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3.1.26.3: ribonuclease III

This is an abbreviated version!
For detailed information about ribonuclease III, go to the full flat file.

Word Map on EC 3.1.26.3

Reaction

Endonucleolytic cleavage to a 5'-phosphomonoester =

Synonyms

Aa-RNase III, AbsB, At1g55140, At3g13740, AtRTL1, AtRTL2, AtRTL3, BB_0705, BCG-RNase III, Bm-RNase III, cgR_1959, class 1 RNase III, DCL1, DCL2, DCL3, DCL4, DCR-1, Dicer, Dicer 1, Dicer-1, Dicer-2, Dicer1, double strand-specific endoRNase, Drosha, dsRNA-specific class 1 RNase III-like endoribonuclease, E. coli RNase III, Ec-RNase III, endoribonuclease III, endoribonucleases III, exodeoxyribonuclease III, HCS protein, Hs-Dicer, Hs-Drosha, KREPB10, KREPB9, Lac-RNase III, mini-III, mitochondrial RNA precursor-processing endonuclease 1, More, mRPN1, nuclease, ribo-, D, p241, Pac1p, RBIV RNase III, ribonuclease D, ribonuclease III, ribonuclease III-like protein, ribonuclease type III, RNase D, RNase G, RNase III, RNase III domain A, RNase III double-stranded RNA endonuclease, RNase III endonuclease, RNase III-like protein, RNase O, RNase3, rnc, RNC1, RNC3, RNC4, rncS, RNT1, Rnt1p, Rnt1p RNase III, Sa-RNase III, Sc-Rnt1p, SmRNase III, Tb09.160.3050, Tb927.8.5700, Tm-RNase III, TNase III

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.3 ribonuclease III

Molecular Weight

Molecular Weight on EC 3.1.26.3 - ribonuclease III

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
103000
-
recombinant GST-tagged wild-type homodimer, gel filtration
108000
-
x * 108000, calculated from the deduced amino acid sequence for AtRTL3
110000
17100
2 * 17100, His-tagged monomer, calculated
25600
27900
-
homodimer of 243 amino acid polypeptides
29000
30900
calculated from sequence
31000
Paramecium bursaria Chlorella virus-1
-
2 * 31000, recombinant enzyme, intein tag is cleaved off
31090
of the His-tag fused protein, SDS-PAGE
33000
-
x * 33000, calculated from the deduced amino acid sequence for AtRTL1
38100
gel filtration
40000 - 55000
41000
-
RNase O, gel filtration
45000
-
2 * 45000, subunit mass calculated from the deduced amino acid sequence for AtRTL2, native mass by gel filtration, forms highly salt resistant dimers, dimer formation through disulfide bonds, dimers can be disrupted upon DTT treatment
50000 - 60000
-
gel filtration
54000
-
recombinant His-tagged mutant homodimer, gel filtration
55000
-
2 * 55000, amino acid sequence calculation
57300
-
purified recombinant His-tagged wild-type enzyme, gel filtration
64000
Paramecium bursaria Chlorella virus-1
-
recombinant, non-tagged enzyme, native PAGE
78000
-
recombinant His-tagged and GST-tagged wild-type/mutant heterodimer, gel filtration