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Literature summary for 3.1.26.3 extracted from

  • Watson, N.; Apirion, D.
    Molecular cloning of the gene for the RNA-processing enzyme RNase III of Escherichia coli (1985), Proc. Natl. Acad. Sci. USA, 82, 849-853.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the rnc gene in Escherichia coli Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded RNA + H2O Escherichia coli cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA 5'-phosphooligonucleotides
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of the overexpressed protein Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded RNA + H2O
-
Escherichia coli 5'-phosphooligonucleotides
-
?
double-stranded RNA + H2O cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA Escherichia coli 5'-phosphooligonucleotides
-
?

Subunits

Subunits Comment Organism
dimer 2 * 25000-25300, SDS-PAGE Escherichia coli