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3.1.26.3: ribonuclease III

This is an abbreviated version!
For detailed information about ribonuclease III, go to the full flat file.

Word Map on EC 3.1.26.3

Reaction

Endonucleolytic cleavage to a 5'-phosphomonoester =

Synonyms

Aa-RNase III, AbsB, At1g55140, At3g13740, AtRTL1, AtRTL2, AtRTL3, BB_0705, BCG-RNase III, Bm-RNase III, cgR_1959, class 1 RNase III, DCL1, DCL2, DCL3, DCL4, DCR-1, Dicer, Dicer 1, Dicer-1, Dicer-2, Dicer1, double strand-specific endoRNase, Drosha, dsRNA-specific class 1 RNase III-like endoribonuclease, E. coli RNase III, Ec-RNase III, endoribonuclease III, endoribonucleases III, exodeoxyribonuclease III, HCS protein, Hs-Dicer, Hs-Drosha, KREPB10, KREPB9, Lac-RNase III, mini-III, mitochondrial RNA precursor-processing endonuclease 1, More, mRPN1, nuclease, ribo-, D, p241, Pac1p, RBIV RNase III, ribonuclease D, ribonuclease III, ribonuclease III-like protein, ribonuclease type III, RNase D, RNase G, RNase III, RNase III domain A, RNase III double-stranded RNA endonuclease, RNase III endonuclease, RNase III-like protein, RNase O, RNase3, rnc, RNC1, RNC3, RNC4, rncS, RNT1, Rnt1p, Rnt1p RNase III, Sa-RNase III, Sc-Rnt1p, SmRNase III, Tb09.160.3050, Tb927.8.5700, Tm-RNase III, TNase III

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.3 ribonuclease III

Crystallization

Crystallization on EC 3.1.26.3 - ribonuclease III

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
12 three-dimensional structures of bacterial RNase III in various forms have been reported
Aquifex aeolicus RNase III also has been cocrystallized with dsRNA or specific hairpin substrates
crystal structure analysis of wild-type enzyme and D44N mutant enzyme
crystal structure of Aquifex aeolicus mutant enzyme D44N with double-stranded RNA bound in its catalytic valley
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hanging-drop vapour-diffusion method at 19°C. Crystallisation of the mutant enzyme E110Q in complex with dsRNA 2-2 formed by self-complementary sequence 5'-CGAACUUCGCG-3', complex of wild-type enzyme with dsRNA 3-3 formed by self-complementary sequence 5'-AAAUAUAUAUUU-3', complex of wild-type enzyme with dsRNA 4-4 formed by self-complementary sequence 5'-CGCGAAUUCGCG-3' and complex of mutant enzyme E110Q with dsRNA 4-4 formed by self-complementary sequence 5'-CGCGAAUUCGCG-3'
in complex with dsRNA, in the presence of Mg2+ or Mn2+ one metal ion bound per polypeptide chain
the structure of Aa-D44N RNase III in complex with a RNA product is determined at a resolution of 2.05 A
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three crystal structures of RNase III in complex with double-stranded RNA are solved at resolutions ranging from 1.7 to 2.9 A
12 three-dimensional structures of bacterial RNase III in various forms have been reported
hanging-drop vapour-diffusion method, 1.2 M magnesium sulfate and 0.1 M MES pH 6.5, purified recombinant Escherichia coli RNase G is crystallized in the cubic space group F432, with unit-cell parameters a = b = c = 219.84 A, resolution 3.4 A
crystal structure analysis, PDB ID 2ffl, crystallographic analysis of the minimal Dicer of Giardia intestinalis, containing the PAZ and tandem RNase III domainsl
recombinant protein including His-tag by sitting drop vapor diffusion method
recombinant RNase IIIb-dsRBD fragment by sitting drop vapor diffusion method
handing drop vapor diffusion method, crystallization of a catalytically active fragment of mouse Dicer, containing the RNase IIIb and dsRNA binding domains, in its apo and Cd2+-bound forms
crystal structure analysis, PDB ID 2A11
crystal structure of enzyme Rnt1p in complex with a G2 loop
crystal structure analysis, PDB ID 1O0W
RNA-free structure of full length RNase III, comparison with crystal structures of Aquifex aeolicus RNase-dsRNA complex indicates dramatic conformational changes upon dsRNA binding
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