1 in processing
additional information
generation of KREPB9 and KREPB10 null mutants. KREPB9 null cells show negligible differences in growth compared to parental 427 wild-type cells and single-knockout cells that retain KREPB9 expression. In KREPB10 null cells, growth is identical to single-knockout cells that retain KREPB10 expression, negligible differences from 427 wild-type cells
additional information
generation of KREPB9 and KREPB10 null mutants. KREPB9 null cells show negligible differences in growth compared to parental 427 wild-type cells and single-knockout cells that retain KREPB9 expression. In KREPB10 null cells, growth is identical to single-knockout cells that retain KREPB10 expression, negligible differences from 427 wild-type cells
additional information
Trypanosoma brucei 927/4 GUTat10.1
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generation of KREPB9 and KREPB10 null mutants. KREPB9 null cells show negligible differences in growth compared to parental 427 wild-type cells and single-knockout cells that retain KREPB9 expression. In KREPB10 null cells, growth is identical to single-knockout cells that retain KREPB10 expression, negligible differences from 427 wild-type cells
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