Literature summary for 3.1.26.3 extracted from

Zamore, P.D.
Thirty-three years later, a glimpse at the ribonuclease III active site (2001), Mol. Cell, 8, 1158-1160.

Search for more literature for 3.1.26.3

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	divalent metal ion requirement for catalysis	Drosophila melanogaster
Mg2+	divalent metal ion requirement for catalysis	Escherichia coli
Mg2+	divalent metal ion requirement for catalysis	Homo sapiens
Mg2+	divalent metal ion requirement for catalysis	Arabidopsis thaliana
Mg2+	divalent metal ion requirement for catalysis	Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dsRNA + H2O	Aquifex aeolicus	reshaping of fully or partially double-stranded RNA precursors in to mature RNAs involved in pre-mRNA splicing, RNA modification, translation, gene silencing, and regulation of developmental timing	mature RNA	-	?
dsRNA + H2O	Drosophila melanogaster	reshaping of fully or partially double-stranded RNA precursors into mature RNAs involved in pre-mRNA splicing, RNA modification, translation, gene silencing, and regulation of developmental timing	mature RNA	-	?
dsRNA + H2O	Escherichia coli	reshaping of fully or partially double-stranded RNA precursors into mature RNAs involved in pre-mRNA splicing, RNA modification, translation, gene silencing, and regulation of developmental timing	mature RNA	-	?
dsRNA + H2O	Homo sapiens	reshaping of fully or partially double-stranded RNA precursors into mature RNAs involved in pre-mRNA splicing, RNA modification, translation, gene silencing, and regulation of developmental timing	mature RNA	-	?
dsRNA + H2O	Arabidopsis thaliana	reshaping of fully or partially double-stranded RNA precursors into mature RNAs involved in pre-mRNA splicing, RNA modification, translation, gene silencing, and regulation of developmental timing, enzyme is required for the orderly progression of plant development and for the defense of eukaryotic parasitic DNA and viruses	mature RNA	-	?
dsRNA + H2O	Caenorhabditis elegans	reshaping of fully or partially double-stranded RNA precursors into mature RNAs involved in pre-mRNA splicing, RNA modification, translation, gene silencing, and regulation of developmental timing, enzyme is required for the orderly progression of plant development and for the defense of eukaryotic parasitic DNA and viruses	mature RNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Aquifex aeolicus	-	prokaryotic RNases III are class I enzymes containing a single endonuclease domain linked to a double-stranded RNA binding domain	-
Arabidopsis thaliana	-	Dicer, a class III RNase III containing an N-terminal ATP-dependent RNA helicase domain, a PAZ motif, and 2 C-terminal endonuclease domains followed by a single dsRNA binding domain	-
Caenorhabditis elegans	-	Dicer, a class III RNase III containing an N-terminal ATP-dependent RNA helicase domain, a PAZ motif, and 2 C-terminal endonuclease domains followed by a single dsRNA binding domain	-
Drosophila melanogaster	-	Dicer, a class III RNase III containing an N-terminal ATP-dependent RNA helicase domain, a PAZ motif, and 2 C-terminal endonuclease domains followed by a single dsRNA binding domain	-
Escherichia coli	-	prokaryotic RNases III are class I enzymes containing a single endonuclease domain linked to a double-stranded RNA binding domain	-
Homo sapiens	-	Dicer, a class III RNase III containing an N-terminal ATP-dependent RNA helicase domain, a PAZ motif, and 2 C-terminal endonuclease domains followed by a single dsRNA binding domain	-

Reaction

Reaction	Comment	Organism	Reaction ID
Endonucleolytic cleavage to a 5'-phosphomonoester	catalytic active site and substrate binding site structure and number determines the classification into classes I to III, overview	Drosophila melanogaster	a
Endonucleolytic cleavage to a 5'-phosphomonoester	catalytic active site and substrate binding site structure and number determines the classification into classes I to III, overview	Escherichia coli	a
Endonucleolytic cleavage to a 5'-phosphomonoester	catalytic active site and substrate binding site structure and number determines the classification into classes I to III, overview	Homo sapiens	a
Endonucleolytic cleavage to a 5'-phosphomonoester	catalytic active site and substrate binding site structure and number determines the classification into classes I to III, overview	Arabidopsis thaliana	a
Endonucleolytic cleavage to a 5'-phosphomonoester	catalytic active site and substrate binding site structure and number determines the classification into classes I to III, overview	Caenorhabditis elegans	a
Endonucleolytic cleavage to a 5'-phosphomonoester	catalytic active site and substrate binding site structure and number determines the classification into classes I to III, overview, mechanism	Aquifex aeolicus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dsRNA + H2O	reshaping of fully or partially double-stranded RNA precursors in to mature RNAs involved in pre-mRNA splicing, RNA modification, translation, gene silencing, and regulation of developmental timing	Aquifex aeolicus	mature RNA	-	?
dsRNA + H2O	reshaping of fully or partially double-stranded RNA precursors into mature RNAs involved in pre-mRNA splicing, RNA modification, translation, gene silencing, and regulation of developmental timing	Drosophila melanogaster	mature RNA	-	?
dsRNA + H2O	reshaping of fully or partially double-stranded RNA precursors into mature RNAs involved in pre-mRNA splicing, RNA modification, translation, gene silencing, and regulation of developmental timing	Escherichia coli	mature RNA	-	?
dsRNA + H2O	reshaping of fully or partially double-stranded RNA precursors into mature RNAs involved in pre-mRNA splicing, RNA modification, translation, gene silencing, and regulation of developmental timing	Homo sapiens	mature RNA	-	?
dsRNA + H2O	reshaping of fully or partially double-stranded RNA precursors into mature RNAs involved in pre-mRNA splicing, RNA modification, translation, gene silencing, and regulation of developmental timing, enzyme is required for the orderly progression of plant development and for the defense of eukaryotic parasitic DNA and viruses	Arabidopsis thaliana	mature RNA	-	?
dsRNA + H2O	reshaping of fully or partially double-stranded RNA precursors into mature RNAs involved in pre-mRNA splicing, RNA modification, translation, gene silencing, and regulation of developmental timing, enzyme is required for the orderly progression of plant development and for the defense of eukaryotic parasitic DNA and viruses	Caenorhabditis elegans	mature RNA	-	?
dsRNA + H2O	cleavage of fully or partially double-stranded RNA precursors into mature structural and catalytic RNAs such as the snRNAs that splice pre-mRNA, rRNAs, and tRNAs that function in translation, swnoRNAs that guide modificatin of rRNAs, and individual mRNAs, whose expression they regulate	Escherichia coli	mature RNA	staggered breaks with 2 nt, 3'-overhanging ends and 5'-phosphate and 3'-hydroxy termini	?
dsRNA + H2O	cleavage of fully or partially double-stranded RNA precursors into mature structural and catalytic RNAs such as the snRNAs that splice pre-mRNA, rRNAs, and tRNAs that function in translation, swnoRNAs that guide modificatin of rRNAs, and individual mRNAs, whose expression they regulate	Homo sapiens	mature RNA	staggered breaks with 2 nt, 3'-overhanging ends and 5'-phosphate and 3'-hydroxy termini	?
dsRNA + H2O	cleavage of fully or partially double-stranded RNA precursors into mature structural and catalytic RNAs such as the snRNAs that splice pre-mRNA, rRNAs, and tRNAs that function in translation, swnoRNAs that guide modification of rRNAs, and individual mRNAs, whose expression they regulate	Arabidopsis thaliana	mature RNA	-	?
dsRNA + H2O	cleavage of fully or partially double-stranded RNA precursors into mature structural and catalytic RNAs such as the snRNAs that splice pre-mRNA, rRNAs, and tRNAs that function in translation, swnoRNAs that guide modification of rRNAs, and individual mRNAs, whose expression they regulate	Drosophila melanogaster	mature RNA	staggered breaks with 2 nt, 3'-overhanging ends and 5'-phosphate and 3'-hydroxy termini	?
dsRNA + H2O	cleavage of fully or partially double-stranded RNA precursors into mature structural and catalytic RNAs such as the snRNAs that splice pre-mRNA, rRNAs, and tRNAs that function in translation, swnoRNAs that guide modification of rRNAs, and individual mRNAs, whose expression they regulate	Caenorhabditis elegans	mature RNA	staggered breaks with 2 nt, 3'-overhanging ends and 5'-phosphate and 3'-hydroxy termini	?
dsRNA + H2O	cleavage of fully or partially double-stranded RNA precursors into mature structural and catalytic RNAs such as the snRNAs that splice pre-mRNA, rRNAs, and tRNAs that function in translation, swnoRNAs that guide modification of rRNAs, and individual mRNAs, whose expression they regulate	Aquifex aeolicus	mature RNA	staggered breaks with 2 nt, 3'-overhanging ends and 5'-phosphate and 3'-hydroxy termini	?
additional information	specificity for A-form dsRNA, enzyme is loosely associated with ribosomes	Escherichia coli	?	-	?

Subunits

Subunits	Comment	Organism
More	model of class I endonuclease domain dimer	Escherichia coli
More	model of class I endonuclease domain dimer	Aquifex aeolicus
More	model of class III endonuclease domain dimer	Drosophila melanogaster
More	model of class III endonuclease domain dimer	Homo sapiens
More	model of class III endonuclease domain dimer	Arabidopsis thaliana
More	model of class III endonuclease domain dimer	Caenorhabditis elegans

Synonyms

Synonyms	Comment	Organism
Dicer	-	Drosophila melanogaster
Dicer	-	Escherichia coli
Dicer	-	Homo sapiens
Dicer	-	Arabidopsis thaliana
Dicer	-	Aquifex aeolicus
ribonuclease D	-	Aquifex aeolicus
RNase D	-	Aquifex aeolicus
RNase III	-	Drosophila melanogaster
RNase III	-	Escherichia coli
RNase III	-	Homo sapiens
RNase III	-	Arabidopsis thaliana
RNase III	-	Aquifex aeolicus
RNase O	-	Aquifex aeolicus

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Release 2023.1 (January 2023)