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Literature summary for 3.1.26.3 extracted from
- Characterization of a chlorella virus PBCV-1 encoded ribonuclease III (2003), Virology, 317, 73-83.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| orf A464R, expression in Escherichia coli ER2566 using the chitin-binding intein system | Paramecium bursaria Chlorella virus-1 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Paramecium bursaria Chlorella virus-1 |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on, optimum concentration is about 5 mM | Paramecium bursaria Chlorella virus-1 | |
| Mn2+ | can substitute for Mg2+, best at 1 mM, leads to production of additional cleavage products | Paramecium bursaria Chlorella virus-1 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
2 * 31000, recombinant enzyme, intein tag is cleaved off | Paramecium bursaria Chlorella virus-1 |
| 64000 | - |
recombinant, non-tagged enzyme, native PAGE | Paramecium bursaria Chlorella virus-1 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Paramecium bursaria Chlorella virus-1 | phylogenetic analysis, enzyme might be important for virus replication | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paramecium bursaria Chlorella virus-1 | - |
i.e. PBCV-1, gene a464r | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant intein-tagged enzyme from Escherichia coli, to homogeneity, intein-tag is cleaved off | Paramecium bursaria Chlorella virus-1 |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | enzyme is expressed during the very early infection stage of the virus, 5 min p.i., and disappears 20 min p.i. | Paramecium bursaria Chlorella virus-1 | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified recombinant non-tagged enzyme, over one year without loss of activity | Paramecium bursaria Chlorella virus-1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dsRNA + H2O | model substrate | Paramecium bursaria Chlorella virus-1 | mature RNA | product determination | ? | |
| additional information | phylogenetic analysis, enzyme might be important for virus replication | Paramecium bursaria Chlorella virus-1 | ? | - |
? | |
| additional information | substrate cleavage specificity | Paramecium bursaria Chlorella virus-1 | ? | - |
? | |
| R1.1 RNA + H2O | based on the R1.1 processing signal, which is encoded in the phage T7 genetic early region between genes 1.0 and 1.1, several cleavage sites | Paramecium bursaria Chlorella virus-1 | fragments of R1.1 RNA | product determination | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 31000, recombinant enzyme, intein tag is cleaved off | Paramecium bursaria Chlorella virus-1 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase III | - |
Paramecium bursaria Chlorella virus-1 |
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