Cloned (Comment) | Organism |
---|---|
gene rnc, DNA and amino acid sequence determination and analysis, cloning in the overexpression vector pIJ8600 | Streptomyces antibioticus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a disruption mutant of the chromosomal RNase III gene rnc by insertional mutagenesis, the mutant strain shows reduced actinomycin production. Complementation of mutant strain JSE1980 with pJSE1995 encoding the wild-type rnc gene restores actinomycin production to nearly wild-type levels | Streptomyces antibioticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces antibioticus | U3GP13 | gene rnc | - |
Streptomyces antibioticus IMRU 3720 | U3GP13 | gene rnc | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Streptomyces antibioticus | - |
Synonyms | Comment | Organism |
---|---|---|
endoribonuclease III | - |
Streptomyces antibioticus |
RNase III | - |
Streptomyces antibioticus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme RNase III is a member of the ubiquitous family of double-strand-specific endoribonucleases. Streptomyces coelicolor and Streptomyces antibioticus are not closely related species within the genus | Streptomyces antibioticus |
malfunction | a gene disruption mutant strain does not produce full-length or truncated forms of RNase III and grows more vigorously than its parent on actinomycin production medium but produces significantly lower levels of actinomycin. Complementation of the rnc disruption with the wild-type rnc gene from Streptomyces antibioticus restores actinomycin production to nearly wild-type levels | Streptomyces antibioticus |
physiological function | the enzyme is required for and regulates antibiotic production | Streptomyces antibioticus |