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Literature summary for 3.1.26.3 extracted from
- Functional characterization of the RNase III gene of rock bream iridovirus (2008), Arch. Virol., 153, 1651-1656.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) | Rock bream iridovirus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | completely inhibited at 200 mM NaCl (within physiological ranges) irrespective of Mg2+ concentrations (0.5-20 mM) | Rock bream iridovirus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dsRNA substrate is cleaved by the RBIV RNase III at high concentrations of Mg2+ (5-20 mM) and at low salt concentration (50 mM) | Rock bream iridovirus | |
| Mn2+ | substrate dsRNA is cleaved at low concentrations of Mn2+ (0.5-1 mM) at low salt concentration (50 mM) and is cleaved by increasing Mn2+ (5-20 mM) at 200 mM salt | Rock bream iridovirus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30900 | - |
calculated from sequence | Rock bream iridovirus |
| 31090 | - |
of the His-tag fused protein, SDS-PAGE | Rock bream iridovirus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rock bream iridovirus | Q5YF04 | RBIV | - |
| Rock bream iridovirus RBIV | Q5YF04 | RBIV | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| six-His fusion protein is purified from the soluble fraction using Ni-nitrilotriacetic resin | Rock bream iridovirus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded RNA + H2O | - |
Rock bream iridovirus | 5'-phosphooligonucleotides | - |
? | |
| double-stranded RNA + H2O | - |
Rock bream iridovirus RBIV | 5'-phosphooligonucleotides | - |
? | |
| dsDNA + H2O | - |
Rock bream iridovirus | ? | - |
? | |
| dsDNA + H2O | - |
Rock bream iridovirus RBIV | ? | - |
? | |
| single-stranded RNA + H2O | - |
Rock bream iridovirus | 5'-phosphooligonucleotides | - |
? | |
| single-stranded RNA + H2O | - |
Rock bream iridovirus RBIV | 5'-phosphooligonucleotides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RBIV RNase III | - |
Rock bream iridovirus |
| RNase III | - |
Rock bream iridovirus |
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