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Literature summary for 3.1.26.3 extracted from

  • Takeshita, D.; Zenno, S.; Lee, W.C.; Nagata, K.; Saigo, K.; Tanokura, M.
    Crystallization and preliminary X-ray analysis of the C-terminal RNase III domain of human Dicer (2006), Acta Crystallogr. Sect. F, 62, 402-404.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
region encoding the RNase IIIb domain (residues 1660-1852) expressed as His-tag fusion protein in Escherichia coli BL21 star (DE3) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant protein including His-tag by sitting drop vapor diffusion method Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded RNA + H2O Homo sapiens involved in the production of short interfering RNAs (siRNAs) and for the processing of precursor miRNAs (pre-miRNAs) into microRNAs (miRNAs) 5'-phosphooligonucleotides
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UPY3
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-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded RNA + H2O involved in the production of short interfering RNAs (siRNAs) and for the processing of precursor miRNAs (pre-miRNAs) into microRNAs (miRNAs) Homo sapiens 5'-phosphooligonucleotides
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 ?

Synonyms

Synonyms Comment Organism
Dicer Dicer protein contains two RNase III domains (RNase IIIa and RNase IIIb) Homo sapiens