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Literature summary for 3.1.26.3 extracted from

  • Fang, P.; Wang, J.; Li, X.; Guo, M.; Xing, L.; Cao, X.; Zhu, Y.; Gao, Y.; Niu, L.; Teng, M.
    Crystallization and preliminary X-ray analysis of Escherichia coli RNase G (2009), Acta Crystallogr. Sect. F, 65, 586-588.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Escherichia coli K-12

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour-diffusion method, 1.2 M magnesium sulfate and 0.1 M MES pH 6.5, purified recombinant Escherichia coli RNase G is crystallized in the cubic space group F432, with unit-cell parameters a = b = c = 219.84 A, resolution 3.4 A Escherichia coli K-12

Protein Variants

Protein Variants Comment Organism
R206H manipulations during cloning Escherichia coli K-12

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
SDS-PAGE Escherichia coli K-12

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12 P0A9J0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni sepharose high performance chromatography, Q high performance chromatography Escherichia coli K-12

Synonyms

Synonyms Comment Organism
RNase G
-
Escherichia coli K-12