EC Number   |
Protein Variants |
Reference |
|---|
   3.1.26.3 | D114A |
mutant exhibits catalytic activity in vitro in 10 mM Mg2+ buffer that is comparable to that of the wild-type enzyme. At 1 mM Mg2+, the activity is significantly lower, KM-value for Mg2+ is about 2.8fold larger than the wild-type value |
664016 |
| 3.1.26.3 | D1217N/D1614N |
site-directed mutagenesis of Dicer-2 |
716223 |
| 3.1.26.3 | D128A |
site-directed mutagenesis, the D128A mutation in both RNase III subunits, D128A/D128'A, causes an 83fold increase in KD in the interaction of RNase III with YmdB |
752139 |
| 3.1.26.3 | D1709A |
strongly reduced dsRNA cleavage activity |
681461 |
| 3.1.26.3 | D1713A |
no significant effect on the cleavage activity |
681461 |
| 3.1.26.3 | D1713K |
no significant effect on the cleavage activity |
681461 |
| 3.1.26.3 | D1810A |
reduced dsRNA cleavage activity |
681461 |
| 3.1.26.3 | D218A |
site-directed mutagenesis, inactive mutant |
716905 |
| 3.1.26.3 | D44N |
mutant enzyme with greatly reduced activity |
664543 |
| 3.1.26.3 | D44N |
mutant with greatly reduced activity |
664543 |
