Information on EC 3.1.26.3 - ribonuclease III

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY
3.1.26.3
-
RECOMMENDED NAME
GeneOntology No.
ribonuclease III
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Aa-RNase III
-
-
Aa-RNase III
O67082
-
AtRTL3
-
protein not detected in any of the tissues studied, possibly non-functional gene sequence
Dicer
-
-
Dicer
-
Dicer contains two RNase III domains (RNase IIIa and RNase IIIb)
Dicer
Q9UPY3
-
Dicer
Q9UPY3
Dicer protein contains two RNase III domains (RNase IIIa and RNase IIIb)
Dicer
-
-
Dicer
-
enzyme complex containing RNAse III enzyme
double strand-specific endoRNase
Q9ZBQ7
-
double strand-specific endoRNase
Streptomyces coelicolor M145
Q9ZBQ7
-
-
Drosha
Q9NRR4
-
E. coli RNase III
-
-
Ec-RNase III
P0A7Y0
-
Ec-RNase III
Escherichia coli K12
P0A7Y0
-
-
endoribonuclease III
-
-
endoribonuclease III
-
-
exodeoxyribonuclease III
-
-
HCS protein
-
-
-
-
Hs-Dicer
Q9UPY3
-
Hs-Drosha
Q9NRR4
-
Lac-RNase III
-
-
mini-III
-
a fourth class of RNase III, consisting only of the RNase III catalytic domain and functioning in the maturation of the 23S rRNA in Bacillus subtilis
mini-III
O31418
unusual member of the RNase III family of enzymes, catalyses 23S ribosomal RNA maturation in Bacillus subtilis. Defines a fourth class of naturally occurring functional RNase III enzymes, which consists of just the catalytic domain
nuclease, ribo-, D
-
-
-
-
p241
-
-
-
-
RBIV RNase III
Q5YF04
-
RBIV RNase III
Rock bream iridovirus RBIV
Q5YF04
-
-
ribonuclease D
-
-
-
-
ribonuclease D
-
-
ribonuclease III
-
-
ribonuclease III
O67082
-
ribonuclease III
-
-
ribonuclease III
-
-
ribonuclease III
-
-
ribonuclease III
-
double-strand-specific endoribonuclease
ribonuclease III
-
-
ribonuclease III
Q9NRR4, Q9UPY3
-
ribonuclease III
Q02555
-
ribonuclease III
-
-
ribonuclease III
Q9X0I6
-
ribonuclease III
A6YSL1
-
ribonuclease III-like protein
Q67B98
-
RNase D
-
-
-
-
RNase D
-
-
RNase G
P0A9J0
-
RNase III
O67082
-
RNase III
-
-
RNase III
-
-
RNase III
-
-
RNase III
P0A7Y0
-
RNase III
Escherichia coli K12
P0A7Y0
-
-
RNase III
Q9NRR4, Q9UPY3
-
RNase III
-
-
RNase III
-
-
RNase III
Paramecium bursaria Chlorella virus-1
-
-
RNase III
Q5YF04
-
RNase III
Rock bream iridovirus RBIV
Q5YF04
-
-
RNase III
Staphylococcus aureus 8325-4
-
-
-
RNase III
Streptococcus pyogenes SF370
-
-
-
RNase III
Streptomyces coelicolor M145
Q9ZBQ7
-
-
RNase III
-
-
RNase III
-
-
RNase III
Q9X0I6
-
RNase III
-
-
RNase III
A6YSL1
-
RNase III double-stranded RNA endonuclease
-
-
RNase III endonuclease
-
-
RNase O
-
-
-
-
RNase O
-
-
RNC1
A6YSL1
plant specific protein that contains two RNase III domains
Rnt1p RNase III
-
-
Sa-RNase III
-
-
Sc-Rnt1p
Q02555
-
Tm-RNase III
-
-
Tm-RNase III
Q9X0I6
-
mitochondrial RNA precursor-processing endonuclease 1
-
-
additional information
-
-
-
additional information
-
enzyme belongs to the ribonuclease III superfamily
additional information
-
the enzyme belongs to the double-stranded RNA specific RNase III family of endoribonucleases
additional information
-
the enzyme belongs to the RNase III superfamily
additional information
-
the enzyme belongs to the double-stranded RNA specific RNase III family of endoribonucleases
CAS REGISTRY NUMBER
COMMENTARY
78413-14-6
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
class 1 RNase III enzyme
-
-
Manually annotated by BRENDA team
prokaryotic RNases III are class I enzymes containing a single endonuclease domain linked to a double-stranded RNA binding domain
-
-
Manually annotated by BRENDA team
Columbia Col 0 ecotype, three different proteins named AtRTL1-AtRTL3
-
-
Manually annotated by BRENDA team
Dicer, a class III RNase III containing an N-terminal ATP-dependent RNA helicase domain, a PAZ motif, and 2 C-terminal endonuclease domains followed by a single dsRNA binding domain
-
-
Manually annotated by BRENDA team
strain W168 and strain yazCdd (SSB1044)
UniProt
Manually annotated by BRENDA team
several strains used
-
-
Manually annotated by BRENDA team
Dicer, a class III RNase III containing an N-terminal ATP-dependent RNA helicase domain, a PAZ motif, and 2 C-terminal endonuclease domains followed by a single dsRNA binding domain
-
-
Manually annotated by BRENDA team
Dicer, a class III RNase III containing an N-terminal ATP-dependent RNA helicase domain, a PAZ motif, and 2 C-terminal endonuclease domains followed by a single dsRNA binding domain
-
-
Manually annotated by BRENDA team
; K12
SwissProt
Manually annotated by BRENDA team
class 1 RNase III enzyme
-
-
Manually annotated by BRENDA team
gene rnc, strain JM109, recombinant hybrid enzymes consisiting of the N-terminal nuclease domain of Rhodobacter capsulatus and the C-terminal dsRNA-binding domain of Escherichia coli, and vice versa
-
-
Manually annotated by BRENDA team
mutant strains AB301,105, TS241 RNase III deficient
-
-
Manually annotated by BRENDA team
prokaryotic RNases III are class I enzymes containing a single endonuclease domain linked to a double-stranded RNA binding domain
-
-
Manually annotated by BRENDA team
strain SDF204 and the isogenic RNase III (rnc) deficient strain SDF205
-
-
Manually annotated by BRENDA team
Escherichia coli K12
K12
SwissProt
Manually annotated by BRENDA team
class 3 RNase III enzyme
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
class 2 RNase III enzyme
-
-
Manually annotated by BRENDA team
Dicer, a class III RNase III containing an N-terminal ATP-dependent RNA helicase domain, a PAZ motif, and 2 C-terminal endonuclease domains followed by a single dsRNA binding domain
-
-
Manually annotated by BRENDA team
alphaPax6cre mice
-
-
Manually annotated by BRENDA team
Paramecium bursaria Chlorella virus-1
i.e. PBCV-1, gene a464r
-
-
Manually annotated by BRENDA team
identification of a novel rat mRNA sequence that is highly homologous to human ribonuclease III by differential display
SwissProt
Manually annotated by BRENDA team
gene rnc, strain 37b4, recombinant hybrid enzymes consisiting of the N-terminal nuclease domain of Rhodobacter capsulatus and the C-terminal dsRNA-binding domain of Escherichia coli, and vice versa
-
-
Manually annotated by BRENDA team
Rock bream iridovirus RBIV
RBIV
UniProt
Manually annotated by BRENDA team
class 1 RNase III enzyme
-
-
Manually annotated by BRENDA team
serovar Typhimurium strain SL1344
-
-
Manually annotated by BRENDA team
strain SL1344 and derivatives thereof
-
-
Manually annotated by BRENDA team
Staphylococcus aureus 8325-4
-
-
-
Manually annotated by BRENDA team
M1 serotype
-
-
Manually annotated by BRENDA team
Streptococcus pyogenes SF370
M1 serotype
-
-
Manually annotated by BRENDA team
J15501 wild-type strain and C120 absB mutant strain
UniProt
Manually annotated by BRENDA team
Streptomyces coelicolor M145
M145
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.083
-
515 bp dsRNA
-
wild-type Dicer-2, pH 7.4, 25C
0
0.33
-
515 bp dsRNA
-
wild-type Dicer-2 in presence of protein R2D2 at equimolar level, pH 7.4, 25C
0
1.33
-
515 bp dsRNA
-
wild-type Dicer-2 in presence of Loquacious-PB at equimolar level, pH 7.4, 25C
0
466.7
-
Aa-[16S[micro-hp]RNA]
-
pH 8.0, 40C
0
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0017
-
Ethidium bromide
-
pH 8.0, 37C
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.014
-
2-hydroxyisoquinoline-1,3-dione
-
inhibits cleavage of R1.1 RNA, IC50: 0.014 mM for Mg2+-supported reaction, 0.008 mM for Mn2+-suppoeted reaction, noncompetitive inhibition
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
gel mobility shift assay for substrate bindng determination
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
9
-
and above, activity range
6.9
7.4
-
-
7.6
9.75
-
-
PDB
SCOP
CATH
ORGANISM
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Campylobacter jejuni subsp. jejuni (strain IA3902)
Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phosphoprotein
-
two phosphorylation sites at Ser300 and Ser302, which locates Drosha to the nucleus
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, 25 mM Tris, pH 8, 8% (NH4)2SO4, 50% glycerol, several months, no loss of activity
-
-70C, 1.3 M NH4Cl, 2 years, 40-50% loss of activity
-
-20C, purified recombinant non-tagged enzyme, over one year without loss of activity
Paramecium bursaria Chlorella virus-1
-
-20C, 40 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM EDTA, 5 mM 2-mercaptoethanol, 50% glycerol
A6YSL1, -
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
the Ascovirus-encoded RNase III autoregulates its expression and suppresses RNA interference-mediated gene silencing
-
Micro-drosha siRNAs-1 to -3 yield a very highly significant level of target transcript knockdown compared with the control siRNA following egg-stage soaks, with a mean 88.1%, 98.6%, and 99.89% decrease, respectively. Soaking the J2s in dicer siRNA results in a modest decrease in dicer transcript abundance
-
Micro-dicer siRNAs-1 to -3 elicit percentage transcript increases of 775, 1832 and 645, respectively
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
drosha, pasha and their ancillary factors may represent excellent targets for novel nematicides and/or in planta controls, and potentially other parasitic nematodes, through disruption of micro-RNA-directed developmental pathways