Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | substrate binding is inhibited by a specific sequence within the dsRNA stem, overview | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, substrate binding with diverse RNA substrates | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | enzyme is involved in RNA processing and RNA interference, i.e. RNAi, regulation by a combination of primary and tertiary structural elements allowing a substrate-specific binding and cleavage efficiency | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dsRNA + H2O | diverse model RNA substreates, enzyme cleaves specifically RNA stems capped with the conserved AGNN tetraloop, the dsRNA sequence adjacent to the tetraloop regulates enzyme activity by interfering with substrate binding, sequences surrounding the cleavage site directly influence the cleavage efficiency, a minimum substrate length is required | Saccharomyces cerevisiae | mature RNA | - |
? | |
additional information | enzyme is involved in RNA processing and RNA interference, i.e. RNAi, regulation by a combination of primary and tertiary structural elements allowing a substrate-specific binding and cleavage efficiency | Saccharomyces cerevisiae | ? | - |
? | |
additional information | substrate specificity, overview, RNA substrate with introduced sequences stabilizing the RNA helix enhances binding while the turnover rate is reduced, thus substrate binding becomes rate-limiting | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the double-stranded RNA specific RNase III family of endoribonucleases | Saccharomyces cerevisiae |
RNase III | - |
Saccharomyces cerevisiae |
Rnt1p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |